Construct: sgRNA BRDN0001148514
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGTCCAACAACGATCCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNK2 (10188)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75583
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
195882308 |
+ |
TNK2 |
NNNTCCAACAACGATCCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
76120388 |
+ |
PCSK5 |
NNNTCCAAAAACAATCCCAG |
NGG |
2 |
0.7912 |
Tier II |
| 3 |
NC_000001.11 |
1 |
33117218 |
- |
AZIN2 |
NNNTCCAACAACAATCACAG |
NGG |
2 |
0.4308 |
Tier II |
| 4 |
NC_000010.11 |
10 |
75745739 |
+ |
LRMDA |
NNNTCCAGCAACGAACCCAG |
NGG |
2 |
0.4246 |
Tier II |
| 5 |
NC_000006.12 |
6 |
105994268 |
- |
PRDM1 |
NNNTCCAACAACGACCCCGG |
NGG |
2 |
0.1023 |
Tier II |
| 6 |
NC_000003.12 |
3 |
188209273 |
- |
LPP |
NNNTCCAACAACTATCCCAG |
NAG |
2 |
0.0778 |
Tier II |
| 7 |
NC_000017.11 |
17 |
75667777 |
- |
SAP30BP |
NNNTCAAACAACGAGCCCAG |
NGG |
2 |
0.0464 |
Tier II |
| 8 |
NC_000011.10 |
11 |
61045349 |
- |
LOC105369325 |
NNNTCCAATAAAGATCCCAG |
NGG |
2 |
0.625 |
Tier III |
| 9 |
NC_000013.11 |
13 |
78975412 |
- |
LOC105370273 |
NNNTACAACAAAGATCCCAG |
NGG |
2 |
0.4082 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
32669974 |
- |
Tnk2 |
NNNTCCAACAAAGATCCCAG |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000074.6 |
8 |
119426227 |
+ |
Osgin1 |
NNNTCCCACAATGATCCCAG |
NGG |
2 |
0.2356 |
Tier II |
| 3 |
NC_000076.6 |
10 |
89669144 |
+ |
Scyl2 |
NNNTCCAACAGCGATCCCAG |
NAG |
2 |
0.1685 |
Tier II |
| 4 |
NC_000067.6 |
1 |
123990391 |
- |
Dpp10 |
NNNTCCAACAACGCTCCCAC |
NGG |
2 |
0.0918 |
Tier II |
| 5 |
NC_000083.6 |
17 |
36188854 |
- |
H2-T3 |
NNNTCCAACATCGACCCCAG |
NGG |
2 |
0.0839 |
Tier II |
| 6 |
NC_000075.6 |
9 |
70260574 |
- |
Myo1e |
NNNTCCAACAAGGATCCCAG |
NGC |
2 |
0.0099 |
Tier II |
| 7 |
NC_000074.6 |
8 |
119426227 |
+ |
Gm32006 |
NNNTCCCACAATGATCCCAG |
NGG |
2 |
0.2356 |
Tier III |
Other clones with same target sequence:
(none)
