Construct: sgRNA BRDN0001148505
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGGCGTCATAACTTAGAGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAOK1 (57551)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76806
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
29517637 |
+ |
TAOK1 |
NNNGCGTCATAACTTAGAGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
39563511 |
+ |
PABPC4 |
NNNGCGTCATAACACAGAGC |
NGG |
2 |
0.1688 |
Tier II |
| 3 |
NC_000005.10 |
5 |
59876553 |
- |
PDE4D |
NNNGAGTCATAACTTAGAGC |
NGT |
2 |
0.0092 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
54945105 |
+ |
Tnip1 |
NNNGCCTCATGACTTAGAGC |
NGG |
2 |
0.4432 |
Tier II |
| 2 |
NC_000079.6 |
13 |
15562330 |
+ |
Gli3 |
NNNGCTTCATCACTTAGAGC |
NGG |
2 |
0.2667 |
Tier II |
| 3 |
NC_000075.6 |
9 |
50768181 |
+ |
1110032A03Rik |
NNNGCGTCATCACTTAGTGC |
NGG |
2 |
0.2 |
Tier II |
Other clones with same target sequence:
(none)
