Construct: sgRNA BRDN0001148499
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACTCACCAGTATACTACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCAKD (79877)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75873
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
45030102 |
+ |
DCAKD |
NNNTCACCAGTATACTACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
147688927 |
+ |
CNTNAP2 |
NNNACACCAGAATACTACCA |
NGG |
2 |
0.3571 |
Tier II |
| 3 |
NC_000002.12 |
2 |
102724051 |
+ |
MFSD9 |
NNNTCACCACTATACCACCA |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000001.11 |
1 |
93127850 |
+ |
MTF2 |
NNNTCACCACTATACTAACA |
NGG |
2 |
0.2154 |
Tier II |
| 5 |
NC_000012.12 |
12 |
122376384 |
- |
CLIP1 |
NNNTCACCACTATACTAACA |
NGG |
2 |
0.2154 |
Tier II |
| 6 |
NC_000013.11 |
13 |
36973117 |
- |
ALG5 |
NNNTCACCACTATACTAACA |
NGG |
2 |
0.2154 |
Tier II |
| 7 |
NC_000012.12 |
12 |
71477253 |
- |
LGR5 |
NNNTCACCAGTCTTCTACCA |
NGG |
2 |
0.1404 |
Tier II |
| 8 |
NC_000007.14 |
7 |
30319704 |
- |
ZNRF2 |
NNNTCACCAGTATCCTACTA |
NGG |
2 |
0.0989 |
Tier II |
| 9 |
NC_000008.11 |
8 |
22060809 |
- |
DMTN |
NNNTCACCAGGATACTACCC |
NGG |
2 |
0.0909 |
Tier II |
| 10 |
NC_000002.12 |
2 |
215405184 |
- |
FN1 |
NNNTCACCAGTATCCTACCA |
NGA |
2 |
0.0149 |
Tier II |
| 11 |
NC_000012.12 |
12 |
127651505 |
+ |
LOC105370068 |
NNNTCCCCAGTATACTAACA |
NGG |
2 |
0.2448 |
Tier III |
| 12 |
NC_000010.11 |
10 |
101255929 |
+ |
LINC02681 |
NNNTCACCACTATACTAACA |
NGG |
2 |
0.2154 |
Tier III |
| 13 |
NC_000005.10 |
5 |
16418477 |
- |
LINC02150 |
NNNTCACCAGCAGACTACCA |
NGG |
2 |
0.1739 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
102997363 |
+ |
Dcakd |
NNNTCACCAGTACACTACCA |
NTG |
2 |
0.0308 |
Tier I |
| 2 |
NC_000071.6 |
5 |
121366209 |
- |
Hectd4 |
NNNTCACAAGTATGCTACCA |
NGG |
2 |
0.3033 |
Tier II |
| 3 |
NC_000074.6 |
8 |
48610542 |
+ |
Tenm3 |
NNNTCACCAGCATACTACCA |
NAG |
2 |
0.1728 |
Tier II |
| 4 |
NC_000076.6 |
10 |
84697485 |
+ |
Polr3b |
NNNTCACCAGTATACTAGGA |
NGG |
2 |
0.0167 |
Tier II |
| 5 |
NC_000068.7 |
2 |
130981553 |
+ |
Atrn |
NNNTCACCACTATACTACCA |
NTG |
2 |
0.0156 |
Tier II |
| 6 |
NC_000076.6 |
10 |
67404775 |
+ |
Gm31962 |
NNNTCACCTATATACTACCA |
NGG |
2 |
0.56 |
Tier III |
| 7 |
NC_000077.6 |
11 |
102997363 |
+ |
Gm34620 |
NNNTCACCAGTACACTACCA |
NTG |
2 |
0.0308 |
Tier III |
Other clones with same target sequence:
(none)
