Construct: sgRNA BRDN0001148428

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCTGATGATTATCACCCGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IDNK (414328)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75867

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 83628910 + IDNK NNNTGATGATTATCACCCGG NGG 0 1.0 Tier I
2 NC_000016.10 16 1554948 - TMEM204 NNNTGATGACAATCACCCGG NGG 2 0.4 Tier I
3 NC_000016.10 16 1554948 - IFT140 NNNTGATGACAATCACCCGG NGG 2 0.4 Tier II
4 NC_000022.11 22 43384751 + LOC105373053 NNNTGATTATTATCACCTGG NGG 2 0.4018 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 10321600 + Myo16 NNNTGATGCTTATCACCCAG NGG 2 0.4082 Tier II
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Other clones with same target sequence:

(none)