Construct: sgRNA BRDN0001148474

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCACTAATGAGATTCTTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80247

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 95262128 + PPP1R9A NNNACTGATTAGATTCTTGT NGG 2 0.5735 Tier II
2 NC_000010.11 10 31834352 - ARHGAP12 NNNACTAATTAGATTCATGT NGG 2 0.4333 Tier II
3 NC_000006.12 6 57180885 - BAG2 NNNACTCAAGAGATTCTTGT NGG 2 0.4062 Tier II
4 NC_000001.11 1 74343544 - TNNI3K NNNGGTAATGAGATTCTTGT NGG 2 0.375 Tier II
5 NC_000001.11 1 74343544 - FPGT-TNNI3K NNNGGTAATGAGATTCTTGT NGG 2 0.375 Tier II
6 NC_000006.12 6 154054975 - OPRM1 NNNACTAATGAGATTCAAGT NGG 2 0.3556 Tier II
7 NC_000002.12 2 205786250 - NRP2 NNNACTAAGGAGATTCTTCT NGG 2 0.2775 Tier II
8 NC_000001.11 1 108693715 + PRPF38B NNNACTAATTAGATTTTTGT NGG 2 0.25 Tier II
9 NC_000005.10 5 102868731 - PAM NNNAATAATGATATTCTTGT NGG 2 0.2198 Tier II
10 NC_000015.10 15 58508706 - LIPC NNNACTAATGAGAACCTTGT NGG 2 0.1688 Tier II
11 NC_000008.11 8 117940584 - EXT1 NNNACTAATGAGTTTCTTGA NGG 2 0.1687 Tier II
12 NC_000010.11 10 50805314 + A1CF NNNACTAATGAGATTGATGT NGG 2 0.0821 Tier II
13 NC_000012.12 12 29701129 + TMTC1 NNNACTAATGAGATTATTGT NGA 2 0.0694 Tier II
14 NC_000004.12 4 154546933 - PLRG1 NNNAATAATGAGATTCTTGT NGC 2 0.0127 Tier II
15 NC_000002.12 2 193148061 - LOC107985969 NNNACTACTGGGATTCTTGT NGG 2 0.2786 Tier III
16 NC_000014.9 14 53711680 - LINC02331 NNNATTAATGAGATTTTTGT NGG 2 0.1958 Tier III
17 NC_000014.9 14 53711680 - LOC105370504 NNNATTAATGAGATTTTTGT NGG 2 0.1958 Tier III
18 NC_000005.10 5 124280734 + LINC01170 NNNAATAATGAGATTCTGGT NGG 2 0.1905 Tier III
19 NC_000011.10 11 127300622 - LINC02712 NNNACTCTTGAGATTCTTGT NGG 2 0.1875 Tier III
20 NC_000001.11 1 116410987 + ATP1A1-AS1 NNNACTCATCAGATTCTTGT NGG 2 0.175 Tier III
21 NC_000004.12 4 25225645 + LOC105374535 NNNACTAATGAAATTGTTGT NGG 2 0.1436 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 96155874 - Klhl1 NNNACAAATGAGATTCTTGA NGG 2 0.4875 Tier II
2 NC_000077.6 11 47310045 - Sgcd NNNACTTATGAGATTCTTGT NGG 1 0.4375 Tier II
3 NC_000070.6 4 77291001 - Ptprd NNNACTATTGAGATTCTAGT NGG 2 0.2857 Tier II
4 NC_000068.7 2 161693384 + Ptprt NNNACTAATAAGACTCTTGT NGG 2 0.2667 Tier II
5 NC_000080.6 14 119585573 + Hs6st3 NNNAATAATGCGATTCTTGT NGG 2 0.2286 Tier II
6 NC_000081.6 15 53191921 - Ext1 NNNACTAATGAGTTTCTTGA NGG 2 0.1687 Tier II
7 NC_000069.6 3 59178319 + Med12l NNNACTACTGATATTCTTGT NGG 2 0.1648 Tier II
8 NC_000069.6 3 154961435 + Tnni3k NNNAGTAATGAGATTCTTGT NAG 2 0.1556 Tier II
9 NC_000077.6 11 82429307 - Tmem132e NNNAGTAATGAGCTTCTTGT NGG 2 0.1263 Tier II
10 NC_000071.6 5 37483324 + Stk32b NNNACTAATGAGATTCTTTC NGG 2 0.0606 Tier II
11 NC_000085.6 19 34602592 + Ifit1bl1 NNNCCTAATGAGATTGTTGT NGG 2 0.0543 Tier II
12 NC_000073.6 7 50066807 - Nell1 NNNACTAATTAGATTCTTGT NTG 2 0.0317 Tier II
13 NC_000071.6 5 132418378 - Auts2 NNNACTAATGAGATTCTTGA NGT 2 0.0091 Tier II
14 NC_000074.6 8 37013699 + G630064G18Rik NNNATTATTGAGATTCTTGT NGG 2 0.2727 Tier III
15 NC_000078.6 12 117318849 + Gm5441 NNNACTAATGAGATTCTTCA NGG 2 0.2522 Tier III
16 NC_000078.6 12 111697123 - Gm51961 NNNACTAATGAGGTCCTTGT NGG 2 0.1779 Tier III
Download CSV

Other clones with same target sequence:

(none)

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