Construct: sgRNA BRDN0001148483
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAGGATTACCCATTGGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- VRK1 (7443)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75523
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
96833603 |
+ |
VRK1 |
NNNGGATTACCCATTGGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
102412301 |
+ |
PSD |
NNNGGATTACCCAATGACCA |
NGG |
2 |
0.5778 |
Tier II |
| 3 |
NC_000012.12 |
12 |
112346152 |
+ |
HECTD4 |
NNNGGTTAACCCATTGGCCA |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000006.12 |
6 |
160369934 |
+ |
SLC22A3 |
NNNGGATGACCCAATGGCCA |
NGG |
2 |
0.454 |
Tier II |
| 5 |
NC_000018.10 |
18 |
27154589 |
+ |
CHST9 |
NNNAGATTAGCCATTGGCCA |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000002.12 |
2 |
95331781 |
+ |
KCNIP3 |
NNNGGAATACCCACTGGCCA |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000001.11 |
1 |
174317843 |
+ |
RABGAP1L |
NNNGGATTTCCCTTTGGCCA |
NGG |
2 |
0.18 |
Tier II |
| 8 |
NC_000001.11 |
1 |
14549661 |
- |
KAZN |
NNNGGATTACCTATTGTCCA |
NGG |
2 |
0.1346 |
Tier II |
| 9 |
NC_000002.12 |
2 |
3261566 |
- |
EIPR1 |
NNNGGATTACCCTTTGCCCA |
NGG |
2 |
0.0706 |
Tier II |
| 10 |
NC_000017.11 |
17 |
38806229 |
+ |
CWC25 |
NNNGGATTATCCATTTGCCA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000006.12 |
6 |
160369934 |
+ |
LOC124901453 |
NNNGGATGACCCAATGGCCA |
NGG |
2 |
0.454 |
Tier III |
| 12 |
NC_000011.10 |
11 |
115734471 |
- |
LOC101928985 |
NNNGGATTACCCATTGGCTC |
NGG |
2 |
0.1049 |
Tier III |
| 13 |
NC_000011.10 |
11 |
115734471 |
- |
LINC02698 |
NNNGGATTACCCATTGGCTC |
NGG |
2 |
0.1049 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
106036676 |
+ |
Vrk1 |
NNNGGATTGCCCATTGGCCA |
NGG |
1 |
0.6667 |
Tier I |
| 2 |
NC_000076.6 |
10 |
86368057 |
- |
Syn3 |
NNNGGGTTTCCCATTGGCCA |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000084.6 |
18 |
52626628 |
- |
Zfp474 |
NNNGGAGTTCCCATTGGCCA |
NGG |
2 |
0.3529 |
Tier II |
| 4 |
NC_000076.6 |
10 |
94160143 |
- |
Nr2c1 |
NNNGGCTTACCCATAGGCCA |
NGG |
2 |
0.2632 |
Tier II |
| 5 |
NC_000079.6 |
13 |
83615358 |
+ |
Mef2c |
NNNGGATTTCCCATTGGGCA |
NGG |
2 |
0.08 |
Tier II |
| 6 |
NC_000083.6 |
17 |
86410225 |
+ |
Prkce |
NNNGGATTACCCAGTGGCCA |
NAG |
2 |
0.0 |
Tier II |
| 7 |
NC_000085.6 |
19 |
26802161 |
+ |
4931403E22Rik |
NNNGGATTGCCTATTGGCCA |
NGG |
2 |
0.359 |
Tier III |
| 8 |
NC_000085.6 |
19 |
26802161 |
+ |
Gm18380 |
NNNGGATTGCCTATTGGCCA |
NGG |
2 |
0.359 |
Tier III |
| 9 |
NC_000079.6 |
13 |
83615358 |
+ |
Gm52043 |
NNNGGATTTCCCATTGGGCA |
NGG |
2 |
0.08 |
Tier III |
| 10 |
NC_000075.6 |
9 |
68472804 |
- |
4930502A04Rik |
NNNGGATCACCCATGGGCCA |
NGG |
2 |
0.05 |
Tier III |
Other clones with same target sequence:
(none)
