Construct: sgRNA BRDN0001148493
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGAATACATCTCCACCCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MOK (5891)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75938
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
102250910 |
- |
MOK |
NNNAATACATCTCCACCCGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
110579118 |
- |
SVEP1 |
NNNATTACATCTCCACCCGC |
NGC |
2 |
0.0081 |
Tier I |
| 3 |
NC_000010.11 |
10 |
124542372 |
+ |
LHPP |
NNNAATACATCTCCACCTAC |
NGG |
2 |
0.4592 |
Tier II |
| 4 |
NC_000003.12 |
3 |
52276583 |
+ |
WDR82 |
NNNTATACATCTCCACCCTC |
NGG |
2 |
0.4242 |
Tier II |
| 5 |
NC_000002.12 |
2 |
233464601 |
- |
DGKD |
NNNGATACATCTCCACCCTC |
NGG |
2 |
0.4167 |
Tier II |
| 6 |
NC_000020.11 |
20 |
3705086 |
+ |
SIGLEC1 |
NNNAATACAACTCCACCCCC |
NGG |
2 |
0.3842 |
Tier II |
| 7 |
NC_000001.11 |
1 |
185813574 |
- |
HMCN1 |
NNNAATACAGCTCCACCTGC |
NGG |
2 |
0.3214 |
Tier II |
| 8 |
NC_000001.11 |
1 |
43550048 |
- |
PTPRF |
NNNAATCCACCTCCACCCGC |
NGG |
2 |
0.2333 |
Tier II |
| 9 |
NC_000014.9 |
14 |
106513153 |
- |
IGH |
NNNAATAGATCTACACCCGC |
NGG |
2 |
0.2473 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
85083510 |
- |
Man2b1 |
NNNAATACATTTCCGCCCGC |
NGG |
2 |
0.2 |
Tier II |
| 2 |
NC_000069.6 |
3 |
128378915 |
- |
Gm52639 |
NNNAAAACATCTCCACCCAC |
NGG |
2 |
0.619 |
Tier III |
Other clones with same target sequence:
(none)
