Construct: sgRNA BRDN0001148469
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGTGCCGCCACCCCAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K2 (5871)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76781
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
64802594 |
- |
MAP4K2 |
NNNGTGCCGCCACCCCAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
76500402 |
- |
ESRRB |
NNNGTGCCTCCACCCAAATG |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000014.9 |
14 |
105367718 |
+ |
PACS2 |
NNNCTGCCGCCACCCCAGTG |
NGG |
2 |
0.2118 |
Tier II |
| 4 |
NC_000017.11 |
17 |
3861750 |
+ |
CAMKK1 |
NNNGTGCCACCACCCCAAGG |
NGG |
2 |
0.1607 |
Tier II |
| 5 |
NC_000002.12 |
2 |
241217580 |
- |
ANO7 |
NNNGTGCCGCCACCCCATCG |
NGG |
2 |
0.1379 |
Tier II |
| 6 |
NC_000005.10 |
5 |
140551213 |
+ |
SRA1 |
NNNGTGCTGCCAGCCCAATG |
NGG |
2 |
0.1193 |
Tier II |
| 7 |
NC_000007.14 |
7 |
5406323 |
- |
TNRC18 |
NNNGTGCCGCCACCACAATG |
NGC |
2 |
0.0049 |
Tier II |
| 8 |
NC_000008.11 |
8 |
37644065 |
+ |
LOC105379380 |
NNNGTGCCGCCACTCCACTG |
NGG |
2 |
0.1397 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6341804 |
+ |
Map4k2 |
NNNATGCCGCCACCCCAATG |
NCG |
2 |
0.0964 |
Tier I |
| 2 |
NC_000076.6 |
10 |
127041246 |
+ |
Eef1akmt3 |
NNNGTGCCGCCACCCCAAGG |
NGC |
2 |
0.0056 |
Tier I |
| 3 |
NC_000077.6 |
11 |
47091478 |
- |
Sgcd |
NNNGTGCCACCATCCCAATG |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000071.6 |
5 |
141247970 |
+ |
Sdk1 |
NNNGTGTCGCCACCCCAAAG |
NGG |
2 |
0.2321 |
Tier II |
| 5 |
NC_000083.6 |
17 |
66916934 |
+ |
Ptprm |
NNNGTGCCCCCTCCCCAATG |
NGG |
2 |
0.1795 |
Tier II |
| 6 |
NC_000082.6 |
16 |
34996891 |
- |
Mylk |
NNNGTGCAGCCACCCCCATG |
NGG |
2 |
0.1147 |
Tier II |
Other clones with same target sequence:
(none)
