Construct: sgRNA BRDN0001148481
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTCACCATCAACACCCCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA1 (6195)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75499
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
26554728 |
- |
RPS6KA1 |
NNNCACCATCAACACCCCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
22331028 |
- |
CDH12 |
NNNCACCAACAACAACCCAT |
NGG |
2 |
0.2063 |
Tier II |
| 3 |
NC_000011.10 |
11 |
126872500 |
+ |
KIRREL3 |
NNNCACCATCATCTCCCCAT |
NGG |
2 |
0.1778 |
Tier II |
| 4 |
NC_000015.10 |
15 |
67194376 |
- |
SMAD3 |
NNNCACCATCACCTCCCCAT |
NGG |
2 |
0.1404 |
Tier II |
| 5 |
NC_000001.11 |
1 |
107381136 |
- |
NTNG1 |
NNNCACCATCAACACCACAT |
NAG |
2 |
0.121 |
Tier II |
| 6 |
NC_000007.14 |
7 |
74661279 |
- |
GTF2I |
NNNCACCATCAACACCACCT |
NGG |
2 |
0.0966 |
Tier II |
| 7 |
NC_000008.11 |
8 |
132415421 |
+ |
KCNQ3 |
NNNCACCATCACCACCCCAT |
NAG |
2 |
0.0682 |
Tier II |
| 8 |
NC_000012.12 |
12 |
103125406 |
+ |
C12orf42 |
NNNCACCATCAAGACTCCAT |
NGG |
2 |
0.042 |
Tier II |
| 9 |
NC_000016.10 |
16 |
58037647 |
+ |
MMP15 |
NNNCACCATCCACACCCCAC |
NGG |
2 |
0.0364 |
Tier II |
| 10 |
NC_000010.11 |
10 |
98796399 |
- |
HPSE2 |
NNNCACCATCAGCAGCCCAT |
NGG |
2 |
0.0361 |
Tier II |
| 11 |
NC_000019.10 |
19 |
10164077 |
+ |
DNMT1 |
NNNCACCATCAATACCCCAT |
NTG |
2 |
0.0273 |
Tier II |
| 12 |
NC_000019.10 |
19 |
10166883 |
+ |
DNMT1 |
NNNCACCATCAATACCCCAT |
NTG |
2 |
0.0273 |
Tier II |
| 13 |
NC_000011.10 |
11 |
126872500 |
+ |
LOC105369559 |
NNNCACCATCATCTCCCCAT |
NGG |
2 |
0.1778 |
Tier III |
| 14 |
NC_000002.12 |
2 |
18483112 |
- |
LOC105373454 |
NNNCACCATCAACACCCTAG |
NGG |
2 |
0.1134 |
Tier III |
| 15 |
NC_000021.9 |
21 |
17764849 |
- |
LOC124900465 |
NNNCAGCATCAACACCCCCT |
NGG |
2 |
0.1034 |
Tier III |
| 16 |
NC_000001.11 |
1 |
232898504 |
- |
LOC101927711 |
NNNCACCCTCAACACCCCCT |
NGG |
2 |
0.0887 |
Tier III |
| 17 |
NC_000009.12 |
9 |
126132932 |
+ |
LOC105376274 |
NNNCACCATCCACATCCCAT |
NGG |
2 |
0.0267 |
Tier III |
| 18 |
NC_000003.12 |
3 |
128467454 |
- |
DNAJB8-AS1 |
NNNCACCATCAACACCCCAG |
NGT |
2 |
0.0028 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
133865774 |
+ |
Rps6ka1 |
NNNCACCATCAGCACCCCAT |
NGG |
1 |
0.7222 |
Tier I |
| 2 |
NC_000071.6 |
5 |
27666261 |
- |
Dpp6 |
NNNTCCCATCAACACCCCAT |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000080.6 |
14 |
60017248 |
- |
Atp8a2 |
NNNCACCCACAACACCCCAT |
NGG |
2 |
0.398 |
Tier II |
| 4 |
NC_000075.6 |
9 |
28689452 |
+ |
Opcml |
NNNCACTTTCAACACCCCAT |
NGG |
2 |
0.3482 |
Tier II |
| 5 |
NC_000073.6 |
7 |
134746603 |
- |
Dock1 |
NNNCACCACCACCACCCCAT |
NGG |
2 |
0.2429 |
Tier II |
| 6 |
NC_000077.6 |
11 |
77823187 |
- |
Myo18a |
NNNCACCCTCATCACCCCAT |
NGG |
2 |
0.1429 |
Tier II |
| 7 |
NC_000084.6 |
18 |
6628019 |
- |
4921524L21Rik |
NNNCACCATCAGCACCCCAG |
NGG |
2 |
0.1275 |
Tier II |
| 8 |
NC_000068.7 |
2 |
143833150 |
- |
Bfsp1 |
NNNCACCATCAGCATCCCAT |
NGG |
2 |
0.0481 |
Tier II |
| 9 |
NC_000078.6 |
12 |
90138651 |
- |
Nrxn3 |
NNNCACCATCAACATCCAAT |
NGG |
2 |
0.0359 |
Tier II |
| 10 |
NC_000070.6 |
4 |
130934420 |
- |
Laptm5 |
NNNCACCATCAAGAACCCAT |
NGG |
2 |
0.0303 |
Tier II |
| 11 |
NC_000085.6 |
19 |
4549007 |
+ |
Pcx |
NNNCACCATCACCACCCCAT |
NGT |
2 |
0.0042 |
Tier II |
| 12 |
NC_000072.6 |
6 |
103738051 |
+ |
Gm52914 |
NNNCACCACCCACACCCCAT |
NGG |
2 |
0.3692 |
Tier III |
| 13 |
NC_000078.6 |
12 |
54132278 |
+ |
Gm35818 |
NNNCACCAACAAAACCCCAT |
NGG |
2 |
0.3571 |
Tier III |
| 14 |
NC_000069.6 |
3 |
100536528 |
- |
Gm40106 |
NNNCTCCATCAATACCCCAT |
NGG |
2 |
0.2545 |
Tier III |
| 15 |
NC_000082.6 |
16 |
49425067 |
- |
Gm6931 |
NNNCACCAGCAACACCCCAT |
NCG |
2 |
0.0663 |
Tier III |
Other clones with same target sequence:
(none)
