Construct: sgRNA BRDN0001148426

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAGCAAAAGATTTGTACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK2 (5747)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75545

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 140744657 - PTK2 NNNGCAAAAGATTTGTACAC NGG 0 1.0 Tier I
2 NC_000010.11 10 89400473 - IFIT1 NNNGCAAAAGACTTGTACAC NGG 1 0.9474 Tier II
3 NC_000011.10 11 119247941 + CBL NNNGCAAAAGACTTGTACAC NGG 1 0.9474 Tier II
4 NC_000001.11 1 190296866 + BRINP3 NNNGTAAAAAATTTGTACAC NGG 2 0.5939 Tier II
5 NC_000010.11 10 13506544 + BEND7 NNNGCAGAAGAATTGTACAC NGG 2 0.5647 Tier II
6 NC_000022.11 22 28146179 + TTC28 NNNGCAAAATATTTGTATAC NGG 2 0.5223 Tier II
7 NC_000006.12 6 69778473 - LMBRD1 NNNGCAAAAGACTTGTAAAC NGG 2 0.5101 Tier II
8 NC_000001.11 1 84155334 + PRKACB NNNGTGAAAGATTTGTACAC NGG 2 0.4545 Tier II
9 NC_000021.9 21 15058441 - NRIP1 NNNGCAATAGATTTATACAC NGG 2 0.4034 Tier II
10 NC_000011.10 11 36491206 - TRAF6 NNNGCAATAGATTTGTATAC NGG 2 0.2755 Tier II
11 NC_000002.12 2 105858885 + NCK2 NNNGCAAAACATTTGTATAC NGG 2 0.2571 Tier II
12 NC_000002.12 2 151424566 - RIF1 NNNCCAACAGATTTGTACAC NGG 2 0.2269 Tier II
13 NC_000003.12 3 55535496 + ERC2 NNNGCAAAAGATTTATACCC NGG 2 0.1947 Tier II
14 NC_000010.11 10 103803612 + SH3PXD2A NNNGCAAAAGATTTGAACCC NGG 2 0.1881 Tier II
15 NC_000015.10 15 33396628 - RYR3 NNNGCATTAGATTTGTACAC NGG 2 0.1875 Tier II
16 NC_000004.12 4 181602073 - TENM3 NNNCCAAAAGATTTGTCCAC NGG 2 0.0934 Tier II
17 NC_000002.12 2 120151516 + EPB41L5 NNNGCAAAAGACTTGTACAC NGA 2 0.0658 Tier II
18 NC_000007.14 7 75997907 + STYXL1 NNNGCAAAAGACTTGTACAC NGA 2 0.0658 Tier II
19 NC_000010.11 10 37807627 - ZNF248 NNNGCAAAAGACTTGTACAC NGA 2 0.0658 Tier II
20 NC_000011.10 11 63176409 - SLC22A25 NNNGCAAAAGACTTGTACAC NGA 2 0.0658 Tier II
21 NC_000013.11 13 92840804 - GPC5 NNNGCAAAAGACTTGTACAC NGA 2 0.0658 Tier II
22 NC_000016.10 16 20633490 + ACSM1 NNNGCAAAAGACTTGTACAC NGA 2 0.0658 Tier II
23 NC_000023.11 X 114588682 - HTR2C NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier II
24 NC_000005.10 5 19642144 + CDH18 NNNGCAAAATATTTGTACAC NGA 2 0.0564 Tier II
25 NC_000018.10 18 6231609 - L3MBTL4 NNNGTAAAAGATTTGTACAC NGA 2 0.0442 Tier II
26 NC_000018.10 18 26149271 + PSMA8 NNNGCAAAAGATTTGTACTC NGA 2 0.0374 Tier II
27 NC_000023.11 X 29586736 - IL1RAPL1 NNNGCAAAAGAGTTGTACAC NGA 2 0.0347 Tier II
28 NC_000012.12 12 85101462 - LRRIQ1 NNNGCAAAAGATTTGTACAA NGA 2 0.0347 Tier II
29 NC_000020.11 20 2512132 + ZNF343 NNNGCACAAGATTTGTACAC NGA 2 0.0304 Tier II
30 NC_000007.14 7 114460764 + FOXP2 NNNGCAAATGATTTGTACAC NTG 2 0.0234 Tier II
31 NC_000012.12 12 772386 + WNK1 NNNGCAAAATATTTGTACAC NGT 2 0.0131 Tier II
32 NC_000008.11 8 70914537 - XKR9 NNNGCAAAAGATTTGTACAA NGC 2 0.0111 Tier II
33 NC_000014.9 14 64838583 + SPTB NNNGCAAAAGATTTGTACAG NCG 2 0.0063 Tier II
34 NC_000003.12 3 97327023 - EPHA6 NNNGCAAAAGATTTGTTCAC NGT 2 0.0022 Tier II
35 NC_000009.12 9 25504009 - LOC107987056 NNNGCAAAAAATTTGAACAC NGG 2 0.8485 Tier III
36 NC_000010.11 10 35795906 + PCAT5 NNNGAAAAAGATTTGAACAC NGG 2 0.5195 Tier III
37 NC_000012.12 12 84087404 - LOC107984536 NNNCCAAAAGATTTGAACAC NGG 2 0.4813 Tier III
38 NC_000016.10 16 65462236 + LINC00922 NNNGCAATACATTTGTACAC NGG 2 0.1714 Tier III
39 NC_000002.12 2 90190559 + IGKV1D-42 NNNGCAAAAGATTTGCACCC NGG 2 0.1379 Tier III
40 NC_000002.12 2 90190559 + IGK NNNGCAAAAGATTTGCACCC NGG 2 0.1379 Tier III
41 NC_000008.11 8 93445021 - CIBAR1-DT NNNGCAAATGATTTGTGCAC NGG 2 0.1059 Tier III
42 NC_000001.11 1 199020222 + LINC01221 NNNGCAAAAGTTTTGTACAC NAG 2 0.0798 Tier III
43 NC_000001.11 1 192384400 + LOC124904472 NNNGCAAAAGACTTGTACAC NGA 2 0.0658 Tier III
44 NC_000018.10 18 35453844 + ZNF24TR NNNGCAAAAGACTTGTACAC NGA 2 0.0658 Tier III
45 NC_000004.12 4 49187100 - LOC101927209 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
46 NC_000021.9 21 8784684 - LINC01666 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
47 NC_000021.9 21 9110287 + TEKT4P2 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
48 NC_000021.9 21 9350945 - LOC101930100 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
49 NC_000022.11 22 11948233 + LOC102723769 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
50 NT_113796.3 14 105473 + LOC105379271 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
51 NT_113889.1 77968 + LOC100233156 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
52 NT_167215.1 3 54141 + LOC101928669 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
53 NT_187361.1 1 25086 - LOC105379854 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
54 NT_187513.1 84073 + LOC105379566 NNNGCAAAAGATTTGAACAC NGA 2 0.0631 Tier III
55 NC_000005.10 5 15227707 - LINC02149 NNNACAAAAGATTTGTACAC NGA 2 0.0625 Tier III
56 NC_000020.11 20 40573870 + LOC102724968 NNNGCAAAAGATTTGTATAC NGA 2 0.0446 Tier III
57 NC_000008.11 8 88663345 - LOC105375630 NNNCCAAAAGATTTGTACAC NGA 2 0.0368 Tier III
58 NC_000008.11 8 88663345 - LOC124901973 NNNCCAAAAGATTTGTACAC NGA 2 0.0368 Tier III
59 NC_000003.12 3 97327023 - LOC101929278 NNNGCAAAAGATTTGTTCAC NGT 2 0.0022 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 73264219 - Ptk2 NNNGCAAAAGATTTGTTCAC NGG 1 0.1333 Tier I
2 NC_000072.6 6 38209123 - D630045J12Rik NNNGCAAAACATATGTACAC NGG 2 0.2769 Tier II
3 NC_000071.6 5 138987368 + Pdgfa NNNGCAAGAGATTTGTACAC NAG 2 0.1901 Tier II
4 NC_000068.7 2 90546680 - Ptprj NNNGCAGAAGATGTGTACAC NGG 2 0.1841 Tier II
5 NC_000070.6 4 138313401 - Pink1 NNNGCCAAAGATGTGTACAC NGG 2 0.1186 Tier II
6 NC_000072.6 6 141458295 - Pde3a NNNGCAAAACATTCGTACAC NGG 2 0.1143 Tier II
7 NC_000068.7 2 161755855 + Ptprt NNNGCAAAAGATTTGTCCCC NGG 2 0.0365 Tier II
8 NC_000068.7 2 75592493 + Gm50595 NNNTCAAAAGATTTGTACAG NGG 2 0.0214 Tier II
9 NC_000076.6 10 103184773 + Lrriq1 NNNGCAAAAGATTTGTACAT NGA 2 0.0208 Tier II
10 NC_000074.6 8 90886270 + Chd9 NNNGCAAAAGATATGTACAC NGC 2 0.0154 Tier II
11 NC_000067.6 1 177294837 - Gm36904 NNNGCAAAAGATTTGTAAAC NAG 2 0.1396 Tier III
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Other clones with same target sequence:

(none)