Construct: sgRNA BRDN0001148500

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGAAGGTGTCTGGCAGAGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80260

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 75089645 + OR2AT4 NNNAAGGTCTCTGGCAGAGA NGG 2 0.2692 Tier I
2 NC_000011.10 11 64598527 + SLC22A12 NNNAAGGTGGCTGGCAGAGT NGG 2 0.15 Tier I
3 NC_000010.11 10 45787218 - WASHC2C NNNAAGGTGTCTGCCTGAGC NGG 2 0.0 Tier I
4 NC_000010.11 10 50127766 - WASHC2A NNNAAGGTGTCTGCCTGAGC NGG 2 0.0 Tier I
5 NC_000007.14 7 30781086 - MINDY4 NNNAAGGAGTCTGGCAGAGC NGG 1 0.8 Tier II
6 NC_000008.11 8 91339418 - SLC26A7 NNNAAGGTGAATGGCAGAGC NGG 2 0.6429 Tier II
7 NC_000010.11 10 116263472 + GFRA1 NNNAAGGTGAATGGCAGAGC NGG 2 0.6429 Tier II
8 NC_000012.12 12 119128697 + SRRM4 NNNGAAGTGTCTGGCAGAGC NGG 2 0.625 Tier II
9 NC_000011.10 11 124642993 - SIAE NNNAAGGTGTCAGACAGAGC NGG 2 0.6 Tier II
10 NC_000003.12 3 94088541 + NSUN3 NNNAAGGTAACTGGCAGAGC NGG 2 0.551 Tier II
11 NC_000005.10 5 64899322 + CWC27 NNNAAGGTGCCTGGCAGAGC NGG 1 0.5333 Tier II
12 NC_000010.11 10 103703920 - SH3PXD2A NNNAATCTGTCTGGCAGAGC NGG 2 0.4583 Tier II
13 NC_000005.10 5 10476247 - ROPN1L NNNGAGGGGTCTGGCAGAGC NGG 2 0.4583 Tier II
14 NC_000012.12 12 121782573 - RHOF NNNGAGGTGTCTGGCAGAGA NGG 2 0.3125 Tier II
15 NC_000009.12 9 117411067 + ASTN2 NNNTAGGTGTCTGGCAGACC NGG 2 0.2853 Tier II
16 NC_000017.11 17 82445580 - CYBC1 NNNCAGGGGTCTGGCAGAGC NGG 2 0.2588 Tier II
17 NC_000013.11 13 113227041 + CUL4A NNNAAGGTGTCTGGCAGTGA NGG 2 0.25 Tier II
18 NC_000003.12 3 202004 + CHL1 NNNAAAGTGTCTGGCACAGC NGG 2 0.2353 Tier II
19 NC_000006.12 6 33695900 - ITPR3 NNNAGGGTGTCTGGCAGAGT NGG 2 0.216 Tier II
20 NC_000008.11 8 58835822 + TOX NNNAAGGTGAGTGGCAGAGC NGG 2 0.2143 Tier II
21 NC_000005.10 5 122825383 - SNX2 NNNAAGGAGTCTGGCAGAGC NAG 2 0.2074 Tier II
22 NC_000017.11 17 6610385 - KIAA0753 NNNAAGGTGTCAGGCAGAGC NAG 2 0.2074 Tier II
23 NC_000008.11 8 143573229 - NAPRT NNNAAGGTGTCTGACAGAGC NAG 2 0.1944 Tier II
24 NC_000002.12 2 69745226 + ANXA4 NNNGAGGTGTTTGGCAGAGC NGG 2 0.1923 Tier II
25 NC_000017.11 17 76685936 + MXRA7 NNNCAGGTGTCGGGCAGAGC NGG 2 0.1765 Tier II
26 NC_000017.11 17 33382102 - ASIC2 NNNCCGGTGTCTGGCAGAGC NGG 2 0.1765 Tier II
27 NC_000007.14 7 134533653 + AKR1B10 NNNAATGTGTGTGGCAGAGC NGG 2 0.1667 Tier II
28 NC_000007.14 7 134572265 + AKR1B15 NNNAATGTGTGTGGCAGAGC NGG 2 0.1667 Tier II
29 NC_000017.11 17 72752020 + SLC39A11 NNNCAGGTGTCTCGCAGAGC NGG 2 0.1486 Tier II
30 NC_000007.14 7 15128163 - AGMO NNNAAGGTGTCTGCCAGAGT NGG 2 0.1286 Tier II
31 NC_000003.12 3 124153043 + KALRN NNNAAGGTTTCTGGCACAGC NGG 2 0.1255 Tier II
32 NC_000007.14 7 74056961 + ELN NNNAAGGTGTCTGGCAGGGT NGG 2 0.12 Tier II
33 NC_000001.11 1 53528397 - GLIS1 NNNCAGGTGTGTGGCAGAGC NGG 2 0.0882 Tier II
34 NC_000011.10 11 47344445 + MYBPC3 NNNCAGGTGTGTGGCAGAGC NGG 2 0.0882 Tier II
35 NC_000001.11 1 160681075 - CD48 NNNAAGGTGTCTGGGAGAGC NGG 1 0.05 Tier II
36 NC_000004.12 4 176754422 + VEGFC NNNAAGGTGTCTGGCAAAGC NTG 2 0.0364 Tier II
37 NC_000015.10 15 52292935 + MYO5C NNNAAGGAGTCTGGCAGAGC NTG 2 0.0312 Tier II
38 NC_000009.12 9 19856738 + SLC24A2 NNNAAGGTGTCTGGCAGTGC NTG 2 0.0195 Tier II
39 NC_000001.11 1 9328125 - SPSB1 NNNAAGGTGTGTGGCAGAGC NGA 2 0.0174 Tier II
40 NC_000002.12 2 290009 + LOC101927262 NNNAAAGTGTCCGGCAGAGC NGG 2 0.9474 Tier III
41 NC_000007.14 7 30781086 - INMT-MINDY4 NNNAAGGAGTCTGGCAGAGC NGG 1 0.8 Tier III
42 NC_000005.10 5 100247640 + LOC105379101 NNNAAGGTGTCAGGCAAAGC NGG 2 0.7467 Tier III
43 NC_000010.11 10 33002581 + ITGB1-DT NNNAAGAGGTCTGGCAGAGC NGG 2 0.7333 Tier III
44 NC_000006.12 6 29263001 + OR2U1P NNNAAGGTTTCTGACAGAGC NGG 2 0.4 Tier III
45 NC_000006.12 6 29263001 + LINC03003 NNNAAGGTTTCTGACAGAGC NGG 2 0.4 Tier III
46 NC_000001.11 1 89756218 - LOC107985743 NNNAAGTTGCCTGGCAGAGC NGG 2 0.3048 Tier III
47 NC_000005.10 5 122825383 - LOC105379154 NNNAAGGAGTCTGGCAGAGC NAG 2 0.2074 Tier III
48 NC_000001.11 1 219459826 + LYPLAL1-AS1 NNNATGGTGCCTGGCAGAGC NGG 2 0.1939 Tier III
49 NC_000008.11 8 93565897 - CIBAR1-DT NNNAAGGTTTCTTGCAGAGC NGG 2 0.16 Tier III
50 NC_000015.10 15 81329882 - TMC3-AS1 NNNCAGGTGTCTGCCAGAGC NGG 2 0.1513 Tier III
51 NC_000010.11 10 35697334 - RPL7P37 NNNAAGGTTTCTGGCACAGC NGG 2 0.1255 Tier III
52 NC_000010.11 10 110550819 + RPL7P35 NNNAAGGTTTCTGGCACAGC NGG 2 0.1255 Tier III
53 NC_000013.11 13 40439909 - LINC00598 NNNAAGGTGTCTTGGAGAGC NGG 2 0.015 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 43049783 + Tgfbrap1 NNNAAGGGTTCTGGCAGAGC NGG 2 0.3911 Tier I
2 NC_000083.6 17 34449253 - BC051142 NNNAAGGTGTCTGGCGGAGC NGA 2 0.0134 Tier I
3 NC_000077.6 11 93581497 - Car10 NNNAACGTGTCTGGCAGAGC NGG 1 0.6818 Tier II
4 NC_000078.6 12 78871501 + Eif2s1 NNNAGGGTGACTGGCAGAGC NGG 2 0.6171 Tier II
5 NC_000077.6 11 100118676 - Krt13 NNNACAGTGTCTGGCAGAGC NGG 2 0.5 Tier II
6 NC_000073.6 7 127244122 + Zfp771 NNNAAGCTGTCTGGCAGATC NGG 2 0.4583 Tier II
7 NC_000078.6 12 105479134 + D430019H16Rik NNNGAGGGGTCTGGCAGAGC NGG 2 0.4583 Tier II
8 NC_000071.6 5 127280486 - Tmem132c NNNAAGGTGTCAGGCAGTGC NGG 2 0.4 Tier II
9 NC_000081.6 15 36193714 - Spag1 NNNAAGGAGTCTGGCAGGGC NGG 2 0.32 Tier II
10 NC_000074.6 8 106260051 - Smpd3 NNNCAGGAGTCTGGCAGAGC NGG 2 0.2824 Tier II
11 NC_000076.6 10 76825967 + Pcbp3 NNNAAGGTCTCTGGCAGGGC NGG 2 0.2154 Tier II
12 NC_000085.6 19 38006340 + Myof NNNAAGGTGGTTGGCAGAGC NGG 2 0.1538 Tier II
13 NC_000074.6 8 9412389 + Fam155a NNNAAGCTGTCTGGAAGAGC NGG 2 0.1528 Tier II
14 NC_000082.6 16 31992090 - Senp5 NNNAAGGTGTCTGGCAGACT NGG 2 0.1345 Tier II
15 NC_000074.6 8 115206134 - Wwox NNNAAGGTGGCTGGCAGAGC NAG 2 0.1296 Tier II
16 NC_000074.6 8 114809056 + Wwox NNNAAGGTGTCTGGCAGTGC NAG 2 0.1296 Tier II
17 NC_000069.6 3 96026480 - Vps45 NNNAAGGTGCCTGGCACAGC NGG 2 0.1255 Tier II
18 NC_000076.6 10 128715416 - Pmel NNNAAGGTGTCTGGAAGGGC NGG 2 0.0889 Tier II
19 NC_000070.6 4 135336630 + Srrm1 NNNAAGGTGTGTGTCAGAGC NGG 2 0.0667 Tier II
20 NC_000067.6 1 73015675 + Tnp1 NNNAAGGTGTCTGGAAGAGC NAG 2 0.0576 Tier II
21 NC_000071.6 5 127627097 + Slc15a4 NNNAAGGTGTATGGCAGAGG NGG 2 0.0441 Tier II
22 NC_000073.6 7 97436481 - Kctd14 NNNAAGGTGACTGGGAGAGC NGG 2 0.0429 Tier II
23 NC_000069.6 3 110132482 + Ntng1 NNNAGGGTGTCTGGCAGAGG NGG 2 0.0424 Tier II
24 NC_000074.6 8 79057316 + Zfp827 NNNAAGGTGTCTGGCAGAGC NGC 1 0.0222 Tier II
25 NC_000075.6 9 37638994 + Siae NNNATGGTGTCTGGCAGAGC NTG 2 0.0142 Tier II
26 NC_000076.6 10 89432216 + Gas2l3 NNNGAGGTGTCTGGCAGAGC NGC 2 0.0139 Tier II
27 NC_000071.6 5 92931599 - Shroom3 NNNAAGGTGTCTGTCAGAGC NTG 2 0.0104 Tier II
28 NC_000079.6 13 59453206 - Agtpbp1 NNNAAGGTGTCTGGGAGAGC NGA 2 0.0035 Tier II
29 NC_000067.6 1 188937321 + Ush2a NNNAAGGTGTCTGGCAGAGG NGC 2 0.0013 Tier II
30 NC_000076.6 10 60080349 + Ascc1 NNNAAGGTGGCTGGCTGAGC NGG 2 0.0 Tier II
31 NC_000078.6 12 37294251 + Agmo NNNAAGGTTTCTGGCTGAGC NGG 2 0.0 Tier II
32 NC_000072.6 6 25130947 + Gm30658 NNNAACATGTCTGGCAGAGC NGG 2 0.6818 Tier III
33 NC_000083.6 17 14517740 + Gm34916 NNNACAGTGTCTGGCAGAGC NGG 2 0.5 Tier III
34 NC_000071.6 5 127280486 - Tmem132cos NNNAAGGTGTCAGGCAGTGC NGG 2 0.4 Tier III
35 NC_000076.6 10 60228439 + Gm2038 NNNAAGGTGTCTGGAGGAGC NGG 2 0.0427 Tier III
36 NC_000078.6 12 32936631 + 4933406C10Rik NNNAAGGTGTCTGGCTGACC NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)