Construct: sgRNA BRDN0001148452
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTGATGTGCATTAGCACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA3 (6197)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75943
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
20175266 |
+ |
RPS6KA3 |
NNNTGATGTGCATTAGCACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
71451597 |
+ |
THSD4 |
NNNTGATTTGCATTAACACT |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000005.10 |
5 |
171269134 |
+ |
RANBP17 |
NNNTGATTTGCATTAGCACT |
NGG |
1 |
0.625 |
Tier II |
| 4 |
NC_000012.12 |
12 |
108693935 |
- |
CORO1C |
NNNTGAAGTGCTTTAGCACT |
NGG |
2 |
0.2917 |
Tier II |
| 5 |
NC_000007.14 |
7 |
87028440 |
+ |
ELAPOR2 |
NNNTGATGTGCCCTAGCACT |
NGG |
2 |
0.2078 |
Tier II |
| 6 |
NC_000006.12 |
6 |
157598244 |
+ |
ZDHHC14 |
NNNTGGTGTGGATTAGCACT |
NGG |
2 |
0.1786 |
Tier II |
| 7 |
NC_000010.11 |
10 |
66253264 |
- |
CTNNA3 |
NNNTAATGTGCATTTGCACT |
NGG |
2 |
0.1733 |
Tier II |
| 8 |
NC_000014.9 |
14 |
68090238 |
+ |
RAD51B |
NNNTGATGTGCTTTAGCTCT |
NGG |
2 |
0.1667 |
Tier II |
| 9 |
NC_000001.11 |
1 |
42952103 |
+ |
SLC2A1 |
NNNTGATGGGCATTTGCACT |
NGG |
2 |
0.1238 |
Tier II |
| 10 |
NC_000004.12 |
4 |
172350822 |
+ |
GALNTL6 |
NNNTGATGTACATTAGCACT |
NGA |
2 |
0.0648 |
Tier II |
| 11 |
NC_000003.12 |
3 |
179759452 |
- |
USP13 |
NNNTGATGTGCATTAGCACA |
NTG |
2 |
0.0219 |
Tier II |
| 12 |
NC_000011.10 |
11 |
58070490 |
- |
OR9Q1 |
NNNTGATGTGCATGAGCATT |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000002.12 |
2 |
113378276 |
+ |
LINC02966 |
NNNTGATGGACATTAGCACT |
NGG |
2 |
0.5778 |
Tier III |
| 14 |
NC_000015.10 |
15 |
92478629 |
+ |
C15orf32 |
NNNTCATGTGCATCAGCACT |
NGG |
2 |
0.2245 |
Tier III |
| 15 |
NC_000010.11 |
10 |
66253264 |
- |
LOC124902441 |
NNNTAATGTGCATTTGCACT |
NGG |
2 |
0.1733 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
159337941 |
- |
Rps6ka3 |
NNNTGATGTGCGTTAGCACT |
NGG |
1 |
0.7222 |
Tier I |
| 2 |
NC_000080.6 |
14 |
18030467 |
- |
Thrb |
NNNTGATGTGAATTAGCACT |
NGG |
1 |
0.75 |
Tier II |
| 3 |
NC_000077.6 |
11 |
33240799 |
- |
Ranbp17 |
NNNTGATTTGCATTAGCACT |
NGG |
1 |
0.625 |
Tier II |
| 4 |
NC_000085.6 |
19 |
8594382 |
- |
Slc22a8 |
NNNTCTTGTGCATTAGCACT |
NGG |
2 |
0.5612 |
Tier II |
| 5 |
NC_000067.6 |
1 |
133719814 |
- |
Atp2b4 |
NNNTGATGTGCACCAGCACT |
NGG |
2 |
0.2256 |
Tier II |
| 6 |
NC_000083.6 |
17 |
30147360 |
+ |
Zfand3 |
NNNTGATGTGCTTTAGCACA |
NGG |
2 |
0.1875 |
Tier II |
| 7 |
NC_000084.6 |
18 |
43288179 |
+ |
Stk32a |
NNNTGGTGTGCAGTAGCACT |
NGG |
2 |
0.1863 |
Tier II |
| 8 |
NC_000069.6 |
3 |
137871349 |
+ |
Dnajb14 |
NNNTGATGTGGTTTAGCACT |
NGG |
2 |
0.0833 |
Tier II |
| 9 |
NC_000072.6 |
6 |
34083546 |
+ |
Lrguk |
NNNTGATGTGTATTTGCACT |
NGG |
2 |
0.0615 |
Tier II |
Other clones with same target sequence:
(none)
