Construct: sgRNA BRDN0001148522
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGTAGATATCCTGATCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CD2 (914)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75828
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
116754871 |
- |
CD2 |
NNNGTAGATATCCTGATCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
97234575 |
- |
ARHGAP19 |
NNNGTAGATAACTTGATCAT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000014.9 |
14 |
76449163 |
- |
ESRRB |
NNNGTGGATATCCTGATCAT |
NAG |
2 |
0.1852 |
Tier II |
| 4 |
NC_000014.9 |
14 |
75150401 |
- |
TMED10 |
NNNGAAGATATCCTGATCAT |
NTG |
2 |
0.0195 |
Tier II |
| 5 |
NC_000010.11 |
10 |
97234575 |
- |
ARHGAP19-SLIT1 |
NNNGTAGATAACTTGATCAT |
NGG |
2 |
0.525 |
Tier III |
| 6 |
NC_000002.12 |
2 |
2719209 |
+ |
LOC105373390 |
NNNGTAGACATCATGATCAT |
NGG |
2 |
0.355 |
Tier III |
| 7 |
NC_000005.10 |
5 |
117568067 |
+ |
LINC00992 |
NNNGTAGATATGCTGATCAC |
NGG |
2 |
0.0404 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
34583256 |
+ |
Amer3 |
NNNGTAGAGATCTTGATCAT |
NGG |
2 |
0.4333 |
Tier II |
| 2 |
NC_000068.7 |
2 |
162428761 |
- |
Ptprt |
NNNGTAGGTATCCTGATCAT |
NGC |
2 |
0.0163 |
Tier II |
| 3 |
NC_000074.6 |
8 |
41213846 |
+ |
Fgl1 |
NNNGTAGATATCCTGATGAT |
NGA |
2 |
0.0093 |
Tier II |
| 4 |
NC_000068.7 |
2 |
116856782 |
- |
Gm13990 |
NNNGTAGATATCTTGATCAT |
NTG |
2 |
0.0273 |
Tier III |
| 5 |
NC_000071.6 |
5 |
63579999 |
+ |
Gm30837 |
NNNTTAGATATCCTGATCAT |
NGT |
2 |
0.0059 |
Tier III |
| 6 |
NC_000071.6 |
5 |
106573399 |
+ |
Gm8350 |
NNNGTAGAGATCCTGCTCAT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
