Construct: sgRNA BRDN0001148546
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGAGGGTATAAAGGACCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK2B (2185)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76386
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
27440359 |
- |
PTK2B |
NNNAGGGTATAAAGGACCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
41446082 |
+ |
TOB2 |
NNNAGGATATAAAGAACCGG |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000002.12 |
2 |
95026053 |
- |
MAL |
NNNAGGGCACAAAGGACCGG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000001.11 |
1 |
212978190 |
- |
VASH2 |
NNNAGGGGATGAAGGACCGG |
NGG |
2 |
0.4767 |
Tier II |
| 5 |
NC_000014.9 |
14 |
101840524 |
- |
PPP2R5C |
NNNAGGTTATAAAAGACCGG |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000011.10 |
11 |
20654232 |
- |
SLC6A5 |
NNNAGGATATAAATGACCGG |
NGG |
2 |
0.2667 |
Tier II |
| 7 |
NC_000001.11 |
1 |
53614404 |
+ |
GLIS1 |
NNNAGGGTACAAAGGACCCG |
NGG |
2 |
0.2391 |
Tier II |
| 8 |
NC_000008.11 |
8 |
4298102 |
+ |
CSMD1 |
NNNAGGGTATAAAGGACCTG |
NAG |
2 |
0.1728 |
Tier II |
| 9 |
NC_000016.10 |
16 |
5083508 |
- |
ALG1 |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000002.12 |
2 |
95026053 |
- |
LOC124906046 |
NNNAGGGCACAAAGGACCGG |
NGG |
2 |
0.5333 |
Tier III |
| 11 |
NC_000003.12 |
3 |
75420772 |
- |
ALG1L6P |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 12 |
NC_000003.12 |
3 |
125930804 |
+ |
ALG1L1P |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 13 |
NC_000004.12 |
4 |
3941145 |
- |
ALG1L7P |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 14 |
NC_000007.14 |
7 |
6929469 |
- |
ALG1L5P |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 15 |
NC_000008.11 |
8 |
12181318 |
- |
ALG1L11P |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 16 |
NC_000008.11 |
8 |
12423633 |
- |
ALG1L12P |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 17 |
NC_000011.10 |
11 |
3407448 |
- |
TSSC2 |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 18 |
NC_000011.10 |
11 |
71802027 |
+ |
ALG1L9P |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 19 |
NC_000012.12 |
12 |
8230399 |
- |
ALG1L10P |
NNNAGGGTATAAAGGCCTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
12497107 |
- |
Edaradd |
NNNAGGGTATAAAGGAACAG |
NGG |
2 |
0.3333 |
Tier II |
| 2 |
NC_000072.6 |
6 |
99179007 |
+ |
Foxp1 |
NNNAGGGTAAAAATGACCGG |
NGG |
2 |
0.2286 |
Tier II |
| 3 |
NC_000080.6 |
14 |
59682261 |
- |
Atp8a2 |
NNNAGGGTATAAAGGACCTG |
NGA |
2 |
0.0463 |
Tier II |
Other clones with same target sequence:
(none)
