Construct: sgRNA BRDN0001148504

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCGTCTGGGATATGACACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKM (5213)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77075

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 48135319 + PFKM NNNTCTGGGATATGACACCC NGG 0 1.0 Tier I
2 NC_000007.14 7 5973418 + PMS2 NNNTCTGAGAAATGACACCC NGG 2 0.75 Tier I
3 NC_000011.10 11 120240567 + POU2F3 NNNTCTGGGATAAGAAACCC NGG 2 0.6923 Tier II
4 NC_000010.11 10 126498902 - C10orf90 NNNTCTGGGATATGACATCC NGG 1 0.6429 Tier II
5 NC_000001.11 1 42154181 - GUCA2B NNNTCAGGGATGTGACACCC NGG 2 0.6259 Tier II
6 NC_000016.10 16 14226636 + MRTFB NNNTCTTGGATATGACACCA NGG 2 0.2857 Tier II
7 NC_000011.10 11 63538989 - PLAAT4 NNNTCTTGGAGATGACACCC NGG 2 0.2286 Tier II
8 NC_000015.10 15 73464522 - REC114 NNNTCTTGGAGATGACACCC NGG 2 0.2286 Tier II
9 NC_000003.12 3 73461889 - PDZRN3 NNNTCTGGGAGATGACAACC NGG 2 0.2154 Tier II
10 NC_000011.10 11 8888538 + DENND2B NNNTCTGGGATATGACACAA NGG 2 0.2143 Tier II
11 NC_000005.10 5 159192762 + RNF145 NNNTCTGGGAAATGACACCC NAG 2 0.1944 Tier II
12 NC_000015.10 15 92161362 + SLCO3A1 NNNTCTGGGATGGGACACCC NGG 2 0.1884 Tier II
13 NC_000003.12 3 60854137 - FHIT NNNTGTGGGATATGATACCC NGG 2 0.1846 Tier II
14 NC_000011.10 11 96255579 + MAML2 NNNTCTGGCCTATGACACCC NGG 2 0.1795 Tier II
15 NC_000005.10 5 142891990 - ARHGAP26 NNNTCTGGGATCTGGCACCC NGG 2 0.1711 Tier II
16 NC_000011.10 11 2426031 + TRPM5 NNNTCTGGGATAGGACATCC NGG 2 0.1677 Tier II
17 NC_000022.11 22 39579099 + CACNA1I NNNACTGGGCTATGACACCC NGG 2 0.1587 Tier II
18 NC_000012.12 12 32544942 - FGD4 NNNTCTGGGATATGAGACCC NGG 1 0.1538 Tier II
19 NC_000015.10 15 58605668 - ADAM10 NNNTCTGGGATATAAGACCC NGG 2 0.1154 Tier II
20 NC_000023.11 X 45186792 - DIPK2B NNNTCTGGGAGATGACACCC NAG 2 0.1037 Tier II
21 NC_000005.10 5 33840731 + ADAMTS12 NNNTCTGGCATATGAGACCC NGG 2 0.0828 Tier II
22 NC_000020.11 20 32577390 + NOL4L NNNTCTGGGATCTGACACCT NGG 2 0.0789 Tier II
23 NC_000011.10 11 19881438 - NAV2 NNNTCTGGGAAATGACACCC NGA 2 0.0521 Tier II
24 NC_000002.12 2 66521546 + MEIS1 NNNTCTGGGGTATGACACCG NGG 2 0.0327 Tier II
25 NC_000005.10 5 94967522 - MCTP1 NNNTCAGGGATATGACACCC NGC 2 0.0193 Tier II
26 NC_000007.14 7 6751374 - PMS2CL NNNTCTGAGAAATGACACCC NGG 2 0.75 Tier III
27 NC_000010.11 10 126498902 - LOC105378549 NNNTCTGGGATATGACATCC NGG 1 0.6429 Tier III
28 NC_000016.10 16 81159156 + PKD1L2 NNNTCTTGGAAATGACACCC NGG 2 0.4286 Tier III
29 NC_000002.12 2 236736890 - LOC105373948 NNNTCTTGGATATAACACCC NGG 2 0.4286 Tier III
30 NC_000017.11 17 27231775 - LOC105371704 NNNTCTGGGACATTACACCC NGG 2 0.1778 Tier III
31 NC_000022.11 22 39155821 - FUNDC2P4 NNNTCTGGGATATGAAACCC NTG 2 0.039 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 98123174 + Pfkm NNNCCTTGGATATGACACCC NGG 2 0.3697 Tier I
2 NC_000079.6 13 6605701 - Pfkp NNNTTTGGGATTTGACACCC NGG 2 0.2121 Tier I
3 NC_000067.6 1 74621482 + Stk36 NNNTCTGGGATAAGACACCC NGG 1 0.6923 Tier II
4 NC_000079.6 13 92235450 + Msh3 NNNTCAGGGAAATGACACCC NGG 2 0.65 Tier II
5 NC_000080.6 14 69809956 - Rhobtb2 NNNTCGAGGATATGACACCC NGG 2 0.5714 Tier II
6 NC_000067.6 1 43522531 - Nck2 NNNTCTGGGGTATGACACCC NGG 1 0.5556 Tier II
7 NC_000074.6 8 72007602 - Cyp4f18 NNNTCTGGGATAAGGCACCC NGG 2 0.45 Tier II
8 NC_000068.7 2 76140530 - Pde11a NNNTCGGGGATGTGACACCC NGG 2 0.4127 Tier II
9 NC_000070.6 4 119389046 + Ccdc30 NNNTCTTGGATATGACACCA NGG 2 0.2857 Tier II
10 NC_000084.6 18 67958436 + Ldlrad4 NNNTCTGGGATATCACACCA NGG 2 0.2143 Tier II
11 NC_000086.7 X 151620138 + Phf8 NNNTCTGGGTTATGTCACCC NGG 2 0.1765 Tier II
12 NC_000071.6 5 135024212 - Stx1a NNNTCTGGGAAATGACAGCC NGG 2 0.1 Tier II
13 NC_000080.6 14 64973626 - Ints9 NNNTCTGGGATCTGACACCC NCG 2 0.0282 Tier II
14 NC_000068.7 2 156708774 - Dlgap4 NNNTCTGGGATATGAGACCC NGT 2 0.0025 Tier II
15 NC_000074.6 8 72007602 - Gm51535 NNNTCTGGGATAAGGCACCC NGG 2 0.45 Tier III
16 NC_000076.6 10 117559529 + Gm32235 NNNTCTGGGCTATGTCACCC NGG 2 0.0667 Tier III
17 NC_000080.6 14 99678811 - Gm34061 NNNTCTGGGATCTGACAGCC NGG 2 0.0351 Tier III
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Other clones with same target sequence:

(none)