Construct: sgRNA BRDN0001148485

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTGCAGATAAAAGTGCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK3 (5063)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75680

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 111152420 + PAK3 NNNGCAGATAAAAGTGCACA NGG 0 1.0 Tier I
2 NC_000017.11 17 8880993 - PIK3R5 NNNGCAGATCAAAGTGGACA NGG 2 0.0196 Tier I
3 NC_000006.12 6 65229910 + EYS NNNGCAGAATAAAGTGCACA NGG 2 0.8193 Tier II
4 NC_000007.14 7 47484227 + TNS3 NNNGCAAATAAAAATGCACA NGG 2 0.75 Tier II
5 NC_000008.11 8 54569333 + RP1 NNNGTAGATAAAAGTACACA NGG 2 0.6364 Tier II
6 NC_000014.9 14 68879229 + ACTN1 NNNGGAAATAAAAGTGCACA NGG 2 0.6 Tier II
7 NC_000010.11 10 104983599 - SORCS3 NNNGCAGATGAAAGTACACA NGG 2 0.5556 Tier II
8 NC_000005.10 5 167886716 - TENM2 NNNGAAGAAAAAAGTGCACA NGG 2 0.5306 Tier II
9 NC_000008.11 8 94941044 + TP53INP1 NNNGGAGATTAAAGTGCACA NGG 2 0.5294 Tier II
10 NC_000008.11 8 94941044 + NDUFAF6 NNNGGAGATTAAAGTGCACA NGG 2 0.5294 Tier II
11 NC_000008.11 8 50334238 + SNTG1 NNNGAAGACAAAAGTGCACA NGG 2 0.5275 Tier II
12 NC_000012.12 12 40260466 + LRRK2 NNNGCAGAAGAAAGTGCACA NGG 2 0.5159 Tier II
13 NC_000002.12 2 231766466 - PDE6D NNNGCAAATAAAAGTGCAAA NGG 2 0.4286 Tier II
14 NC_000023.11 X 86751073 + DACH2 NNNGCAACTAAAAGTGCACA NGG 2 0.4286 Tier II
15 NC_000017.11 17 9342737 + STX8 NNNGCAGATAAAAGTGCAAA NGG 1 0.4286 Tier II
16 NC_000012.12 12 119861205 - CIT NNNGCAGCTAAAAGTGCACA NGG 1 0.4286 Tier II
17 NC_000014.9 14 93961714 + ASB2 NNNACAGATAAAAGTGAACA NGG 2 0.42 Tier II
18 NC_000022.11 22 44805510 - ARHGAP8 NNNGCACATAAAAGAGCACA NGG 2 0.398 Tier II
19 NC_000022.11 22 44805510 - PRR5-ARHGAP8 NNNGCACATAAAAGAGCACA NGG 2 0.398 Tier II
20 NC_000014.9 14 62737311 + KCNH5 NNNGCACATGAAAGTGCACA NGG 2 0.3819 Tier II
21 NC_000001.11 1 86090200 + COL24A1 NNNGAAGATAAAACTGCACA NGG 2 0.2449 Tier II
22 NC_000001.11 1 216959065 - ESRRG NNNGAAGATAAAAGTGCAAA NGG 2 0.2449 Tier II
23 NC_000019.10 19 14131962 + ASF1B NNNGAAGATAAAAGTGCAAA NGG 2 0.2449 Tier II
24 NC_000019.10 19 11028214 - SMARCA4 NNNCCAGCTAAAAGTGCACA NGG 2 0.2269 Tier II
25 NC_000020.11 20 31541086 + HM13 NNNGCAGCTAAAACTGCACA NGG 2 0.1837 Tier II
26 NC_000016.10 16 19605902 - VPS35L NNNGGAGATAACAGTGCACA NGG 2 0.1579 Tier II
27 NC_000010.11 10 37829664 - ZNF248 NNNGAAGATAACAGTGCACA NGG 2 0.1504 Tier II
28 NC_000001.11 1 116648476 - IGSF3 NNNGCAGGTAAAAGTGCAGA NGG 2 0.0917 Tier II
29 NC_000003.12 3 126300832 + KLF15 NNNGCAGATACAAGTGCACC NGG 2 0.0909 Tier II
30 NC_000019.10 19 30490130 + ZNF536 NNNGCAGAGAAAAGTGCACA NGA 2 0.043 Tier II
31 NC_000013.11 13 110217106 + COL4A1 NNNCCAGATAAAAGTGCACA NGA 2 0.0368 Tier II
32 NC_000010.11 10 115706533 - ATRNL1 NNNGCAGATAAAAGTGCACT NTG 2 0.0234 Tier II
33 NC_000001.11 1 212081308 + DTL NNNGCAGATAAAAGTACACA NGC 2 0.0222 Tier II
34 NC_000002.12 2 2976275 + LINC01250 NNNGCAAAGAAAAGTGCACA NGG 2 0.619 Tier III
35 NC_000009.12 9 41722864 + LOC107987000 NNNGCAGAAAAAGGTGCACA NGG 2 0.6056 Tier III
36 NC_000009.12 9 61934919 + LOC107987007 NNNGCAGAAAAAGGTGCACA NGG 2 0.6056 Tier III
37 NC_000005.10 5 60527817 - PART1 NNNGCTGATAAAAGTGCTCA NGG 2 0.3571 Tier III
38 NC_000010.11 10 75430342 + LOC107984293 NNNGCAGACAAAAGTGCACA NTG 2 0.036 Tier III
39 NC_000017.11 17 8880993 - LOC124903919 NNNGCAGATCAAAGTGGACA NGG 2 0.0196 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 143716121 + Pak3 NNNTCAGATAAAAGTGCCCA NGG 2 0.0693 Tier I
2 NC_000067.6 1 38413408 + Aff3 NNNGTAGATAAAAGTACACA NGG 2 0.6364 Tier II
3 NC_000072.6 6 102550832 - Cntn3 NNNGAAGATAAAAGTGCACA NGG 1 0.5714 Tier II
4 NC_000072.6 6 57520919 - Ppm1k NNNACAGAGAAAAGTGCACA NGG 2 0.5571 Tier II
5 NC_000068.7 2 128670392 + Anapc1 NNNGGAGAAAAAAGTGCACA NGG 2 0.5571 Tier II
6 NC_000079.6 13 41148270 - Sycp2l NNNGCATATTAAAGTGCACA NGG 2 0.5042 Tier II
7 NC_000077.6 11 22920996 - Commd1 NNNGCAGATAAAGATGCACA NGG 2 0.4891 Tier II
8 NC_000073.6 7 50553998 + Nell1 NNNACAGATAAAAGTGCTCA NGG 2 0.45 Tier II
9 NC_000082.6 16 72143131 - Robo1 NNNGCAGACAAAAGTGTACA NGG 2 0.4308 Tier II
10 NC_000080.6 14 68620886 + Adam28 NNNGCAGATTAAACTGCACA NGG 2 0.3782 Tier II
11 NC_000082.6 16 90937379 + Synj1 NNNGCAGAGAAAAGAGCACA NGG 2 0.3584 Tier II
12 NC_000076.6 10 70099682 + Ccdc6 NNNGGAGATAAAAGAGCACA NGG 2 0.3474 Tier II
13 NC_000071.6 5 43251693 + Cpeb2 NNNGCAGAAAATAGTGCACA NGG 2 0.3095 Tier II
14 NC_000071.6 5 13515048 - Sema3a NNNGCAGAGAAAAGTGCAAA NGG 2 0.2653 Tier II
15 NC_000068.7 2 121248977 + Trp53bp1 NNNGCAGAAAAAAGTGCACA NAG 2 0.2407 Tier II
16 NC_000068.7 2 153814871 - Efcab8 NNNTCAGATAGAAGTGCACA NGG 2 0.2364 Tier II
17 NC_000084.6 18 73671269 - Smad4 NNNTGAGATAAAAGTGCACA NGG 2 0.2182 Tier II
18 NC_000084.6 18 80764154 + Atp9b NNNGCACATAAATGTGCACA NGG 2 0.2063 Tier II
19 NC_000082.6 16 5104288 + Ppl NNNGCAGATAAAAGTGCCCA NGG 1 0.1905 Tier II
20 NC_000084.6 18 42792644 - Ppp2r2b NNNGCAGATAAAAGAGCAGA NGG 2 0.0724 Tier II
21 NC_000083.6 17 30099646 + Zfand3 NNNGCACATAAAAGTGCACA NTG 2 0.0268 Tier II
22 NC_000079.6 13 69528263 + Tent4a NNNGCAGAGAAAAGTGCACA NTG 2 0.0241 Tier II
23 NC_000072.6 6 15260477 - Foxp2 NNNGCAGATAAATGTGCACA NGA 2 0.0208 Tier II
24 NC_000086.7 X 101868295 + Nhsl2 NNNGCAGATAAAAGTGCTCA NTG 2 0.0195 Tier II
25 NC_000083.6 17 80153918 + Galm NNNGCAGTTAAAAGTGCACA NTG 2 0.0167 Tier II
26 NC_000074.6 8 123030953 + Ankrd11 NNNGCAGATAAAAGTTCTCA NGG 2 0.0 Tier II
27 NC_000083.6 17 7281961 - Rps6ka2 NNNGCTGATAAAAGTCCACA NGG 2 0.0 Tier II
28 NC_000073.6 7 50553998 + Nell1os NNNACAGATAAAAGTGCTCA NGG 2 0.45 Tier III
29 NC_000076.6 10 70099682 + Gm51771 NNNGGAGATAAAAGAGCACA NGG 2 0.3474 Tier III
30 NC_000074.6 8 109096190 + D030068K23Rik NNNGCATATAAAATTGCACA NGG 2 0.1524 Tier III
31 NC_000081.6 15 5538480 + 5430437J10Rik NNNGCAGATAAAAGTGCCCA NAG 2 0.0494 Tier III
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Other clones with same target sequence:

(none)