Construct: sgRNA BRDN0001148486

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AATCTCATCAGTCTTTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME9 (347736)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76836

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 138306424 + NME9 NNNCTCATCAGTCTTTCCAT NGG 0 1.0 Tier I
2 NC_000003.12 3 52199216 + ALAS1 NNNTTCATCAGTCTTTCCAC NGG 2 0.0727 Tier I
3 NC_000010.11 10 62808406 - ADO NNNCTCATTAGTCTTTCCAA NGG 2 0.4922 Tier II
4 NC_000013.11 13 51145697 - C13orf42 NNNCTCATTAGTCTTTCCAA NGG 2 0.4922 Tier II
5 NC_000009.12 9 28056447 + LINGO2 NNNCTCAGCACTCTTTCCAT NGG 2 0.3143 Tier II
6 NC_000007.14 7 157585137 - PTPRN2 NNNCTCATCAGCCTTTCCAT NAG 2 0.2456 Tier II
7 NC_000017.11 17 41763624 + JUP NNNCTCATCAGCCTTTCCCT NGG 2 0.196 Tier II
8 NC_000001.11 1 212618827 + ATF3 NNNCTCAGCAGTCTTTCCAG NGG 2 0.1294 Tier II
9 NC_000016.10 16 57515725 + CCDC102A NNNCCCATCAGTCTGTCCAT NGG 2 0.05 Tier II
10 NC_000001.11 1 49060845 - AGBL4 NNNCTCCTCAGTCTTTCCAC NGG 2 0.0398 Tier II
11 NC_000022.11 22 29023775 + ZNRF3 NNNCTCATCAGTCTTTCCAA NTG 2 0.0219 Tier II
12 NC_000017.11 17 9298371 - STX8 NNNCTCATCAGCCTTTCCAT NGC 2 0.0211 Tier II
13 NC_000023.11 X 13658393 - TCEANC NNNCTCTTCAGTCTTTCCAT NTG 2 0.017 Tier II
14 NC_000012.12 12 1071684 + ERC1 NNNCTCATCACTCTTTCCAT NTG 2 0.0167 Tier II
15 NC_000022.11 22 26333936 - SEZ6L NNNCTCATCAGTCTTTCCAA NGC 2 0.0125 Tier II
16 NC_000003.12 3 73607515 + PDZRN3 NNNCTCATCAGTCTCTCCAT NTG 2 0.0106 Tier II
17 NC_000005.10 5 61450371 + ZSWIM6 NNNCTCATCAGTCTTTCCAT NCT 2 0.0 Tier II
18 NC_000023.11 X 153344639 - ZNF275 NNNCTCATCAGTCTTTCCAT NTA 2 0.0 Tier II
19 NC_000003.12 3 117520786 - LOC105374056 NNNCTAATCAGACTTTCCAT NGG 2 0.7429 Tier III
20 NC_000008.11 8 128513275 - LINC00824 NNNCTCAGCACTCTTTCCAT NGG 2 0.3143 Tier III
21 NC_000008.11 8 64043311 - LINC01414 NNNCTCTTCAGTCTTTCAAT NGG 2 0.2356 Tier III
22 NC_000007.14 7 156589978 - RNF32-DT NNNCGCATCAGTCTTTCCAG NGG 2 0.1129 Tier III
23 NC_000008.11 8 24179388 - LOC107986931 NNNCTCATCAGTCCTTCCAT NAG 2 0.0741 Tier III
24 NC_000001.11 1 38368080 + LOC105378657 NNNCTCATCAGTCTTGCCAG NGG 2 0.0611 Tier III
25 NC_000004.12 4 137074475 + LINC02511 NNNCTCCTCAGTCTTTCCAT NTG 2 0.017 Tier III
26 NC_000023.11 X 38919089 + LOC124905177 NNNTTCATCAGTCTTTCCAT NGT 2 0.0129 Tier III
27 NC_000009.12 9 89698577 - LINC03062 NNNCTCATCAGTCATTCCAT NGT 2 0.01 Tier III
28 NC_000009.12 9 89698577 - LOC105376140 NNNCTCATCAGTCATTCCAT NGT 2 0.01 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 100481914 - Cntnap5b NNNCTCATCTGTCTTTCCAA NGG 2 0.4963 Tier I
2 NC_000073.6 7 24399107 + Smg9 NNNCTCAGCTGTCTTTCCAT NGG 2 0.6471 Tier II
3 NC_000067.6 1 135574926 - Nav1 NNNCTCATCTGTCTTTCTAT NGG 2 0.5672 Tier II
4 NC_000073.6 7 58818787 + Atp10a NNNCTCATTAGTCTTTCCTT NGG 2 0.4712 Tier II
5 NC_000076.6 10 109696606 - Nav3 NNNCTCATCTGGCTTTCCAT NGG 2 0.4412 Tier II
6 NC_000067.6 1 24298319 + Col19a1 NNNCTCAACAGTCTTTCCTT NGG 2 0.4308 Tier II
7 NC_000084.6 18 42831278 - Ppp2r2b NNNCTCATCAGTCTTAACAT NGG 2 0.4242 Tier II
8 NC_000082.6 16 17366888 - Pi4ka NNNCTATTCAGTCTTTCCAT NGG 2 0.4062 Tier II
9 NC_000077.6 11 78359344 + Foxn1 NNNGTCATCAGACTTTCCAT NGG 2 0.4 Tier II
10 NC_000086.7 X 113450199 + Dach2 NNNTTCATCACTCTTTCCAT NGG 2 0.3429 Tier II
11 NC_000072.6 6 5938555 - Dync1i1 NNNCTCATGAGGCTTTCCAT NGG 2 0.3095 Tier II
12 NC_000083.6 17 49821802 + Kif6 NNNCTCATCACTCTTTCAAT NGG 2 0.2308 Tier II
13 NC_000081.6 15 66893351 - Ccn4 NNNCACATCATTCTTTCCAT NGG 2 0.1923 Tier II
14 NC_000074.6 8 17123777 + Csmd1 NNNCTCATCAGTCTTTCTAG NGG 2 0.1134 Tier II
15 NC_000069.6 3 156811097 - Negr1 NNNCTCATCAGTCTTTACCT NGG 2 0.0966 Tier II
16 NC_000077.6 11 31979285 + 4930524B15Rik NNNCTCTTCAGTCTTTCCAC NGG 2 0.0398 Tier II
17 NC_000080.6 14 29663130 + Cacna2d3 NNNCTCATCAGTCTTTGCAT NGA 2 0.0041 Tier II
18 NC_000067.6 1 10579888 + Cpa6 NNNCTCATCAGTCTTTCCAT NCT 2 0.0 Tier II
19 NC_000079.6 13 80960624 + Gm4211 NNNCTCATCAGTCTTACCAT NGG 1 0.9091 Tier III
20 NC_000070.6 4 142352896 + Gm46875 NNNCACATCTGTCTTTCCAT NGG 2 0.4412 Tier III
21 NC_000080.6 14 98944272 + Gm41228 NNNCTCATCAGTCTCTCCAT NGG 1 0.2727 Tier III
22 NC_000083.6 17 49821802 + Gm16555 NNNCTCATCACTCTTTCAAT NGG 2 0.2308 Tier III
23 NC_000074.6 8 34386357 - Gm33831 NNNCTCCTCAGTCTCTCCAT NGG 2 0.1193 Tier III
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Other clones with same target sequence:

(none)