Construct: sgRNA BRDN0001148526
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTCCTCCACTCGAAGTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LIMK2 (3985)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75972
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
31262224 |
- |
LIMK2 |
NNNCCTCCACTCGAAGTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
13918378 |
+ |
SEL1L2 |
NNNCCTCCACTGGAAGTGTG |
NAG |
2 |
0.1152 |
Tier II |
| 3 |
NC_000014.9 |
14 |
74546914 |
- |
LTBP2 |
NNNCCTCCACTGGAAGGGTG |
NGG |
2 |
0.0523 |
Tier II |
| 4 |
NC_000005.10 |
5 |
111612456 |
+ |
STARD4-AS1 |
NNNCCGCCACTCCAAGTGTG |
NGG |
2 |
0.2406 |
Tier III |
| 5 |
NC_000007.14 |
7 |
131016720 |
+ |
LINC-PINT |
NNNCCTCCACTCTGAGTGTG |
NGG |
2 |
0.14 |
Tier III |
| 6 |
NC_000003.12 |
3 |
27712353 |
+ |
KIAA1143P2 |
NNNCCTCCACTCCAACTGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
111038485 |
+ |
Ctr9 |
NNNACTCAACTCGAAGTGTG |
NGG |
2 |
0.5474 |
Tier I |
| 2 |
NC_000072.6 |
6 |
119661665 |
- |
Erc1 |
NNNCTTCCACTCGAAATGTG |
NGG |
2 |
0.6364 |
Tier II |
| 3 |
NC_000085.6 |
19 |
5743207 |
+ |
Ltbp3 |
NNNCCTCCACTCCAAGTCTG |
NGG |
2 |
0.2005 |
Tier II |
| 4 |
NC_000067.6 |
1 |
118928535 |
+ |
Gli2 |
NNNCCTCCACTCTCAGTGTG |
NGG |
2 |
0.0643 |
Tier II |
| 5 |
NC_000083.6 |
17 |
27399001 |
+ |
Gm35007 |
NNNCCTCCACTGGAAGTGTT |
NGG |
2 |
0.3111 |
Tier III |
| 6 |
NC_000068.7 |
2 |
21112135 |
- |
Gm13377 |
NNNCCTCCACTCCAAGTGTT |
NGG |
2 |
0.2947 |
Tier III |
| 7 |
NC_000083.6 |
17 |
85714906 |
+ |
CJ186046Rik |
NNNCCTCAACTCCAAGTGTG |
NGG |
2 |
0.2737 |
Tier III |
| 8 |
NC_000071.6 |
5 |
64049962 |
+ |
5830416I19Rik |
NNNCCTCCTCTCTAAGTGTG |
NGG |
2 |
0.18 |
Tier III |
Other clones with same target sequence:
(none)
