Construct: sgRNA BRDN0001148430
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGAGAATCCAGGTTCTGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PKMYT1 (9088)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77279
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
2976938 |
+ |
PKMYT1 |
NNNAGAATCCAGGTTCTGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
133545880 |
- |
ADAMTSL2 |
NNNAGAGTCCAAGTTCTGCG |
NGG |
2 |
0.6588 |
Tier II |
| 3 |
NC_000003.12 |
3 |
86063446 |
- |
CADM2 |
NNNAGAATCCAGGTTCTTCA |
NGG |
2 |
0.625 |
Tier II |
| 4 |
NC_000003.12 |
3 |
14168460 |
+ |
XPC |
NNNAGAATCAAGGTTCTGCT |
NGG |
2 |
0.6067 |
Tier II |
| 5 |
NC_000010.11 |
10 |
35414298 |
- |
CCNY |
NNNAGCACCCAGGTTCTGCG |
NGG |
2 |
0.4545 |
Tier II |
| 6 |
NC_000003.12 |
3 |
55546516 |
+ |
ERC2 |
NNNAGAATCCAGGTTCTGTA |
NGG |
2 |
0.4327 |
Tier II |
| 7 |
NC_000001.11 |
1 |
231956930 |
- |
DISC1 |
NNNAGAGTCCAGTTTCTGCG |
NGG |
2 |
0.2118 |
Tier II |
| 8 |
NC_000001.11 |
1 |
43350727 |
+ |
MPL |
NNNAGAATCCAGGTTCTGGC |
NGG |
2 |
0.0536 |
Tier II |
| 9 |
NC_000004.12 |
4 |
21652293 |
- |
KCNIP4 |
NNNAGAATCCAGGTTCTGCT |
NGA |
2 |
0.0486 |
Tier II |
| 10 |
NC_000001.11 |
1 |
10064562 |
+ |
UBE4B |
NNNAGAATCCAGGTGCTGCC |
NGG |
2 |
0.0214 |
Tier II |
| 11 |
NC_000001.11 |
1 |
179782539 |
- |
FAM163A |
NNNAGAATCCAGATTCTGCG |
NGC |
2 |
0.0205 |
Tier II |
| 12 |
NC_000023.11 |
X |
86296828 |
+ |
DACH2 |
NNNAGAATCCAGGTTGTGGG |
NGG |
2 |
0.0192 |
Tier II |
| 13 |
NC_000001.11 |
1 |
231956930 |
- |
TSNAX-DISC1 |
NNNAGAGTCCAGTTTCTGCG |
NGG |
2 |
0.2118 |
Tier III |
| 14 |
NC_000008.11 |
8 |
37359156 |
+ |
LOC124901930 |
NNNAGAATCCAGGTTTAGCG |
NGG |
2 |
0.1641 |
Tier III |
| 15 |
NC_000002.12 |
2 |
2757898 |
- |
LOC105373390 |
NNNAGAATCCAGGTTCTCCG |
NTG |
2 |
0.0186 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
43062980 |
+ |
Atp10b |
NNNAGAATACAGGTTCTGCA |
NGG |
2 |
0.8036 |
Tier II |
| 2 |
NC_000082.6 |
16 |
67380532 |
- |
Cadm2 |
NNNAGAATCTAGGTTCTGCT |
NGG |
2 |
0.6588 |
Tier II |
| 3 |
NC_000080.6 |
14 |
66348693 |
+ |
Stmn4 |
NNNAGGATACAGGTTCTGCG |
NGG |
2 |
0.6122 |
Tier II |
| 4 |
NC_000075.6 |
9 |
92257607 |
- |
Plscr1 |
NNNAGAATCCAGGTTCTGTG |
NGG |
1 |
0.4615 |
Tier II |
| 5 |
NC_000074.6 |
8 |
111853150 |
- |
Cfdp1 |
NNNAGAATCCAGCTTATGCG |
NGG |
2 |
0.4211 |
Tier II |
| 6 |
NC_000067.6 |
1 |
20448335 |
+ |
Pkhd1 |
NNNAGAATTCAGGTTCTGAG |
NGG |
2 |
0.375 |
Tier II |
| 7 |
NC_000084.6 |
18 |
80307310 |
+ |
Kcng2 |
NNNAGAAACCATGTTCTGCG |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000077.6 |
11 |
67630419 |
- |
Gas7 |
NNNTGAATCCAGGTTCTGTG |
NGG |
2 |
0.2937 |
Tier II |
| 9 |
NC_000075.6 |
9 |
50809845 |
+ |
Alg9 |
NNNAGAATCCAGGCTCTGCA |
NGG |
2 |
0.2679 |
Tier II |
| 10 |
NC_000080.6 |
14 |
60702172 |
+ |
Spata13 |
NNNAGAATCCAGGCTCTGCA |
NGG |
2 |
0.2679 |
Tier II |
| 11 |
NC_000076.6 |
10 |
23143036 |
+ |
Eya4 |
NNNAGAATCCAGGTACTGAG |
NGG |
2 |
0.2481 |
Tier II |
| 12 |
NC_000071.6 |
5 |
37524157 |
- |
Stk32b |
NNNAGAATCCAGGTTCTTGG |
NGG |
2 |
0.0833 |
Tier II |
| 13 |
NC_000073.6 |
7 |
35400565 |
+ |
Cep89 |
NNNAGAATCCAGGTTCTGAG |
NTG |
2 |
0.0167 |
Tier II |
| 14 |
NC_000077.6 |
11 |
44459372 |
+ |
Ublcp1 |
NNNAGAATCCAGGGTCTGCA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000083.6 |
17 |
69483311 |
- |
Akain1 |
NNNAGAATCCAGGGTCTGGG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000072.6 |
6 |
65666548 |
+ |
Gm36144 |
NNNAGACTCCAGGTTCTGCA |
NGG |
2 |
0.4102 |
Tier III |
| 17 |
NC_000068.7 |
2 |
95465159 |
- |
Gm13794 |
NNNAGAATCCAGGTTCAGAG |
NGG |
2 |
0.2286 |
Tier III |
Other clones with same target sequence:
(none)
