Construct: sgRNA BRDN0001148499

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CACTCACCAGTATACTACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCAKD (79877)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75873

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 45030102 + DCAKD NNNTCACCAGTATACTACCA NGG 0 1.0 Tier I
2 NC_000007.14 7 147688927 + CNTNAP2 NNNACACCAGAATACTACCA NGG 2 0.3571 Tier II
3 NC_000002.12 2 102724051 + MFSD9 NNNTCACCACTATACCACCA NGG 2 0.2667 Tier II
4 NC_000001.11 1 93127850 + MTF2 NNNTCACCACTATACTAACA NGG 2 0.2154 Tier II
5 NC_000012.12 12 122376384 - CLIP1 NNNTCACCACTATACTAACA NGG 2 0.2154 Tier II
6 NC_000013.11 13 36973117 - ALG5 NNNTCACCACTATACTAACA NGG 2 0.2154 Tier II
7 NC_000012.12 12 71477253 - LGR5 NNNTCACCAGTCTTCTACCA NGG 2 0.1404 Tier II
8 NC_000007.14 7 30319704 - ZNRF2 NNNTCACCAGTATCCTACTA NGG 2 0.0989 Tier II
9 NC_000008.11 8 22060809 - DMTN NNNTCACCAGGATACTACCC NGG 2 0.0909 Tier II
10 NC_000002.12 2 215405184 - FN1 NNNTCACCAGTATCCTACCA NGA 2 0.0149 Tier II
11 NC_000012.12 12 127651505 + LOC105370068 NNNTCCCCAGTATACTAACA NGG 2 0.2448 Tier III
12 NC_000010.11 10 101255929 + LINC02681 NNNTCACCACTATACTAACA NGG 2 0.2154 Tier III
13 NC_000005.10 5 16418477 - LINC02150 NNNTCACCAGCAGACTACCA NGG 2 0.1739 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 102997363 + Dcakd NNNTCACCAGTACACTACCA NTG 2 0.0308 Tier I
2 NC_000071.6 5 121366209 - Hectd4 NNNTCACAAGTATGCTACCA NGG 2 0.3033 Tier II
3 NC_000074.6 8 48610542 + Tenm3 NNNTCACCAGCATACTACCA NAG 2 0.1728 Tier II
4 NC_000076.6 10 84697485 + Polr3b NNNTCACCAGTATACTAGGA NGG 2 0.0167 Tier II
5 NC_000068.7 2 130981553 + Atrn NNNTCACCACTATACTACCA NTG 2 0.0156 Tier II
6 NC_000076.6 10 67404775 + Gm31962 NNNTCACCTATATACTACCA NGG 2 0.56 Tier III
7 NC_000077.6 11 102997363 + Gm34620 NNNTCACCAGTACACTACCA NTG 2 0.0308 Tier III
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Other clones with same target sequence:

(none)