Construct: sgRNA BRDN0001148474
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCACTAATGAGATTCTTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80247
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
95262128 |
+ |
PPP1R9A |
NNNACTGATTAGATTCTTGT |
NGG |
2 |
0.5735 |
Tier II |
| 2 |
NC_000010.11 |
10 |
31834352 |
- |
ARHGAP12 |
NNNACTAATTAGATTCATGT |
NGG |
2 |
0.4333 |
Tier II |
| 3 |
NC_000006.12 |
6 |
57180885 |
- |
BAG2 |
NNNACTCAAGAGATTCTTGT |
NGG |
2 |
0.4062 |
Tier II |
| 4 |
NC_000001.11 |
1 |
74343544 |
- |
TNNI3K |
NNNGGTAATGAGATTCTTGT |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000001.11 |
1 |
74343544 |
- |
FPGT-TNNI3K |
NNNGGTAATGAGATTCTTGT |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000006.12 |
6 |
154054975 |
- |
OPRM1 |
NNNACTAATGAGATTCAAGT |
NGG |
2 |
0.3556 |
Tier II |
| 7 |
NC_000002.12 |
2 |
205786250 |
- |
NRP2 |
NNNACTAAGGAGATTCTTCT |
NGG |
2 |
0.2775 |
Tier II |
| 8 |
NC_000001.11 |
1 |
108693715 |
+ |
PRPF38B |
NNNACTAATTAGATTTTTGT |
NGG |
2 |
0.25 |
Tier II |
| 9 |
NC_000005.10 |
5 |
102868731 |
- |
PAM |
NNNAATAATGATATTCTTGT |
NGG |
2 |
0.2198 |
Tier II |
| 10 |
NC_000015.10 |
15 |
58508706 |
- |
LIPC |
NNNACTAATGAGAACCTTGT |
NGG |
2 |
0.1688 |
Tier II |
| 11 |
NC_000008.11 |
8 |
117940584 |
- |
EXT1 |
NNNACTAATGAGTTTCTTGA |
NGG |
2 |
0.1687 |
Tier II |
| 12 |
NC_000010.11 |
10 |
50805314 |
+ |
A1CF |
NNNACTAATGAGATTGATGT |
NGG |
2 |
0.0821 |
Tier II |
| 13 |
NC_000012.12 |
12 |
29701129 |
+ |
TMTC1 |
NNNACTAATGAGATTATTGT |
NGA |
2 |
0.0694 |
Tier II |
| 14 |
NC_000004.12 |
4 |
154546933 |
- |
PLRG1 |
NNNAATAATGAGATTCTTGT |
NGC |
2 |
0.0127 |
Tier II |
| 15 |
NC_000002.12 |
2 |
193148061 |
- |
LOC107985969 |
NNNACTACTGGGATTCTTGT |
NGG |
2 |
0.2786 |
Tier III |
| 16 |
NC_000014.9 |
14 |
53711680 |
- |
LINC02331 |
NNNATTAATGAGATTTTTGT |
NGG |
2 |
0.1958 |
Tier III |
| 17 |
NC_000014.9 |
14 |
53711680 |
- |
LOC105370504 |
NNNATTAATGAGATTTTTGT |
NGG |
2 |
0.1958 |
Tier III |
| 18 |
NC_000005.10 |
5 |
124280734 |
+ |
LINC01170 |
NNNAATAATGAGATTCTGGT |
NGG |
2 |
0.1905 |
Tier III |
| 19 |
NC_000011.10 |
11 |
127300622 |
- |
LINC02712 |
NNNACTCTTGAGATTCTTGT |
NGG |
2 |
0.1875 |
Tier III |
| 20 |
NC_000001.11 |
1 |
116410987 |
+ |
ATP1A1-AS1 |
NNNACTCATCAGATTCTTGT |
NGG |
2 |
0.175 |
Tier III |
| 21 |
NC_000004.12 |
4 |
25225645 |
+ |
LOC105374535 |
NNNACTAATGAAATTGTTGT |
NGG |
2 |
0.1436 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
96155874 |
- |
Klhl1 |
NNNACAAATGAGATTCTTGA |
NGG |
2 |
0.4875 |
Tier II |
| 2 |
NC_000077.6 |
11 |
47310045 |
- |
Sgcd |
NNNACTTATGAGATTCTTGT |
NGG |
1 |
0.4375 |
Tier II |
| 3 |
NC_000070.6 |
4 |
77291001 |
- |
Ptprd |
NNNACTATTGAGATTCTAGT |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000068.7 |
2 |
161693384 |
+ |
Ptprt |
NNNACTAATAAGACTCTTGT |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000080.6 |
14 |
119585573 |
+ |
Hs6st3 |
NNNAATAATGCGATTCTTGT |
NGG |
2 |
0.2286 |
Tier II |
| 6 |
NC_000081.6 |
15 |
53191921 |
- |
Ext1 |
NNNACTAATGAGTTTCTTGA |
NGG |
2 |
0.1687 |
Tier II |
| 7 |
NC_000069.6 |
3 |
59178319 |
+ |
Med12l |
NNNACTACTGATATTCTTGT |
NGG |
2 |
0.1648 |
Tier II |
| 8 |
NC_000069.6 |
3 |
154961435 |
+ |
Tnni3k |
NNNAGTAATGAGATTCTTGT |
NAG |
2 |
0.1556 |
Tier II |
| 9 |
NC_000077.6 |
11 |
82429307 |
- |
Tmem132e |
NNNAGTAATGAGCTTCTTGT |
NGG |
2 |
0.1263 |
Tier II |
| 10 |
NC_000071.6 |
5 |
37483324 |
+ |
Stk32b |
NNNACTAATGAGATTCTTTC |
NGG |
2 |
0.0606 |
Tier II |
| 11 |
NC_000085.6 |
19 |
34602592 |
+ |
Ifit1bl1 |
NNNCCTAATGAGATTGTTGT |
NGG |
2 |
0.0543 |
Tier II |
| 12 |
NC_000073.6 |
7 |
50066807 |
- |
Nell1 |
NNNACTAATTAGATTCTTGT |
NTG |
2 |
0.0317 |
Tier II |
| 13 |
NC_000071.6 |
5 |
132418378 |
- |
Auts2 |
NNNACTAATGAGATTCTTGA |
NGT |
2 |
0.0091 |
Tier II |
| 14 |
NC_000074.6 |
8 |
37013699 |
+ |
G630064G18Rik |
NNNATTATTGAGATTCTTGT |
NGG |
2 |
0.2727 |
Tier III |
| 15 |
NC_000078.6 |
12 |
117318849 |
+ |
Gm5441 |
NNNACTAATGAGATTCTTCA |
NGG |
2 |
0.2522 |
Tier III |
| 16 |
NC_000078.6 |
12 |
111697123 |
- |
Gm51961 |
NNNACTAATGAGGTCCTTGT |
NGG |
2 |
0.1779 |
Tier III |
Other clones with same target sequence:
(none)
