Construct: sgRNA BRDN0001148538

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGGGGTCAAGAACACTGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC42BPB (9578)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77777

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 102966283 - CDC42BPB NNNGGGTCAAGAACACTGGT NGG 0 1.0 Tier I
2 NC_000011.10 11 67674379 - ALDH3B2 NNNGGGTAAAGGACACTGGT NGG 2 0.4694 Tier II
3 NC_000008.11 8 95249990 - CFAP418 NNNGGGTCAAGAGCACTGAT NGG 2 0.4658 Tier II
4 NC_000001.11 1 232586884 + SIPA1L2 NNNGGGGAAAGAACACTGGT NGG 2 0.3824 Tier II
5 NC_000011.10 11 59795787 - STX3 NNNGGCTCAAGAACACAGGT NGG 2 0.3636 Tier II
6 NC_000010.11 10 21729593 + MLLT10 NNNGTGTCAAGAACACTAGT NGG 2 0.2077 Tier II
7 NC_000005.10 5 53578365 - NDUFS4 NNNGGGTCAAGGACACTGGT NAG 2 0.1872 Tier II
8 NC_000014.9 14 95509419 - SYNE3 NNNGGGTCCAGATCACTGGT NGG 2 0.1714 Tier II
9 NC_000003.12 3 174442647 - NAALADL2 NNNGTGTCAAGAACACTGCT NGG 2 0.1345 Tier II
10 NC_000005.10 5 102763384 - PAM NNNGGGTCAAGAACAGTGCT NGG 2 0.069 Tier II
11 NC_000004.12 4 6592191 + MAN2B2 NNNGGGTGAAGAACACTGGC NGG 2 0.0584 Tier II
12 NC_000019.10 19 53912760 + CACNG7 NNNGGGTCAAGCACTCTGGT NGG 2 0.0526 Tier II
13 NC_000006.12 6 155218813 - TIAM2 NNNTGGTCAAGAACACGGGT NGG 2 0.0428 Tier II
14 NC_000006.12 6 155218871 - TIAM2 NNNTGGTCAAGAACACGGGT NGG 2 0.0428 Tier II
15 NC_000006.12 6 155218900 - TIAM2 NNNTGGTCAAGAACACGGGT NGG 2 0.0428 Tier II
16 NC_000006.12 6 155218950 - TIAM2 NNNTGGTCAAGAACACGGGT NGG 2 0.0428 Tier II
17 NC_000006.12 6 155218979 - TIAM2 NNNTGGTCAAGAACACGGGT NGG 2 0.0428 Tier II
18 NC_000006.12 6 155219029 - TIAM2 NNNTGGTCAAGAACACGGGT NGG 2 0.0428 Tier II
19 NC_000006.12 6 155219058 - TIAM2 NNNTGGTCAAGAACACGGGT NGG 2 0.0428 Tier II
20 NC_000017.11 17 32304442 + RHBDL3 NNNTGGTCAAGAACACGGGT NGG 2 0.0428 Tier II
21 NC_000017.11 17 29842040 - SSH2 NNNGGGTTAAGAACACTGGT NTG 2 0.0341 Tier II
22 NC_000020.11 20 9697449 - PAK5 NNNGGGTCTAGAAGACTGGT NGG 2 0.0 Tier II
23 NC_000024.10 Y 7343111 + PRKY NNNGGGCCCAGAACACTGGT NGG 2 0.3929 Tier III
24 NC_000001.11 1 115975997 - LOC101928977 NNNGGGTTAAGTACACTGGT NGG 2 0.2917 Tier III
25 NC_000010.11 10 21729593 + LOC107984214 NNNGTGTCAAGAACACTAGT NGG 2 0.2077 Tier III
26 NC_000008.11 8 19886482 - LOC105379309 NNNGGGTCATGAACTCTGGT NGG 2 0.1765 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 4354955 + Akap12 NNNGGGCCAAGAACACTGGG NGG 2 0.1213 Tier I
2 NC_000075.6 9 41137267 + Ubash3b NNNGGGTCATGAACACTGAT NGG 2 0.6303 Tier II
3 NC_000068.7 2 27492108 - Brd3 NNNAGGTCAAGAGCACTGGT NGG 2 0.587 Tier II
4 NC_000068.7 2 155247693 - Dynlrb1 NNNGGGTTAAGAGCACTGGT NGG 2 0.5707 Tier II
5 NC_000081.6 15 9126665 - Skp2 NNNGGGTCAAGAACACTCGT NGG 1 0.4762 Tier II
6 NC_000074.6 8 68694191 - Csgalnact1 NNNGGGTCAAGAACACTTAT NGG 2 0.4762 Tier II
7 NC_000073.6 7 123043626 - Tnrc6a NNNGGCTGAAGAACACTGGT NGG 2 0.4383 Tier II
8 NC_000068.7 2 169921552 + Tshz2 NNNGGGTCAAGAAAAATGGT NGG 2 0.35 Tier II
9 NC_000067.6 1 74741984 - Prkag3 NNNTGGTTAAGAACACTGGT NGG 2 0.3182 Tier II
10 NC_000081.6 15 71540414 + Fam135b NNNGGTTCAAGAACACTGCT NGG 2 0.2989 Tier II
11 NC_000085.6 19 38686622 + Plce1 NNNGGGTAAAGAACATTGGT NGG 2 0.2 Tier II
12 NC_000073.6 7 113363137 + Btbd10 NNNGTTTCAAGAACACTGGT NGG 2 0.2 Tier II
13 NC_000071.6 5 72725003 - Txk NNNGGGTCAGGAACATTGGT NGG 2 0.1709 Tier II
14 NC_000079.6 13 102694293 - Cd180 NNNGGGTCAAGGACAGTGGT NGG 2 0.1111 Tier II
15 NC_000074.6 8 46533834 - Acsl1 NNNGGGTTAAGAACACTGGC NGG 2 0.0795 Tier II
16 NC_000069.6 3 84205180 - Trim2 NNNGGGGCAAGAACACGGGT NGG 2 0.0692 Tier II
17 NC_000071.6 5 96784588 - Fras1 NNNGGGTCAAGAACACAGGT NGA 2 0.037 Tier II
18 NC_000067.6 1 192636457 - Hhat NNNGGGTCAAGAACTCTGGG NGG 2 0.0353 Tier II
19 NC_000084.6 18 24629219 - Elp2 NNNGGGTCAAGAACACTGGC NGC 2 0.002 Tier II
20 NC_000071.6 5 144181972 + Lmtk2 NNNGGGTTAAGAAGACTGGT NGG 2 0.0 Tier II
21 NC_000077.6 11 60817430 + Gm12611 NNNGGATCAAGAACAATGGT NGG 2 1.0 Tier III
22 NC_000068.7 2 169487218 - Gm34112 NNNCGGTCTAGAACACTGGT NGG 2 0.3176 Tier III
23 NC_000068.7 2 169487218 - Gm14249 NNNCGGTCTAGAACACTGGT NGG 2 0.3176 Tier III
24 NC_000076.6 10 23698961 + Gm35291 NNNGGGTCAAGAACACTGCT NGC 2 0.01 Tier III
Download CSV

Other clones with same target sequence:

(none)

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