Construct: sgRNA BRDN0001148465
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGACCAATAGAAGCAACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SPHK2 (56848)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77093
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
48627727 |
- |
SPHK2 |
NNNACCAATAGAAGCAACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
37999025 |
+ |
ZFAND3 |
NNNTCCAATAGAAGCAACCA |
NGG |
2 |
0.5966 |
Tier II |
| 3 |
NC_000022.11 |
22 |
42604732 |
+ |
POLDIP3 |
NNNACCAATAAAAGCAAACG |
NGG |
2 |
0.5385 |
Tier II |
| 4 |
NC_000023.11 |
X |
63695401 |
- |
ARHGEF9 |
NNNACCATTAGAAGCAACCT |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000023.11 |
X |
63696789 |
- |
ARHGEF9 |
NNNACCATTAGAAGCAACCT |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000002.12 |
2 |
124467859 |
- |
CNTNAP5 |
NNNACCATTAGAAGCAACAG |
NGG |
2 |
0.1837 |
Tier II |
| 7 |
NC_000007.14 |
7 |
21713916 |
- |
DNAH11 |
NNNACCAATAGAAGCATCCC |
NGG |
2 |
0.0571 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
11225605 |
+ |
Ms4a12 |
NNNACCAATAAAAGCAACAG |
NGG |
2 |
0.4286 |
Tier I |
| 2 |
NC_000085.6 |
19 |
27866666 |
+ |
Rfx3 |
NNNACCAATGGAAGCAACCA |
NGG |
2 |
0.5208 |
Tier II |
| 3 |
NC_000068.7 |
2 |
20501962 |
+ |
Etl4 |
NNNACCAAAAGAAGCAACTG |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000084.6 |
18 |
7557201 |
+ |
Mpp7 |
NNNACAAATAGAAGCAACCC |
NGG |
2 |
0.398 |
Tier II |
| 5 |
NC_000076.6 |
10 |
120854033 |
+ |
Msrb3 |
NNNTCCAAGAGAAGCAACCG |
NGG |
2 |
0.3939 |
Tier II |
| 6 |
NC_000079.6 |
13 |
93817060 |
- |
Arsb |
NNNAGCAAGAGAAGCAACCG |
NGG |
2 |
0.3714 |
Tier II |
| 7 |
NC_000078.6 |
12 |
56330549 |
+ |
Mbip |
NNNACCAAGAGAAGCAACAG |
NGG |
2 |
0.2653 |
Tier II |
| 8 |
NC_000083.6 |
17 |
29194985 |
- |
Cpne5 |
NNNACCAATAGAAGCCACCA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000085.6 |
19 |
11225605 |
+ |
4930526L06Rik |
NNNACCAATAAAAGCAACAG |
NGG |
2 |
0.4286 |
Tier III |
Other clones with same target sequence:
(none)
