Construct: sgRNA BRDN0001148439
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGGACACAGACGCCGACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WNK2 (65268)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75670
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
93267853 |
+ |
WNK2 |
NNNGACACAGACGCCGACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
12705416 |
+ |
TNPO2 |
NNNGACACAGATGCCGACAG |
NGG |
2 |
0.2308 |
Tier II |
| 3 |
NC_000016.10 |
16 |
4846670 |
+ |
GLYR1 |
NNNGACACAGAAGCAGACCG |
NGG |
2 |
0.1587 |
Tier II |
| 4 |
NC_000003.12 |
3 |
129001379 |
+ |
CFAP92 |
NNNGACCCAGACGCCGACCG |
NGC |
2 |
0.0097 |
Tier II |
| 5 |
NC_000003.12 |
3 |
129001379 |
+ |
EFCC1 |
NNNGACCCAGACGCCGACCG |
NGC |
2 |
0.0097 |
Tier II |
| 6 |
NC_000009.12 |
9 |
93267853 |
+ |
LOC124902214 |
NNNGACACAGACGCCGACCG |
NGG |
0 |
1.0 |
Tier III |
| 7 |
NC_000006.12 |
6 |
156270252 |
- |
LOC101928923 |
NNNGACACAGATGCCGACAG |
NGG |
2 |
0.2308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
81166125 |
- |
Smoc1 |
NNNGAAAGAGACGCCGACCG |
NGG |
2 |
0.5969 |
Tier II |
| 2 |
NC_000076.6 |
10 |
5812964 |
- |
Mtrf1l |
NNNGACACAGAAGCCGACAG |
NGG |
2 |
0.3061 |
Tier II |
| 3 |
NC_000068.7 |
2 |
167474517 |
- |
Slc9a8 |
NNNGACACAGACGCAGACCA |
NGG |
2 |
0.2083 |
Tier II |
| 4 |
NC_000085.6 |
19 |
19830480 |
- |
Gm32469 |
NNNGACACAGACACAGACCG |
NGG |
2 |
0.2051 |
Tier III |
Other clones with same target sequence:
(none)
