Construct: sgRNA BRDN0001148432
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAACTATAAACGAGTGACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK3 (79646)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76293
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
168566024 |
- |
PANK3 |
NNNACTATAAACGAGTGACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
222568380 |
- |
FARSB |
NNNACTATAAAAGAGTGACT |
NGG |
1 |
0.7143 |
Tier II |
| 3 |
NC_000001.11 |
1 |
224375606 |
- |
CNIH4 |
NNNACTAAAAAAGAGTGACT |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000003.12 |
3 |
188877191 |
- |
LPP |
NNNACTAAAAAAGAGTGACT |
NGG |
2 |
0.5714 |
Tier II |
| 5 |
NC_000007.14 |
7 |
40692091 |
- |
SUGCT |
NNNATTATTAACGAGTGACT |
NGG |
2 |
0.3818 |
Tier II |
| 6 |
NC_000023.11 |
X |
115593189 |
+ |
PLS3 |
NNNACTATGGACGAGTGACT |
NGG |
2 |
0.3704 |
Tier II |
| 7 |
NC_000003.12 |
3 |
100160000 |
+ |
CMSS1 |
NNNACAATAAACCAGTGACT |
NGG |
2 |
0.3649 |
Tier II |
| 8 |
NC_000001.11 |
1 |
3417617 |
+ |
PRDM16 |
NNNACTACAAACGAGGGACT |
NGG |
2 |
0.3462 |
Tier II |
| 9 |
NC_000014.9 |
14 |
94237493 |
- |
PPP4R4 |
NNNACTAAAAACCAGTGACT |
NGG |
2 |
0.3368 |
Tier II |
| 10 |
NC_000004.12 |
4 |
40981112 |
- |
APBB2 |
NNNACTATAAACAAGTTACT |
NGG |
2 |
0.2308 |
Tier II |
| 11 |
NC_000005.10 |
5 |
33821458 |
- |
ADAMTS12 |
NNNACTATAAATCAGTGACT |
NGG |
2 |
0.2267 |
Tier II |
| 12 |
NC_000006.12 |
6 |
55492661 |
- |
HMGCLL1 |
NNNACTATAAATGAGTGACT |
NAG |
2 |
0.1396 |
Tier II |
| 13 |
NC_000006.12 |
6 |
112222971 |
- |
LAMA4 |
NNNACTATAAACAAGTGACT |
NGA |
2 |
0.0641 |
Tier II |
| 14 |
NC_000018.10 |
18 |
10786155 |
- |
PIEZO2 |
NNNACTATAAATGAGTGACT |
NGC |
2 |
0.012 |
Tier II |
| 15 |
NC_000011.10 |
11 |
50268783 |
+ |
LOC646801 |
NNNTCTATAAACGAATGACT |
NGG |
2 |
0.5989 |
Tier III |
| 16 |
NC_000003.12 |
3 |
100160000 |
+ |
LOC105374009 |
NNNACAATAAACCAGTGACT |
NGG |
2 |
0.3649 |
Tier III |
| 17 |
NC_000005.10 |
5 |
152003668 |
- |
LINC01933 |
NNNACTATAAATGTGTGACT |
NGG |
2 |
0.2872 |
Tier III |
| 18 |
NC_000020.11 |
20 |
58645662 |
+ |
LOC107985410 |
NNNACTAGAAACGAGTGAGT |
NGG |
2 |
0.0917 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
35777810 |
+ |
Pank3 |
NNNACTATAAAAGAGTGACT |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000078.6 |
12 |
79073202 |
+ |
Plekhh1 |
NNNACTATAAAAGAGTGACT |
NGG |
1 |
0.7143 |
Tier II |
| 3 |
NC_000067.6 |
1 |
43103292 |
- |
AI597479 |
NNNACAATAAAAGAGTGACT |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000078.6 |
12 |
116290084 |
- |
Esyt2 |
NNNACTATAAAGAAGTGACT |
NGG |
2 |
0.4103 |
Tier II |
| 5 |
NC_000072.6 |
6 |
116272016 |
+ |
Zfand4 |
NNNACCATAAACCAGTGACT |
NGG |
2 |
0.3828 |
Tier II |
| 6 |
NC_000076.6 |
10 |
93688583 |
+ |
Ntn4 |
NNNTCTATAACCGAGTGACT |
NGG |
2 |
0.2545 |
Tier II |
| 7 |
NC_000086.7 |
X |
139944742 |
+ |
Rbm41 |
NNNACTATAAACGAATCACT |
NGG |
2 |
0.2215 |
Tier II |
| 8 |
NC_000079.6 |
13 |
47239174 |
- |
Rnf144b |
NNNACTATACACCAGTGACT |
NGG |
2 |
0.1404 |
Tier II |
Other clones with same target sequence:
(none)
