Construct: sgRNA BRDN0001148440
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGGTGCACGTGATCACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IPPK (64768)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77154
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
92638121 |
- |
IPPK |
NNNGGTGCACGTGATCACAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
101013058 |
- |
MUC12 |
NNNGGTGCACTTGTTCACAC |
NGG |
2 |
0.2051 |
Tier I |
| 3 |
NC_000001.11 |
1 |
54251952 |
- |
SSBP3 |
NNNGCTCCACGTGATCACAC |
NGG |
2 |
0.5402 |
Tier II |
| 4 |
NC_000016.10 |
16 |
10909891 |
+ |
CIITA |
NNNGGTGCACGTCATCACAC |
NGG |
1 |
0.4211 |
Tier II |
| 5 |
NC_000006.12 |
6 |
136241942 |
- |
MTFR2 |
NNNGGGGCACGTCATCACAC |
NGG |
2 |
0.2406 |
Tier II |
| 6 |
NC_000002.12 |
2 |
1865684 |
+ |
MYT1L |
NNNGGTGCAGGTGATCACAT |
NGG |
2 |
0.1167 |
Tier II |
| 7 |
NC_000007.14 |
7 |
27963718 |
+ |
JAZF1 |
NNNGGTGCACGTCACCACAC |
NGG |
2 |
0.1148 |
Tier II |
| 8 |
NC_000017.11 |
17 |
43503527 |
- |
DHX8 |
NNNGGTGCACGTGATTACAC |
NCG |
2 |
0.033 |
Tier II |
| 9 |
NC_000005.10 |
5 |
31525366 |
- |
DROSHA |
NNNGGTGCACGTGACCACAC |
NCG |
2 |
0.0292 |
Tier II |
| 10 |
NC_000003.12 |
3 |
174691274 |
- |
NAALADL2 |
NNNGGTGCACGTCATCACAC |
NTG |
2 |
0.0164 |
Tier II |
| 11 |
NC_000008.11 |
8 |
135631113 |
- |
KHDRBS3 |
NNNGGTGCACGTCATCACAC |
NTG |
2 |
0.0164 |
Tier II |
| 12 |
NC_000012.12 |
12 |
128951265 |
- |
GLT1D1 |
NNNGGTGCACGTCATCACAC |
NTG |
2 |
0.0164 |
Tier II |
| 13 |
NC_000012.12 |
12 |
48560965 |
- |
OR5BS1P |
NNNGGTGCACGTGACCACAC |
NTG |
2 |
0.0106 |
Tier II |
| 14 |
NC_000016.10 |
16 |
84861789 |
- |
CRISPLD2 |
NNNGGTGCACGTGACCACAC |
NTG |
2 |
0.0106 |
Tier II |
| 15 |
NC_000003.12 |
3 |
57971815 |
+ |
LOC105377104 |
NNNGGTGCTCGAGATCACAC |
NGG |
2 |
0.48 |
Tier III |
| 16 |
NC_000014.9 |
14 |
95349694 |
- |
LOC107984710 |
NNNGGTGCACGCCATCACAC |
NGG |
2 |
0.3989 |
Tier III |
| 17 |
NC_000010.11 |
10 |
57350870 |
+ |
LOC105378313 |
NNNTGTGCATGTGATCACAC |
NGG |
2 |
0.3422 |
Tier III |
| 18 |
NC_000014.9 |
14 |
91529847 |
+ |
LOC101928957 |
NNNGGTGCACGTAATCACAC |
NTG |
2 |
0.036 |
Tier III |
| 19 |
NC_000019.10 |
19 |
8013095 |
+ |
LOC105372266 |
NNNGGTGCACGTGATCACAT |
NTG |
2 |
0.0117 |
Tier III |
| 20 |
NC_000007.14 |
7 |
3147609 |
+ |
LOC100129603 |
NNNGGTGCACGTGACCACAC |
NTG |
2 |
0.0106 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
155791703 |
+ |
Ankrd65 |
NNNGGTGCACCTGACCACAC |
NGG |
2 |
0.1169 |
Tier I |
| 2 |
NC_000081.6 |
15 |
79319336 |
- |
Pla2g6 |
NNNGGTGCACATGAACACAC |
NGG |
2 |
0.5789 |
Tier II |
| 3 |
NC_000069.6 |
3 |
131116296 |
+ |
Lef1 |
NNNTGTACACGTGATCACAC |
NGG |
2 |
0.3636 |
Tier II |
| 4 |
NC_000087.7 |
Y |
837939 |
+ |
Uba1y |
NNNGGTGTACTTGATCACAC |
NGG |
2 |
0.3365 |
Tier II |
| 5 |
NC_000076.6 |
10 |
80574449 |
+ |
Klf16 |
NNNGGTGTACGTGACCACAC |
NGG |
2 |
0.2386 |
Tier II |
| 6 |
NC_000073.6 |
7 |
98068160 |
+ |
Myo7a |
NNNGGTGCACGTGACCACAG |
NGG |
2 |
0.016 |
Tier II |
| 7 |
NC_000081.6 |
15 |
79319336 |
- |
Gm20420 |
NNNGGTGCACATGAACACAC |
NGG |
2 |
0.5789 |
Tier III |
| 8 |
NC_000071.6 |
5 |
73348787 |
+ |
Gm34496 |
NNNGGAGCACGTGTTCACAC |
NGG |
2 |
0.4622 |
Tier III |
| 9 |
NC_000087.7 |
Y |
675918 |
- |
Gm2098 |
NNNGGTGTACTTGATCACAC |
NGG |
2 |
0.3365 |
Tier III |
Other clones with same target sequence:
(none)
