Construct: sgRNA BRDN0001148536
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTTATACACCGTACTATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPKAPK2 (9261)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77207
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
206730094 |
+ |
MAPKAPK2 |
NNNTATACACCGTACTATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
140786369 |
- |
PTK2 |
NNNGAAACACCGTACTATGT |
NGG |
2 |
0.5417 |
Tier II |
| 3 |
NC_000010.11 |
10 |
73276869 |
- |
CFAP70 |
NNNCATACACAGTACTATGT |
NGG |
2 |
0.4853 |
Tier II |
| 4 |
NC_000013.11 |
13 |
42126863 |
- |
DGKH |
NNNTATACACAGTACTATGC |
NGG |
2 |
0.0682 |
Tier II |
| 5 |
NC_000010.11 |
10 |
73276869 |
- |
DNAJC9-AS1 |
NNNCATACACAGTACTATGT |
NGG |
2 |
0.4853 |
Tier III |
| 6 |
NC_000001.11 |
1 |
63701299 |
+ |
LOC105378771 |
NNNTATGCACCGTGCTATGT |
NGG |
2 |
0.3294 |
Tier III |
| 7 |
NC_000005.10 |
5 |
88627555 |
- |
MIR9-2HG |
NNNTATACACAGTACTATGT |
NAG |
2 |
0.1944 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
131057499 |
- |
Mapkapk2 |
NNNTATACACCATACTATGT |
NGG |
1 |
0.9333 |
Tier I |
| 2 |
NC_000076.6 |
10 |
64195296 |
- |
Ctnna3 |
NNNTATACAACGTATTATGT |
NGG |
2 |
0.0578 |
Tier II |
| 3 |
NC_000068.7 |
2 |
122002718 |
- |
Ctdspl2 |
NNNTATACACCCTACTATGT |
NTG |
2 |
0.0206 |
Tier II |
| 4 |
NC_000080.6 |
14 |
70835778 |
+ |
Gm34500 |
NNNTATACACCGTAGTATGT |
NGG |
1 |
0.05 |
Tier III |
Other clones with same target sequence:
(none)
