Construct: sgRNA BRDN0001148443

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGGGCAGGGCTATCTTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80246

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000021.9 21 42579532 - SLC37A1 NNNGGCAGGGCTGTCTGATG NGG 2 0.0767 Tier I
2 NC_000022.11 22 42027320 + WBP2NL NNNGGAAGGGCAATCTTATG NGG 2 0.7429 Tier II
3 NC_000009.12 9 84959333 + NTRK2 NNNACCAGGGCTATCTTATG NGG 2 0.7071 Tier II
4 NC_000007.14 7 132980114 - CHCHD3 NNNGGCAGGGCTGTCTTATT NGG 2 0.4565 Tier II
5 NC_000011.10 11 9838965 - SBF2 NNNGGCAAGGCTTTCTTATG NGG 2 0.3 Tier II
6 NC_000019.10 19 5641438 + SAFB NNNGGCAGGGCTGTCTTGTG NGG 2 0.2609 Tier II
7 NC_000010.11 10 21825743 + DNAJC1 NNNTGCAGTGCTATCTTATG NGG 2 0.1939 Tier II
8 NC_000004.12 4 24796917 + SOD3 NNNGGCAGGGCTGTCTTAGG NGG 2 0.163 Tier II
9 NC_000001.11 1 154575502 + CHRNB2 NNNGGTAGGGCTATGTTATG NGG 2 0.0464 Tier II
10 NC_000009.12 9 72155619 + GDA NNNGGCAGGGCTATGTTATG NAG 2 0.013 Tier II
11 NC_000005.10 5 160304529 - CCNJL NNNGGCAGGGCTATCTTATG NTT 2 0.0 Tier II
12 NC_000016.10 16 59913800 - LINC02141 NNNAGAAGGGCTATCTTATG NGG 2 0.8357 Tier III
13 NC_000001.11 1 166186868 - LOC112268276 NNNGGAAGGGCTATCTTCTG NGG 2 0.1769 Tier III
14 NC_000010.11 10 64476008 + LOC124902439 NNNGGCAGGGCTCTCTTATG NGT 2 0.0034 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 80260162 + Ttll13 NNNGGCAGGGCTATCTTCTG NAG 2 0.0494 Tier I
2 NC_000084.6 18 66165800 + Ccbe1 NNNGGTAGGGCTGTCTTATG NGG 2 0.6056 Tier II
3 NC_000077.6 11 66408878 + Shisa6 NNNAGCAGGGCTATCTTATC NGG 2 0.3857 Tier II
4 NC_000073.6 7 24584204 - Zfp575 NNNGGCAGGGCTATCATGTG NGG 2 0.3636 Tier II
5 NC_000070.6 4 151284100 + Camta1 NNNGGCAGGGATATCTTATC NGG 2 0.3214 Tier II
6 NC_000070.6 4 35203844 - C9orf72 NNNGGCAGGGCAATCTTATG NAG 2 0.2074 Tier II
7 NC_000067.6 1 136421832 + Ddx59 NNNGGCAGGGCTATCTTGTC NGG 2 0.1714 Tier II
8 NC_000076.6 10 10435145 - Adgb NNNGGCAGGGCTGTATTATG NGG 2 0.1449 Tier II
9 NC_000084.6 18 80286628 + Slc66a2 NNNGGCGGGGCTATCTTCTG NGG 2 0.1345 Tier II
10 NC_000081.6 15 10309710 + Prlr NNNTGCAGGGCTTTCTTATG NGG 2 0.1091 Tier II
11 NC_000083.6 17 30225278 + Btbd9 NNNGGCAGGGCTATCTTGTG NAG 2 0.1037 Tier II
12 NC_000071.6 5 136983580 + Znhit1 NNNGGCAGGGATATTTTATG NGG 2 0.05 Tier II
13 NC_000075.6 9 97522832 - Clstn2 NNNGGCAGGGCTATCTGATG NAG 2 0.0305 Tier II
14 NC_000074.6 8 47056925 - Enpp6 NNNGGCAGGGCTATCTGATG NGA 2 0.0082 Tier II
15 NC_000076.6 10 41935586 - Armc2 NNNGGCAGGGCTAGCTTCTG NGG 2 0.0 Tier II
16 NC_000071.6 5 64386003 + Gm43837 NNNGGCTGGGCTATCTTCTG NGG 2 0.0833 Tier III
17 NC_000076.6 10 94612643 + Gm32898 NNNTGCAGGGCTATCTTCTG NGG 2 0.0693 Tier III
Download CSV

Other clones with same target sequence:

(none)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.