Construct: sgRNA BRDN0001148451

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTAGGATACCATGGCCAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK4 (6789)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76076

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 44995102 + STK4 NNNAGGATACCATGGCCAAG NGG 0 1.0 Tier I
2 NC_000016.10 16 11276280 - PRM2 NNNAGGACACCACGGCCAAG NGG 2 0.7895 Tier I
3 NC_000003.12 3 43459559 + ANO10 NNNAGGAGAACATGGCCAAG NGG 2 0.6356 Tier II
4 NC_000008.11 8 142322055 + TSNARE1 NNNAGTATACCATGACCAAG NGG 2 0.6275 Tier II
5 NC_000007.14 7 101525993 - COL26A1 NNNAAGATACCATGGCCAAT NGG 2 0.6067 Tier II
6 NC_000015.10 15 100453308 + CERS3 NNNAACATACCATGGCCAAG NGG 2 0.5909 Tier II
7 NC_000003.12 3 41440514 - ULK4 NNNAATATACCATGGCCAAG NGG 2 0.5778 Tier II
8 NC_000001.11 1 156104941 + LMNA NNNAGGGGACCATGGCCAAG NGG 2 0.5176 Tier II
9 NC_000008.11 8 11772493 - NEIL2 NNNAGGATGCAATGGCCAAG NGG 2 0.5 Tier II
10 NC_000001.11 1 57257140 - DAB1 NNNTGGAGACCATGGCCAAG NGG 2 0.4667 Tier II
11 NC_000002.12 2 1866198 + MYT1L NNNTGGAGACCATGGCCAAG NGG 2 0.4667 Tier II
12 NC_000002.12 2 119922540 - PTPN4 NNNAGGATATCATGGCTAAG NGG 2 0.4392 Tier II
13 NC_000004.12 4 2330684 + ZFYVE28 NNNAGGACAGCATGGCCAAG NGG 2 0.3889 Tier II
14 NC_000009.12 9 129501803 + LOC124900275 NNNAGGAGACCATGGCCTAG NGG 2 0.3667 Tier II
15 NC_000003.12 3 38294437 + SLC22A14 NNNAGGATATCATGGCCAGG NGG 2 0.3529 Tier II
16 NC_000003.12 3 87943814 + HTR1F NNNAGGATATCATGGCCAGG NGG 2 0.3529 Tier II
17 NC_000010.11 10 94838909 + CYP2C19 NNNAGGATATCATGGCCAGG NGG 2 0.3529 Tier II
18 NC_000011.10 11 77862836 + AAMDC NNNAGGATATCATGGCCAGG NGG 2 0.3529 Tier II
19 NC_000011.10 11 77862836 + RSF1 NNNAGGATATCATGGCCAGG NGG 2 0.3529 Tier II
20 NC_000010.11 10 69452483 - TSPAN15 NNNAGGAAACCATGGCCGAG NGG 2 0.32 Tier II
21 NC_000023.11 X 124814065 + TENM1 NNNAGGATAGAATGGCCAAG NGG 2 0.2917 Tier II
22 NC_000020.11 20 47071787 - EYA2 NNNAGGGTACCATGGCCAGG NGG 2 0.2647 Tier II
23 NC_000001.11 1 17694312 + ARHGEF10L NNNAGGACACCATGGCCCAG NGG 2 0.1905 Tier II
24 NC_000008.11 8 22759662 + PEBP4 NNNAGGATACCCAGGCCAAG NGG 2 0.1822 Tier II
25 NC_000019.10 19 56576532 + ZNF470 NNNAGTATACCATGGCCAAG NAG 2 0.1728 Tier II
26 NC_000021.9 21 42130775 + UMODL1 NNNAGGACACCATGGGCAAG NGG 2 0.1538 Tier II
27 NC_000003.12 3 123505806 + HACD2 NNNAGGATACCATGAGCAAG NGG 2 0.1448 Tier II
28 NC_000004.12 4 73227081 + ANKRD17 NNNAGGATACCATGGGCAAA NGG 2 0.1442 Tier II
29 NC_000001.11 1 207540156 - CR1 NNNAGGATACCATTTCCAAG NGG 2 0.0381 Tier II
30 NC_000001.11 1 207558713 - CR1 NNNAGGATACCATTTCCAAG NGG 2 0.0381 Tier II
31 NC_000001.11 1 207575770 - CR1 NNNAGGATACCATTTCCAAG NGG 2 0.0381 Tier II
32 NC_000001.11 1 207708353 - CR1L NNNAGGATACCATTTCCAAG NGG 2 0.0381 Tier II
33 NC_000016.10 16 11276280 - LOC105371082 NNNAGGACACCACGGCCAAG NGG 2 0.7895 Tier III
34 NC_000021.9 21 31000277 - LOC105372776 NNNAGGATACAATGGCCAAG NGG 1 0.75 Tier III
35 NC_000007.14 7 115092619 - LINC01393 NNNAGGAAAACATGGCCAAG NGG 2 0.6933 Tier III
36 NC_000002.12 2 65498171 - LOC105374781 NNNAGGTTACCATGGCCAAG NGG 1 0.4375 Tier III
37 NC_000009.12 9 129501803 + LINC00963 NNNAGGAGACCATGGCCTAG NGG 2 0.3667 Tier III
38 NC_000004.12 4 82476431 - LOC105377310 NNNAGGATATCATGGCCAGG NGG 2 0.3529 Tier III
39 NC_000003.12 3 103630865 + TUBBP11 NNNAGGATGCCATGGCCAAG NAG 2 0.1728 Tier III
40 NC_000010.11 10 22454488 + LOC105376449 NNNAGGCTACTATGGCCAAG NGG 2 0.1346 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 108836542 + Celsr3 NNNAGGACACCATGGCCATG NGG 2 0.5385 Tier I
2 NC_000068.7 2 164096751 + Stk4 NNNAGGATACCATGGCAAAG NGG 1 0.4667 Tier I
3 NC_000068.7 2 180662081 - Dido1 NNNAGGATTCCAAGGCCAAG NGG 2 0.4154 Tier I
4 NC_000071.6 5 122764108 + Camkk2 NNNAGGATTCCATGCCCAAG NGG 2 0.1636 Tier I
5 NC_000077.6 11 29741752 + Rtn4 NNNAGGATGCCATGGCCAAG NGA 2 0.0463 Tier I
6 NC_000075.6 9 42062765 - Sorl1 NNNAGGACACCATGGCCAAA NGG 2 0.9375 Tier II
7 NC_000083.6 17 30732951 + Dnah8 NNNAAAATACCATGGCCAAG NGG 2 0.8667 Tier II
8 NC_000067.6 1 10310229 - Cpa6 NNNAGGACACCATGGCCAAT NGG 2 0.7 Tier II
9 NC_000069.6 3 145864964 + Ddah1 NNNAGGAAAACATGGCCAAG NGG 2 0.6933 Tier II
10 NC_000079.6 13 41020058 - Tmem14c NNNAGCACACCATGGCCAAG NGG 2 0.6818 Tier II
11 NC_000075.6 9 42060000 + Sorl1 NNNAGCATACCATGACCAAG NGG 2 0.6417 Tier II
12 NC_000069.6 3 144590199 + Selenof NNNTGGATACCATGGACAAG NGG 2 0.6364 Tier II
13 NC_000084.6 18 24213334 + Galnt1 NNNGGGATACCATGACCAAG NGG 2 0.5882 Tier II
14 NC_000068.7 2 140121108 - Esf1 NNNAGGAGACCAAGGCCAAG NGG 2 0.5077 Tier II
15 NC_000071.6 5 30849102 - Mapre3 NNNTGGATGCCATGGCCAAG NGG 2 0.4242 Tier II
16 NC_000079.6 13 94142066 + Lhfpl2 NNNAGAATAGCATGGCCAAG NGG 2 0.3889 Tier II
17 NC_000082.6 16 11600811 + Snx29 NNNAGGATTCCATGGCCAAC NGG 2 0.2571 Tier II
18 NC_000069.6 3 118795170 - Dpyd NNNAGGATGCCATGCCCAAG NGG 2 0.1818 Tier II
19 NC_000073.6 7 82770761 + Efl1 NNNAGGATGCCATGCCCAAG NGG 2 0.1818 Tier II
20 NC_000076.6 10 78058286 - Dnmt3l NNNAGCATACCATGGCCAAG NAG 2 0.1768 Tier II
21 NC_000067.6 1 60547135 + Raph1 NNNAGGAAACCATGGCCCAG NGG 2 0.1524 Tier II
22 NC_000075.6 9 49512738 - Ncam1 NNNAGGATACCATGTCCATG NGG 2 0.0769 Tier II
23 NC_000080.6 14 21611508 - Kat6b NNNAGGATACCATGGCCAAG NGA 1 0.0694 Tier II
24 NC_000083.6 17 55761578 - Adgre4 NNNAGGATTCCATGGCCAAG NTG 2 0.0234 Tier II
25 NC_000075.6 9 41818321 + Gm35940 NNNAGGTTACCATGGCCAAG NGG 1 0.4375 Tier III
26 NC_000069.6 3 73226221 - Gm20754 NNNAGGATACCAAGGCTAAG NGG 2 0.3231 Tier III
27 NC_000073.6 7 14605391 + Nlrp5-ps NNNAGGATACCAGGGCCAAG NAG 2 0.0676 Tier III
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Other clones with same target sequence:

(none)