Construct: sgRNA BRDN0001148547

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTAAACTGTACATCATGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C2A (5286)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76415

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 17117652 + PIK3C2A NNNAAACTGTACATCATGCA NGG 0 1.0 Tier I
2 NC_000001.11 1 171512632 + PRRC2C NNNAAACTGAAAATCATGCA NGG 2 0.6122 Tier II
3 NC_000007.14 7 39285294 - POU6F2 NNNAATCTGTGCATCATGCA NGG 2 0.4643 Tier II
4 NC_000016.10 16 3314047 + ZNF75A NNNAAAATGTATATCATGCA NGG 2 0.4038 Tier II
5 NC_000007.14 7 105994868 - CDHR3 NNNAAACAGTACTTCATGCA NGG 2 0.24 Tier II
6 NC_000001.11 1 32078983 - TMEM39B NNNAAATTGTACATCATGCA NAG 2 0.2106 Tier II
7 NC_000010.11 10 95367530 + SORBS1 NNNAAACTGTACATCATTCA NAG 2 0.1728 Tier II
8 NC_000006.12 6 65562826 - EYS NNNAAACTGTACTTCATGCA NAG 2 0.0778 Tier II
9 NC_000002.12 2 169657904 + CFAP210 NNNAAACTGTAAATCATGCA NGA 2 0.0496 Tier II
10 NC_000002.12 2 135660403 - R3HDM1 NNNAAACTTTACATCATGCA NGA 2 0.037 Tier II
11 NC_000003.12 3 14993912 + NR2C2 NNNAAACTGTACCTCAGGCA NGG 2 0.0248 Tier II
12 NC_000010.11 10 47535789 + ANXA8 NNNAAACGGTACATCATGCA NGT 2 0.0118 Tier II
13 NC_000017.11 17 48046750 + COPZ2 NNNAAACTGTACATCCTCCA NGG 2 0.0 Tier II
14 NC_000008.11 8 70341054 + NCOA2 NNNAAACTGTACATCCTGCA NGT 2 0.0 Tier II
15 NC_000023.11 X 7193430 + STS NNNAAACTGTACATTTTGCA NGG 2 0.0 Tier II
16 NC_000009.12 9 118520416 + LOC105376248 NNNAAAGTGAACATCATGCA NGG 2 0.4034 Tier III
17 NC_000016.10 16 60518892 + LOC101927605 NNNAAACTGTACATAATACA NGG 2 0.1538 Tier III
18 NC_000008.11 8 2720789 - LINC03021 NNNAAACTGCACACCATGCA NGG 2 0.1524 Tier III
19 NC_000024.10 Y 18503180 - LOC124905304 NNNAAACTGTACATCAGACA NGG 2 0.0814 Tier III
20 NC_000024.10 Y 18817227 + LOC124905305 NNNAAACTGTACATCAGACA NGG 2 0.0814 Tier III
21 NC_000005.10 5 13447477 - LOC105374660 NNNAAACTGCACATCATGCA NTG 2 0.0208 Tier III
22 NC_000010.11 10 45859704 + PARGP1 NNNAAACGGTACATCATGCA NGT 2 0.0118 Tier III
23 NC_000010.11 10 45859704 + PARGP1-AGAP4 NNNAAACGGTACATCATGCA NGT 2 0.0118 Tier III
24 NC_000010.11 10 46804153 - BMS1P1 NNNAAACGGTACATCATGCA NGT 2 0.0118 Tier III
25 NC_000010.11 10 47535789 + BMS1P2 NNNAAACGGTACATCATGCA NGT 2 0.0118 Tier III
26 NC_000010.11 10 47535789 + BMS1P2-AGAP9 NNNAAACGGTACATCATGCA NGT 2 0.0118 Tier III
27 NC_000010.11 10 49976391 - TIMM23B-AGAP6 NNNAAACGGTACATCATGCA NGT 2 0.0118 Tier III
28 NC_000004.12 4 188739245 + LOC285442 NNNAAACTGTACAGCATGCA NGT 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 24385497 - Abca3 NNNAAACAGTACATCATGCT NGG 2 0.48 Tier I
2 NC_000067.6 1 11862464 - A830018L16Rik NNNAAACTGTATATCATCCA NGG 2 0.2564 Tier II
3 NC_000069.6 3 54699615 - Supt20 NNNCAACTATACATCATGCA NGG 2 0.2269 Tier II
4 NC_000070.6 4 111557660 - Agbl4 NNNAATCTGTACACCATGCA NGG 2 0.2041 Tier II
5 NC_000074.6 8 33301228 - Wrn NNNAAACTGTGCACCATGCA NGG 2 0.1857 Tier II
6 NC_000073.6 7 16377740 - Sae1 NNNAAACTGTACATCATACA NAG 2 0.1795 Tier II
7 NC_000074.6 8 28974178 - Unc5d NNNCACCTGTACATCATGCA NGG 2 0.1604 Tier II
8 NC_000074.6 8 13887917 - Coprs NNNAAACTCTACATCGTGCA NGG 2 0.1036 Tier II
9 NC_000076.6 10 58370725 - Lims1 NNNAAACAGTACATCAGGCA NGG 2 0.0941 Tier II
10 NC_000077.6 11 48914714 + Gm12185 NNNAAACTGGACATCAGGCA NGG 2 0.0588 Tier II
11 NC_000067.6 1 124357852 - Dpp10 NNNAAACTGGACATTATGCA NGG 2 0.0333 Tier II
12 NC_000079.6 13 49883293 - Gm272 NNNATACTGTACATCATGCA NGA 2 0.0253 Tier II
13 NC_000069.6 3 100065828 - Spag17 NNNAAACTGTTCATTATGCA NGG 2 0.0205 Tier II
14 NC_000075.6 9 31437097 - Tmem45b NNNAACCTGTACATCATGCA NGT 2 0.0073 Tier II
15 NC_000071.6 5 116002917 - Cit NNNAAACTGTACACCTTGCA NGG 2 0.0 Tier II
16 NC_000086.7 X 75618758 - LOC115489104 NNNAAACTGTAGATCATGAA NGG 2 0.1905 Tier III
17 NC_000079.6 13 49883293 - Gm19011 NNNATACTGTACATCATGCA NGA 2 0.0253 Tier III
18 NC_000079.6 13 50859820 + Gm19009 NNNATACTGTACATCATGCA NGA 2 0.0253 Tier III
19 NC_000079.6 13 50859820 + Gm40947 NNNATACTGTACATCATGCA NGA 2 0.0253 Tier III
Download CSV

Other clones with same target sequence:

(none)