Construct: sgRNA BRDN0001148497

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGCTTCAGATAGATCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTBK2 (146057)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77971

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 42753039 - TTBK2 NNNCTTCAGATAGATCACAT NGG 0 1.0 Tier I
2 NC_000015.10 15 85118526 - PDE8A NNNTTTCAGAAAGATCACAT NGG 2 0.6 Tier II
3 NC_000005.10 5 35780012 + SPEF2 NNNATTCAGACAGATCACAT NGG 2 0.5614 Tier II
4 NC_000001.11 1 61817222 + PATJ NNNATTCAGATATATCACAT NGG 2 0.2526 Tier II
5 NC_000006.12 6 162589220 + PRKN NNNCTTGAGATAGTTCACAT NGG 2 0.251 Tier II
6 NC_000002.12 2 127267020 - ERCC3 NNNCTTAAGATTGATCACAT NGG 2 0.25 Tier II
7 NC_000008.11 8 104177593 + RIMS2 NNNCTTCAAATCGATCACAT NGG 2 0.1692 Tier II
8 NC_000005.10 5 9264167 + SEMA5A NNNCTTCAAATAGATCACAT NAG 2 0.1667 Tier II
9 NC_000012.12 12 45406260 + ANO6 NNNCTTCAGATACATCACAT NAG 2 0.1092 Tier II
10 NC_000010.11 10 69525899 - TSPAN15 NNNCTTCAGATACATCAGAT NGG 2 0.0561 Tier II
11 NC_000008.11 8 92057436 + RUNX1T1 NNNCTTCAAATAGATCACAT NGT 2 0.0104 Tier II
12 NC_000022.11 22 30048359 + HORMAD2-AS1 NNNCTTCAGAAAGATCACTT NGG 2 0.4038 Tier III
13 NC_000001.11 1 215003104 + LOC124904510 NNNCTTTAGATAGATCACAT NAG 2 0.2106 Tier III
14 NC_000001.11 1 166205889 + LOC112268276 NNNCTTTAGATAGATCACCT NGG 2 0.1681 Tier III
15 NC_000012.12 12 45406260 + LOC105369743 NNNCTTCAGATACATCACAT NAG 2 0.1092 Tier III
16 NC_000002.12 2 7424231 + LOC100506274 NNNCTTCAGATAGACCCCAT NGG 2 0.0481 Tier III
17 NC_000007.14 7 4436934 + LOC124901579 NNNCTTCAGACAGATCACAT NGA 2 0.0463 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 45028063 + Polr1e NNNCTTCAGAAAGGTCACAT NGG 2 0.35 Tier I
2 NC_000068.7 2 120746081 - Ttbk2 NNNCTTCAGATAGATCACAC NGG 1 0.0909 Tier I
3 NC_000069.6 3 153879403 + Msh4 NNNCTTCAGATAGATCAAAT NTG 2 0.021 Tier I
4 NC_000067.6 1 89582422 + Agap1 NNNCATCAGATAGATAACAT NGG 2 0.5 Tier II
5 NC_000084.6 18 13889633 - Zfp521 NNNCTTCAGTTAGATCACAA NGG 2 0.4963 Tier II
6 NC_000068.7 2 24841196 - Ehmt1 NNNCTTCAGATGGATCACTT NGG 2 0.3889 Tier II
7 NC_000070.6 4 151821172 - Camta1 NNNCTTCAGACAGAACACAT NGG 2 0.386 Tier II
8 NC_000071.6 5 130896591 + Galnt17 NNNGTTCAGATAGAACACAT NGG 2 0.2895 Tier II
9 NC_000072.6 6 8091075 - Col28a1 NNNCTTCAGTTAGACCACAT NGG 2 0.2406 Tier II
10 NC_000081.6 15 53168215 + Ext1 NNNCTCCAGATAGATCACAT NCG 2 0.0974 Tier II
11 NC_000072.6 6 62025278 + Ccser1 NNNATTCAGATAGATCACAT NGA 2 0.0585 Tier II
12 NC_000080.6 14 77682335 + Enox1 NNNCTTCAGATAGCTCACCT NGG 2 0.0443 Tier II
13 NC_000085.6 19 33580658 - Lipo3 NNNCTTCACATAGATCACAT NGA 2 0.0374 Tier II
14 NC_000085.6 19 33747032 - Lipo2 NNNCTTCACATAGATCACAT NGA 2 0.0374 Tier II
15 NC_000068.7 2 149936771 + Syndig1 NNNCTTCAGATAGATCAAAT NGA 2 0.0374 Tier II
16 NC_000071.6 5 34197077 - Zfyve28 NNNCTTCAGAGAGATCACAT NGA 2 0.0278 Tier II
17 NC_000072.6 6 137832814 - Dera NNNCTTCAGATAGATCACAC NAG 2 0.0236 Tier II
18 NC_000082.6 16 73390052 - 4930500H12Rik NNNCTTCAGATAAATCACAT NAG 2 0.2393 Tier III
19 NC_000085.6 19 33466073 - Lipo5 NNNCTTCACATAGATCACAT NGA 2 0.0374 Tier III
20 NC_000085.6 19 33680747 - Gm8980 NNNCTTCACATAGATCACAT NGA 2 0.0374 Tier III
21 NC_000075.6 9 27789336 + Gm39314 NNNCTTCAGATATATCACAT NGC 2 0.0067 Tier III
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Other clones with same target sequence:

(none)