Construct: sgRNA BRDN0001148507
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGCTGGATGTGATACATG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PRKCB (5579)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000016.10 | 16 | 24172317 | - | PRKCB | NNNGCTGGATGTGATACATG | NGG | 0 | 1.0 | Tier I |
2 | NC_000017.11 | 17 | 66738811 | - | PRKCA | NNNGCTGAATGTGGTACATG | NGG | 2 | 0.4667 | Tier I |
3 | NC_000001.11 | 1 | 19139913 | + | UBR4 | NNNGCTGGATGAGATACATG | NGG | 1 | 0.8 | Tier II |
4 | NC_000009.12 | 9 | 5749085 | + | RIC1 | NNNACTGGATTTGATACATG | NGG | 2 | 0.3462 | Tier II |
5 | NC_000007.14 | 7 | 83441712 | - | SEMA3E | NNNGCTGGATGTGTAACATG | NGG | 2 | 0.3088 | Tier II |
6 | NC_000001.11 | 1 | 147632458 | + | ACP6 | NNNGCTGGTTGTGGTACATG | NGG | 2 | 0.28 | Tier II |
7 | NC_000003.12 | 3 | 169106258 | - | MECOM | NNNGCTGAATGTGATACATG | NAG | 2 | 0.2593 | Tier II |
8 | NC_000014.9 | 14 | 93509646 | - | UNC79 | NNNCCTGGATGTGATACATG | NAG | 2 | 0.1373 | Tier II |
9 | NC_000023.11 | X | 105084439 | + | IL1RAPL2 | NNNCCTGGATGTGATACATG | NAG | 2 | 0.1373 | Tier II |
10 | NC_000022.11 | 22 | 29490958 | + | NEFH | NNNGCTGAATGTGATACATG | NCG | 2 | 0.1071 | Tier II |
11 | NC_000005.10 | 5 | 184992 | + | PLEKHG4B | NNNGCTGGATGTGATACGTG | NAG | 2 | 0.1037 | Tier II |
12 | NC_000001.11 | 1 | 150661709 | - | GOLPH3L | NNNGCTGGATGAGATAGATG | NGG | 2 | 0.0471 | Tier II |
13 | NC_000009.12 | 9 | 33399916 | + | AQP7 | NNNACTGGATGTGAGACATG | NGG | 2 | 0.045 | Tier II |
14 | NC_000002.12 | 2 | 143476858 | + | ARHGAP15 | NNNGCTGGATGTGTTACATG | NGA | 2 | 0.037 | Tier II |
15 | NC_000019.10 | 19 | 16531827 | + | CHERP | NNNGCTGCATGTGATAGATG | NGG | 2 | 0.0362 | Tier II |
16 | NC_000003.12 | 3 | 134569521 | - | CEP63 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
17 | NC_000004.12 | 4 | 133988524 | - | PABPC4L | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
18 | NC_000006.12 | 6 | 45271909 | + | SUPT3H | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
19 | NC_000007.14 | 7 | 89205608 | - | ZNF804B | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
20 | NC_000011.10 | 11 | 114598219 | - | NXPE4 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
21 | NC_000011.10 | 11 | 114598219 | - | NXPE2 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
22 | NC_000013.11 | 13 | 52097477 | + | NEK5 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
23 | NC_000015.10 | 15 | 38291223 | - | SPRED1 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
24 | NC_000015.10 | 15 | 86831584 | - | AGBL1 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
25 | NC_000023.11 | X | 74866227 | + | NEXMIF | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier II |
26 | NC_000019.10 | 19 | 52486132 | + | ZNF578 | NNNGCTGGAGGTGAGACATG | NGG | 2 | 0.025 | Tier II |
27 | NC_000010.11 | 10 | 119434565 | - | GRK5 | NNNTCTGGATGTGAGACATG | NGG | 2 | 0.0182 | Tier II |
28 | NC_000015.10 | 15 | 84030392 | + | ADAMTSL3 | NNNTCTGGATGTGAGACATG | NGG | 2 | 0.0182 | Tier II |
29 | NC_000007.14 | 7 | 123795060 | + | HYAL4 | NNNGCTGGATGTGAGACATG | NAG | 2 | 0.013 | Tier II |
30 | NC_000012.12 | 12 | 28399798 | - | CCDC91 | NNNGCTGGATGTGAGACATG | NAG | 2 | 0.013 | Tier II |
31 | NC_000021.9 | 21 | 41334947 | + | FAM3B | NNNGCTGGATGTGAGACATG | NAG | 2 | 0.013 | Tier II |
32 | NC_000001.11 | 1 | 203067637 | + | PPFIA4 | NNNGCTGGATGTGAGACAGG | NGG | 2 | 0.0125 | Tier II |
33 | NC_000019.10 | 19 | 51869304 | + | ZNF577 | NNNGCTGGATGTGAGACATG | NTG | 2 | 0.0019 | Tier II |
34 | NC_000020.11 | 20 | 1152008 | + | PSMF1 | NNNGCTGGATTTGATCCATG | NGG | 2 | 0.0 | Tier II |
35 | NC_000023.11 | X | 141634014 | + | SPANXA2-OT1 | NNNACTGGATGTGATACATG | NAG | 2 | 0.2333 | Tier III |
36 | NC_000011.10 | 11 | 90745388 | + | DISC1FP1 | NNNCCTGGATGTGATACATG | NAG | 2 | 0.1373 | Tier III |
37 | NC_000011.10 | 11 | 127320519 | - | LINC02712 | NNNCCTGGATGTGATACATG | NAG | 2 | 0.1373 | Tier III |
38 | NC_000014.9 | 14 | 49236702 | + | LOC105378178 | NNNCCTGGATGTGATACATG | NAG | 2 | 0.1373 | Tier III |
39 | NC_000009.12 | 9 | 11424658 | - | LOC105375974 | NNNGCTGGATGTGACACATG | NAG | 2 | 0.0707 | Tier III |
40 | NC_000002.12 | 2 | 13177121 | - | LOC105373436 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
41 | NC_000002.12 | 2 | 13177121 | - | LOC105373484 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
42 | NC_000002.12 | 2 | 13206388 | + | LOC105373436 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
43 | NC_000002.12 | 2 | 13206388 | + | LOC105373484 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
44 | NC_000004.12 | 4 | 106774088 | + | LOC105377356 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
45 | NC_000009.12 | 9 | 81955525 | - | LOC105376105 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
46 | NC_000011.10 | 11 | 38242721 | - | LOC105376634 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
47 | NC_000013.11 | 13 | 84156332 | + | LINC00333 | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
48 | NC_000014.9 | 14 | 106551527 | + | IGH | NNNCCTGGATGTGAGACATG | NGG | 2 | 0.0265 | Tier III |
49 | NC_000010.11 | 10 | 107973723 | + | LINC01435 | NNNGCTGGATGTGAGACATG | NAG | 2 | 0.013 | Tier III |
50 | NC_000001.11 | 1 | 203067637 | + | LOC105371687 | NNNGCTGGATGTGAGACAGG | NGG | 2 | 0.0125 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000077.6 | 11 | 107981656 | + | Prkca | NNNGCTGAATGTGGTACATG | NGG | 2 | 0.4667 | Tier I |
2 | NC_000073.6 | 7 | 122582477 | - | Prkcb | NNNGTTGGATGTGGTACATG | NGG | 2 | 0.297 | Tier I |
3 | NC_000069.6 | 3 | 141589225 | + | Unc5c | NNNGGTGGATGTGATACATA | NGG | 2 | 0.5625 | Tier II |
4 | NC_000083.6 | 17 | 10456171 | + | Pacrg | NNNGCAGCATGTGATACATG | NGG | 2 | 0.5333 | Tier II |
5 | NC_000067.6 | 1 | 134146997 | - | Chit1 | NNNGATGGATGTGAAACATG | NGG | 2 | 0.3308 | Tier II |
6 | NC_000067.6 | 1 | 20133812 | + | Pkhd1 | NNNGATGGCTGTGATACATG | NGG | 2 | 0.3265 | Tier II |
7 | NC_000077.6 | 11 | 32397961 | - | Sh3pxd2b | NNNCCTGGATGTCATACATG | NGG | 2 | 0.2229 | Tier II |
8 | NC_000077.6 | 11 | 69790988 | + | Chrnb1 | NNNGCTGGATGTCATAAATG | NGG | 2 | 0.1965 | Tier II |
9 | NC_000081.6 | 15 | 86430606 | + | Tbc1d22a | NNNGCTGGATGTGTCACATG | NGG | 2 | 0.1455 | Tier II |
10 | NC_000073.6 | 7 | 140109211 | + | Echs1 | NNNGCTGGATGTGGTACATG | NGA | 2 | 0.0324 | Tier II |
11 | NC_000076.6 | 10 | 108412720 | + | Pawr | NNNGCTGGATGTTATAGATG | NGG | 2 | 0.0176 | Tier II |
12 | NC_000079.6 | 13 | 45809581 | - | Atxn1 | NNNGCTGGATGTGACAGATG | NGG | 2 | 0.016 | Tier II |