Construct: sgRNA BRDN0001148443
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGGGCAGGGCTATCTTATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80246
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
42579532 |
- |
SLC37A1 |
NNNGGCAGGGCTGTCTGATG |
NGG |
2 |
0.0767 |
Tier I |
| 2 |
NC_000022.11 |
22 |
42027320 |
+ |
WBP2NL |
NNNGGAAGGGCAATCTTATG |
NGG |
2 |
0.7429 |
Tier II |
| 3 |
NC_000009.12 |
9 |
84959333 |
+ |
NTRK2 |
NNNACCAGGGCTATCTTATG |
NGG |
2 |
0.7071 |
Tier II |
| 4 |
NC_000007.14 |
7 |
132980114 |
- |
CHCHD3 |
NNNGGCAGGGCTGTCTTATT |
NGG |
2 |
0.4565 |
Tier II |
| 5 |
NC_000011.10 |
11 |
9838965 |
- |
SBF2 |
NNNGGCAAGGCTTTCTTATG |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000019.10 |
19 |
5641438 |
+ |
SAFB |
NNNGGCAGGGCTGTCTTGTG |
NGG |
2 |
0.2609 |
Tier II |
| 7 |
NC_000010.11 |
10 |
21825743 |
+ |
DNAJC1 |
NNNTGCAGTGCTATCTTATG |
NGG |
2 |
0.1939 |
Tier II |
| 8 |
NC_000004.12 |
4 |
24796917 |
+ |
SOD3 |
NNNGGCAGGGCTGTCTTAGG |
NGG |
2 |
0.163 |
Tier II |
| 9 |
NC_000001.11 |
1 |
154575502 |
+ |
CHRNB2 |
NNNGGTAGGGCTATGTTATG |
NGG |
2 |
0.0464 |
Tier II |
| 10 |
NC_000009.12 |
9 |
72155619 |
+ |
GDA |
NNNGGCAGGGCTATGTTATG |
NAG |
2 |
0.013 |
Tier II |
| 11 |
NC_000005.10 |
5 |
160304529 |
- |
CCNJL |
NNNGGCAGGGCTATCTTATG |
NTT |
2 |
0.0 |
Tier II |
| 12 |
NC_000016.10 |
16 |
59913800 |
- |
LINC02141 |
NNNAGAAGGGCTATCTTATG |
NGG |
2 |
0.8357 |
Tier III |
| 13 |
NC_000001.11 |
1 |
166186868 |
- |
LOC112268276 |
NNNGGAAGGGCTATCTTCTG |
NGG |
2 |
0.1769 |
Tier III |
| 14 |
NC_000010.11 |
10 |
64476008 |
+ |
LOC124902439 |
NNNGGCAGGGCTCTCTTATG |
NGT |
2 |
0.0034 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
80260162 |
+ |
Ttll13 |
NNNGGCAGGGCTATCTTCTG |
NAG |
2 |
0.0494 |
Tier I |
| 2 |
NC_000084.6 |
18 |
66165800 |
+ |
Ccbe1 |
NNNGGTAGGGCTGTCTTATG |
NGG |
2 |
0.6056 |
Tier II |
| 3 |
NC_000077.6 |
11 |
66408878 |
+ |
Shisa6 |
NNNAGCAGGGCTATCTTATC |
NGG |
2 |
0.3857 |
Tier II |
| 4 |
NC_000073.6 |
7 |
24584204 |
- |
Zfp575 |
NNNGGCAGGGCTATCATGTG |
NGG |
2 |
0.3636 |
Tier II |
| 5 |
NC_000070.6 |
4 |
151284100 |
+ |
Camta1 |
NNNGGCAGGGATATCTTATC |
NGG |
2 |
0.3214 |
Tier II |
| 6 |
NC_000070.6 |
4 |
35203844 |
- |
C9orf72 |
NNNGGCAGGGCAATCTTATG |
NAG |
2 |
0.2074 |
Tier II |
| 7 |
NC_000067.6 |
1 |
136421832 |
+ |
Ddx59 |
NNNGGCAGGGCTATCTTGTC |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000076.6 |
10 |
10435145 |
- |
Adgb |
NNNGGCAGGGCTGTATTATG |
NGG |
2 |
0.1449 |
Tier II |
| 9 |
NC_000084.6 |
18 |
80286628 |
+ |
Slc66a2 |
NNNGGCGGGGCTATCTTCTG |
NGG |
2 |
0.1345 |
Tier II |
| 10 |
NC_000081.6 |
15 |
10309710 |
+ |
Prlr |
NNNTGCAGGGCTTTCTTATG |
NGG |
2 |
0.1091 |
Tier II |
| 11 |
NC_000083.6 |
17 |
30225278 |
+ |
Btbd9 |
NNNGGCAGGGCTATCTTGTG |
NAG |
2 |
0.1037 |
Tier II |
| 12 |
NC_000071.6 |
5 |
136983580 |
+ |
Znhit1 |
NNNGGCAGGGATATTTTATG |
NGG |
2 |
0.05 |
Tier II |
| 13 |
NC_000075.6 |
9 |
97522832 |
- |
Clstn2 |
NNNGGCAGGGCTATCTGATG |
NAG |
2 |
0.0305 |
Tier II |
| 14 |
NC_000074.6 |
8 |
47056925 |
- |
Enpp6 |
NNNGGCAGGGCTATCTGATG |
NGA |
2 |
0.0082 |
Tier II |
| 15 |
NC_000076.6 |
10 |
41935586 |
- |
Armc2 |
NNNGGCAGGGCTAGCTTCTG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000071.6 |
5 |
64386003 |
+ |
Gm43837 |
NNNGGCTGGGCTATCTTCTG |
NGG |
2 |
0.0833 |
Tier III |
| 17 |
NC_000076.6 |
10 |
94612643 |
+ |
Gm32898 |
NNNTGCAGGGCTATCTTCTG |
NGG |
2 |
0.0693 |
Tier III |
Other clones with same target sequence:
(none)
