Construct: sgRNA BRDN0001148427
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGGTGGGTTTACCAGCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKFB1 (5207)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77631
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
54963325 |
- |
PFKFB1 |
NNNGTGGGTTTACCAGCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
127276295 |
- |
ERCC3 |
NNNGTGGGTTTGCCAGCTTG |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000023.11 |
X |
57208923 |
- |
FAAH2 |
NNNGTGGGTTTACCAGCATG |
NGG |
2 |
0.3077 |
Tier II |
| 4 |
NC_000009.12 |
9 |
138009503 |
+ |
CACNA1B |
NNNGTGGGTTTGCAAGCTCG |
NGG |
2 |
0.2528 |
Tier II |
| 5 |
NC_000021.9 |
21 |
34902816 |
+ |
RUNX1 |
NNNGTGGGTTTTCCAGCTCC |
NGG |
2 |
0.1429 |
Tier II |
| 6 |
NC_000019.10 |
19 |
19269862 |
+ |
TM6SF2 |
NNNGTGGGTTTCCCAGCTCC |
NGG |
2 |
0.1128 |
Tier II |
| 7 |
NC_000003.12 |
3 |
147405173 |
+ |
ZIC4 |
NNNTTGGGTTTCCCAGCTCG |
NGG |
2 |
0.0957 |
Tier II |
| 8 |
NC_000023.11 |
X |
40645588 |
+ |
CXorf38 |
NNNGTGGGTTTACCGGCTCG |
NGA |
2 |
0.0451 |
Tier II |
| 9 |
NC_000002.12 |
2 |
101732378 |
+ |
MAP4K4 |
NNNGTGGTTTTACCATCTCG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000007.14 |
7 |
30647978 |
+ |
LOC124901609 |
NNNGTGGGTTAACCAGCTCT |
NGG |
2 |
0.525 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
150608644 |
+ |
Pfkfb1 |
NNNGTGGGTTTACCAGCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000083.6 |
17 |
86181423 |
+ |
Prkce |
NNNGAGGGTTTACCAGCACG |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000078.6 |
12 |
3387803 |
+ |
Kif3c |
NNNCTGGGTGTACCAGCTCG |
NGG |
2 |
0.2647 |
Tier II |
Other clones with same target sequence:
(none)
