Construct: sgRNA BRDN0001148512
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGATCTCCTCCACTCGCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP4 (58538)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77113
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
201692932 |
- |
MPP4 |
NNNATCTCCTCCACTCGCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
52058714 |
- |
NR4A1 |
NNNAGCTCCTCCACCCGCCG |
NGG |
2 |
0.1745 |
Tier I |
| 3 |
NC_000011.10 |
11 |
113773580 |
+ |
ZW10 |
NNNATCTCCTCCACCCGCCG |
NGT |
2 |
0.0044 |
Tier I |
| 4 |
NC_000010.11 |
10 |
127195425 |
- |
DOCK1 |
NNNGTCTCCTCCACTCGCTG |
NGG |
2 |
0.2885 |
Tier II |
| 5 |
NC_000010.11 |
10 |
127195425 |
- |
INSYN2A |
NNNGTCTCCTCCACTCGCTG |
NGG |
2 |
0.2885 |
Tier II |
| 6 |
NC_000020.11 |
20 |
47183483 |
+ |
EYA2 |
NNNATTTCCTCCACTCCCCG |
NGG |
2 |
0.2185 |
Tier II |
| 7 |
NC_000002.12 |
2 |
47527591 |
+ |
MSH2 |
NNNATCTCCTCCACTCTCTG |
NGG |
2 |
0.1154 |
Tier II |
| 8 |
NC_000002.12 |
2 |
47527591 |
+ |
KCNK12 |
NNNATCTCCTCCACTCTCTG |
NGG |
2 |
0.1154 |
Tier II |
| 9 |
NC_000019.10 |
19 |
4581748 |
+ |
LOC105372249 |
NNNATCTCCTCCGCCCGCCG |
NGG |
2 |
0.1779 |
Tier III |
| 10 |
NC_000011.10 |
11 |
134958565 |
+ |
LINC02697 |
NNNATCTCCTCCACCCGCAG |
NGG |
2 |
0.1169 |
Tier III |
| 11 |
NC_000002.12 |
2 |
47527591 |
+ |
MSH2-OT1 |
NNNATCTCCTCCACTCTCTG |
NGG |
2 |
0.1154 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
49055770 |
- |
Zw10 |
NNNATCTCCTCCACCCGCCG |
NGT |
2 |
0.0044 |
Tier I |
| 2 |
NC_000083.6 |
17 |
79590727 |
+ |
Rmdn2 |
NNNATCACCTCCACTCGCCT |
NGG |
2 |
0.6125 |
Tier II |
| 3 |
NC_000082.6 |
16 |
15589644 |
- |
Ube2v2 |
NNNATCTCCTCCACTCTCCT |
NGG |
2 |
0.175 |
Tier II |
| 4 |
NC_000080.6 |
14 |
32680908 |
+ |
3425401B19Rik |
NNNATCTCCTCCACTGGCCT |
NGG |
2 |
0.1077 |
Tier II |
| 5 |
NC_000073.6 |
7 |
100211599 |
- |
Gm34877 |
NNNATCTCCTCCACTCTCCG |
NTG |
2 |
0.0097 |
Tier III |
Other clones with same target sequence:
(none)
