Construct: sgRNA BRDN0001148483

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTAGGATTACCCATTGGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VRK1 (7443)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75523

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 96833603 + VRK1 NNNGGATTACCCATTGGCCA NGG 0 1.0 Tier I
2 NC_000010.11 10 102412301 + PSD NNNGGATTACCCAATGACCA NGG 2 0.5778 Tier II
3 NC_000012.12 12 112346152 + HECTD4 NNNGGTTAACCCATTGGCCA NGG 2 0.5714 Tier II
4 NC_000006.12 6 160369934 + SLC22A3 NNNGGATGACCCAATGGCCA NGG 2 0.454 Tier II
5 NC_000018.10 18 27154589 + CHST9 NNNAGATTAGCCATTGGCCA NGG 2 0.35 Tier II
6 NC_000002.12 2 95331781 + KCNIP3 NNNGGAATACCCACTGGCCA NGG 2 0.25 Tier II
7 NC_000001.11 1 174317843 + RABGAP1L NNNGGATTTCCCTTTGGCCA NGG 2 0.18 Tier II
8 NC_000001.11 1 14549661 - KAZN NNNGGATTACCTATTGTCCA NGG 2 0.1346 Tier II
9 NC_000002.12 2 3261566 - EIPR1 NNNGGATTACCCTTTGCCCA NGG 2 0.0706 Tier II
10 NC_000017.11 17 38806229 + CWC25 NNNGGATTATCCATTTGCCA NGG 2 0.0 Tier II
11 NC_000006.12 6 160369934 + LOC124901453 NNNGGATGACCCAATGGCCA NGG 2 0.454 Tier III
12 NC_000011.10 11 115734471 - LOC101928985 NNNGGATTACCCATTGGCTC NGG 2 0.1049 Tier III
13 NC_000011.10 11 115734471 - LINC02698 NNNGGATTACCCATTGGCTC NGG 2 0.1049 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 106036676 + Vrk1 NNNGGATTGCCCATTGGCCA NGG 1 0.6667 Tier I
2 NC_000076.6 10 86368057 - Syn3 NNNGGGTTTCCCATTGGCCA NGG 2 0.4286 Tier II
3 NC_000084.6 18 52626628 - Zfp474 NNNGGAGTTCCCATTGGCCA NGG 2 0.3529 Tier II
4 NC_000076.6 10 94160143 - Nr2c1 NNNGGCTTACCCATAGGCCA NGG 2 0.2632 Tier II
5 NC_000079.6 13 83615358 + Mef2c NNNGGATTTCCCATTGGGCA NGG 2 0.08 Tier II
6 NC_000083.6 17 86410225 + Prkce NNNGGATTACCCAGTGGCCA NAG 2 0.0 Tier II
7 NC_000085.6 19 26802161 + 4931403E22Rik NNNGGATTGCCTATTGGCCA NGG 2 0.359 Tier III
8 NC_000085.6 19 26802161 + Gm18380 NNNGGATTGCCTATTGGCCA NGG 2 0.359 Tier III
9 NC_000079.6 13 83615358 + Gm52043 NNNGGATTTCCCATTGGGCA NGG 2 0.08 Tier III
10 NC_000075.6 9 68472804 - 4930502A04Rik NNNGGATCACCCATGGGCCA NGG 2 0.05 Tier III
Download CSV

Other clones with same target sequence:

(none)

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