Construct: sgRNA BRDN0001148556

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCTCCCAGAGACCATCCCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKN1 (5585)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76277

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 14451879 + PKN1 NNNTCCCAGAGACCATCCCG NGG 0 1.0 Tier I
2 NC_000008.11 8 120802094 - SNTB1 NNNTCCCAGAGGCCATCCCG NGG 1 0.7222 Tier II
3 NC_000002.12 2 70541997 + TGFA NNNACCCAGAAACCATCCCG NGG 2 0.4762 Tier II
4 NC_000023.11 X 1496272 + P2RY8 NNNTCCCAAAGACCATCTCG NGG 2 0.4133 Tier II
5 NC_000024.10 Y 1496272 + P2RY8 NNNTCCCAAAGACCATCTCG NGG 2 0.4133 Tier II
6 NC_000005.10 5 480644 - SLC9A3 NNNTCCCAGGGGCCATCCCG NGG 2 0.4012 Tier II
7 NC_000017.11 17 81845122 + P4HB NNNTCCCAGAGACCAGCCCA NGG 2 0.3245 Tier II
8 NC_000004.12 4 997168 + IDUA NNNTCCCAGAGACCCTCCCG NGG 1 0.2727 Tier II
9 NC_000002.12 2 95126918 - ZNF514 NNNTCCCAGAACCCATCCCG NGG 2 0.2632 Tier II
10 NC_000007.14 7 1909671 + MAD1L1 NNNTCCCGGAGACCAGCCCG NGG 2 0.2538 Tier II
11 NC_000011.10 11 96205318 + MAML2 NNNTCCCAGGGACCATCCCC NGG 2 0.2381 Tier II
12 NC_000015.10 15 55527415 - LOC124903496 NNNTCCCAGAGACCATCCAC NGG 2 0.1837 Tier II
13 NC_000011.10 11 375566 - B4GALNT4 NNNACCCAGAGCCCATCCCG NGG 2 0.1253 Tier II
14 NC_000019.10 19 53537636 + ZNF331 NNNTCCCAGAGACCATCCAG NAG 2 0.1111 Tier II
15 NC_000022.11 22 44071087 + PARVB NNNTCCCAGAGAGCATCCTG NGG 2 0.0629 Tier II
16 NC_000004.12 4 75537896 + THAP6 NNNTCCCAGAGAGCATCCAG NGG 2 0.0584 Tier II
17 NC_000007.14 7 150719728 - GIMAP1 NNNTCCCAGAGACCATCCCC NGA 2 0.0298 Tier II
18 NC_000007.14 7 150719728 - GIMAP1-GIMAP5 NNNTCCCAGAGACCATCCCC NGA 2 0.0298 Tier II
19 NC_000018.10 18 52533550 + DCC NNNTCCCAGAGACCATCCAG NTG 2 0.0167 Tier II
20 NC_000019.10 19 42313888 - TMEM145 NNNTCCCAAAGACCATCCCG NGC 2 0.0143 Tier II
21 NC_000009.12 9 127451167 - RPL12 NNNTCCCAGAGACCTTCCCG NTG 2 0.0078 Tier II
22 NC_000014.9 14 24081847 - NRL NNNTCCCAGAGACGAGCCCG NGG 2 0.0 Tier II
23 NC_000005.10 5 480644 - SLC9A3-AS1 NNNTCCCAGGGGCCATCCCG NGG 2 0.4012 Tier III
24 NC_000016.10 16 50864214 - LINC02128 NNNTCCCAGACACCATTCCG NGG 2 0.2 Tier III
25 NC_000008.11 8 136843794 + LINC02055 NNNTCCCAGAGACCTTCCTG NGG 2 0.0923 Tier III
26 NC_000005.10 5 38746809 - OSMR-DT NNNTCCCAGAGAGCATCCAG NGG 2 0.0584 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 193123643 - Utp25 NNNTCCCAGAGGCCATCCCA NGG 2 0.6771 Tier I
2 NC_000075.6 9 56887323 + Cspg4 NNNTCCCAGTGACCATCCAG NGG 2 0.3782 Tier I
3 NC_000075.6 9 75894472 - Bmp5 NNNTTCCAGAGACCATCCCG NGG 1 0.6364 Tier II
4 NC_000078.6 12 73699850 - Prkch NNNTCACAGAGACCATCACG NGG 2 0.5 Tier II
5 NC_000067.6 1 89953266 - Asb18 NNNTCCCAGAGAACATCCCT NGG 2 0.2692 Tier II
6 NC_000068.7 2 104222008 - D430041D05Rik NNNTCCCAGACACCATTCCG NGG 2 0.2 Tier II
7 NC_000075.6 9 110455638 + Klhl18 NNNTCCCAGAGACCCTCCCT NGG 2 0.1909 Tier II
8 NC_000081.6 15 30381935 + Ctnnd2 NNNTCCCAGAGAACAGCCCG NGG 2 0.1331 Tier II
9 NC_000075.6 9 10290291 + Cntn5 NNNTGCCAGAGACCATCCCG NGA 2 0.0417 Tier II
10 NC_000082.6 16 50556945 - G730013B05Rik NNNTCCCAGAGTCCATCCCA NGG 2 0.3125 Tier III
11 NC_000082.6 16 5307525 - Gm41414 NNNTCCCAGAGACCCTCCCA NGG 2 0.2557 Tier III
12 NC_000074.6 8 121419986 + Gm26747 NNNCCCCAGAGACCATGCCG NGG 2 0.0381 Tier III
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Other clones with same target sequence:

(none)