Construct: sgRNA BRDN0001148444
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGAAGCGAATCCGGAAGCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIPK1 (8737)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76533
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
3085390 |
+ |
RIPK1 |
NNNAAGCGAATCCGGAAGCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
57059486 |
- |
NLRC5 |
NNNAAGTGAATCCGGAAGCT |
NTG |
2 |
0.0317 |
Tier I |
| 3 |
NC_000003.12 |
3 |
73158446 |
- |
LOC107986098 |
NNNAAGCTATTCCGGAAGCT |
NGG |
2 |
0.5515 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
3692103 |
+ |
Dtnb |
NNNAAGCATATCCGGAAGCT |
NGG |
2 |
0.6 |
Tier II |
| 2 |
NC_000076.6 |
10 |
89234944 |
+ |
Ano4 |
NNNAAGAGAATCCAGAAGCT |
NGG |
2 |
0.5625 |
Tier II |
| 3 |
NC_000072.6 |
6 |
88798975 |
- |
Mgll |
NNNAAGGGAATACGGAAGCT |
NGG |
2 |
0.3361 |
Tier II |
| 4 |
NC_000068.7 |
2 |
80012051 |
- |
Pde1a |
NNNAAGTGAATCAGGAAGCT |
NGG |
2 |
0.3125 |
Tier II |
| 5 |
NC_000070.6 |
4 |
148140151 |
- |
Mad2l2 |
NNNAAGCGAATCCGCAGGCT |
NGG |
2 |
0.0481 |
Tier II |
Other clones with same target sequence:
(none)
