Construct: sgRNA BRDN0001148507

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTGCTGGATGTGATACATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCB (5579)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75954

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 24172317 - PRKCB NNNGCTGGATGTGATACATG NGG 0 1.0 Tier I
2 NC_000017.11 17 66738811 - PRKCA NNNGCTGAATGTGGTACATG NGG 2 0.4667 Tier I
3 NC_000001.11 1 19139913 + UBR4 NNNGCTGGATGAGATACATG NGG 1 0.8 Tier II
4 NC_000009.12 9 5749085 + RIC1 NNNACTGGATTTGATACATG NGG 2 0.3462 Tier II
5 NC_000007.14 7 83441712 - SEMA3E NNNGCTGGATGTGTAACATG NGG 2 0.3088 Tier II
6 NC_000001.11 1 147632458 + ACP6 NNNGCTGGTTGTGGTACATG NGG 2 0.28 Tier II
7 NC_000003.12 3 169106258 - MECOM NNNGCTGAATGTGATACATG NAG 2 0.2593 Tier II
8 NC_000014.9 14 93509646 - UNC79 NNNCCTGGATGTGATACATG NAG 2 0.1373 Tier II
9 NC_000023.11 X 105084439 + IL1RAPL2 NNNCCTGGATGTGATACATG NAG 2 0.1373 Tier II
10 NC_000022.11 22 29490958 + NEFH NNNGCTGAATGTGATACATG NCG 2 0.1071 Tier II
11 NC_000005.10 5 184992 + PLEKHG4B NNNGCTGGATGTGATACGTG NAG 2 0.1037 Tier II
12 NC_000001.11 1 150661709 - GOLPH3L NNNGCTGGATGAGATAGATG NGG 2 0.0471 Tier II
13 NC_000009.12 9 33399916 + AQP7 NNNACTGGATGTGAGACATG NGG 2 0.045 Tier II
14 NC_000002.12 2 143476858 + ARHGAP15 NNNGCTGGATGTGTTACATG NGA 2 0.037 Tier II
15 NC_000019.10 19 16531827 + CHERP NNNGCTGCATGTGATAGATG NGG 2 0.0362 Tier II
16 NC_000003.12 3 134569521 - CEP63 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
17 NC_000004.12 4 133988524 - PABPC4L NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
18 NC_000006.12 6 45271909 + SUPT3H NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
19 NC_000007.14 7 89205608 - ZNF804B NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
20 NC_000011.10 11 114598219 - NXPE4 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
21 NC_000011.10 11 114598219 - NXPE2 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
22 NC_000013.11 13 52097477 + NEK5 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
23 NC_000015.10 15 38291223 - SPRED1 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
24 NC_000015.10 15 86831584 - AGBL1 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
25 NC_000023.11 X 74866227 + NEXMIF NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier II
26 NC_000019.10 19 52486132 + ZNF578 NNNGCTGGAGGTGAGACATG NGG 2 0.025 Tier II
27 NC_000010.11 10 119434565 - GRK5 NNNTCTGGATGTGAGACATG NGG 2 0.0182 Tier II
28 NC_000015.10 15 84030392 + ADAMTSL3 NNNTCTGGATGTGAGACATG NGG 2 0.0182 Tier II
29 NC_000007.14 7 123795060 + HYAL4 NNNGCTGGATGTGAGACATG NAG 2 0.013 Tier II
30 NC_000012.12 12 28399798 - CCDC91 NNNGCTGGATGTGAGACATG NAG 2 0.013 Tier II
31 NC_000021.9 21 41334947 + FAM3B NNNGCTGGATGTGAGACATG NAG 2 0.013 Tier II
32 NC_000001.11 1 203067637 + PPFIA4 NNNGCTGGATGTGAGACAGG NGG 2 0.0125 Tier II
33 NC_000019.10 19 51869304 + ZNF577 NNNGCTGGATGTGAGACATG NTG 2 0.0019 Tier II
34 NC_000020.11 20 1152008 + PSMF1 NNNGCTGGATTTGATCCATG NGG 2 0.0 Tier II
35 NC_000023.11 X 141634014 + SPANXA2-OT1 NNNACTGGATGTGATACATG NAG 2 0.2333 Tier III
36 NC_000011.10 11 90745388 + DISC1FP1 NNNCCTGGATGTGATACATG NAG 2 0.1373 Tier III
37 NC_000011.10 11 127320519 - LINC02712 NNNCCTGGATGTGATACATG NAG 2 0.1373 Tier III
38 NC_000014.9 14 49236702 + LOC105378178 NNNCCTGGATGTGATACATG NAG 2 0.1373 Tier III
39 NC_000009.12 9 11424658 - LOC105375974 NNNGCTGGATGTGACACATG NAG 2 0.0707 Tier III
40 NC_000002.12 2 13177121 - LOC105373436 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
41 NC_000002.12 2 13177121 - LOC105373484 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
42 NC_000002.12 2 13206388 + LOC105373436 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
43 NC_000002.12 2 13206388 + LOC105373484 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
44 NC_000004.12 4 106774088 + LOC105377356 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
45 NC_000009.12 9 81955525 - LOC105376105 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
46 NC_000011.10 11 38242721 - LOC105376634 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
47 NC_000013.11 13 84156332 + LINC00333 NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
48 NC_000014.9 14 106551527 + IGH NNNCCTGGATGTGAGACATG NGG 2 0.0265 Tier III
49 NC_000010.11 10 107973723 + LINC01435 NNNGCTGGATGTGAGACATG NAG 2 0.013 Tier III
50 NC_000001.11 1 203067637 + LOC105371687 NNNGCTGGATGTGAGACAGG NGG 2 0.0125 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 107981656 + Prkca NNNGCTGAATGTGGTACATG NGG 2 0.4667 Tier I
2 NC_000073.6 7 122582477 - Prkcb NNNGTTGGATGTGGTACATG NGG 2 0.297 Tier I
3 NC_000069.6 3 141589225 + Unc5c NNNGGTGGATGTGATACATA NGG 2 0.5625 Tier II
4 NC_000083.6 17 10456171 + Pacrg NNNGCAGCATGTGATACATG NGG 2 0.5333 Tier II
5 NC_000067.6 1 134146997 - Chit1 NNNGATGGATGTGAAACATG NGG 2 0.3308 Tier II
6 NC_000067.6 1 20133812 + Pkhd1 NNNGATGGCTGTGATACATG NGG 2 0.3265 Tier II
7 NC_000077.6 11 32397961 - Sh3pxd2b NNNCCTGGATGTCATACATG NGG 2 0.2229 Tier II
8 NC_000077.6 11 69790988 + Chrnb1 NNNGCTGGATGTCATAAATG NGG 2 0.1965 Tier II
9 NC_000081.6 15 86430606 + Tbc1d22a NNNGCTGGATGTGTCACATG NGG 2 0.1455 Tier II
10 NC_000073.6 7 140109211 + Echs1 NNNGCTGGATGTGGTACATG NGA 2 0.0324 Tier II
11 NC_000076.6 10 108412720 + Pawr NNNGCTGGATGTTATAGATG NGG 2 0.0176 Tier II
12 NC_000079.6 13 45809581 - Atxn1 NNNGCTGGATGTGACAGATG NGG 2 0.016 Tier II
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Other clones with same target sequence:

(none)