Construct: sgRNA BRDN0001148502

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTTTACCTGATAGTCCACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A2 (1459)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75588

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 58167695 + CSNK2A2 NNNTTACCTGATAGTCCACG NGG 0 1.0 Tier I
2 NC_000015.10 15 25684076 + ATP10A NNNTGACCTGATTGTCCACG NGG 2 0.192 Tier II
3 NC_000002.12 2 47987708 + LOC105374591 NNNATACCTGATAGTCCACT NGG 2 0.3333 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 95460688 + Csnk2a2 NNNTCACCTGATAGTCCACA NGG 2 0.9375 Tier I
2 NC_000068.7 2 37500531 + Rabgap1 NNNTTACCTGATAGTCCATG NGG 1 0.4615 Tier II
3 NC_000070.6 4 47038132 - Anks6 NNNTTACCTCATAGTCCAAG NGG 2 0.1714 Tier II
4 NC_000083.6 17 56361289 + Kdm4b NNNTTACCTGATAGTGCAGG NGG 2 0.0192 Tier II
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Other clones with same target sequence:

(none)