Construct: sgRNA BRDN0001148559
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTATTAAACAAAGACGAGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- C8orf44-SGK3 (100533105)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75908
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
66813872 |
+ |
SGK3 |
NNNATTAAACAAAGACGAGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
66813872 |
+ |
C8orf44-SGK3 |
NNNATTAAACAAAGACGAGC |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000006.12 |
6 |
72918257 |
- |
KCNQ5 |
NNNTTTAAACAAAGACAAGC |
NGG |
2 |
0.5939 |
Tier II |
| 4 |
NC_000003.12 |
3 |
123728846 |
- |
MYLK |
NNNATTAAACAAAGACAACC |
NGG |
2 |
0.4184 |
Tier II |
| 5 |
NC_000002.12 |
2 |
169999278 |
+ |
UBR3 |
NNNATAAAACAAAGATGAGC |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000006.12 |
6 |
161042138 |
+ |
MAP3K4 |
NNNATTAAACAAAAATGAGC |
NGG |
2 |
0.2308 |
Tier II |
| 7 |
NC_000020.11 |
20 |
57368186 |
- |
RAE1 |
NNNATAAAACAAAGACCAGC |
NGG |
2 |
0.2039 |
Tier II |
| 8 |
NC_000023.11 |
X |
97240325 |
+ |
DIAPH2 |
NNNATTAAAAAAAGACCAGC |
NGG |
2 |
0.2039 |
Tier II |
| 9 |
NC_000023.11 |
X |
86358702 |
- |
DACH2 |
NNNAATAAACAAAGATGAGC |
NGG |
2 |
0.1538 |
Tier II |
| 10 |
NC_000012.12 |
12 |
101829664 |
+ |
GNPTAB |
NNNATGAAACAAAGACGAGC |
NAG |
2 |
0.1481 |
Tier II |
| 11 |
NC_000023.11 |
X |
45072075 |
- |
KDM6A |
NNNATGAAACAAAGACCAGC |
NGG |
2 |
0.1345 |
Tier II |
| 12 |
NC_000017.11 |
17 |
17692174 |
+ |
RAI1 |
NNNATTAAACAAAGAGGACC |
NGG |
2 |
0.069 |
Tier II |
| 13 |
NC_000003.12 |
3 |
15077858 |
- |
RBSN |
NNNATTAAAGAAAGAGGAGC |
NGG |
2 |
0.0598 |
Tier II |
| 14 |
NC_000001.11 |
1 |
208170323 |
+ |
PLXNA2 |
NNNATTAAACAAAGACCAGC |
NTG |
2 |
0.0092 |
Tier II |
| 15 |
NC_000007.14 |
7 |
10153180 |
+ |
LOC105375149 |
NNNATTACACAAAGAAGAGC |
NGG |
2 |
0.4286 |
Tier III |
| 16 |
NC_000009.12 |
9 |
120340296 |
- |
LOC105376253 |
NNNATTAAACAAAGACTATC |
NGG |
2 |
0.1667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
9872277 |
+ |
Sgk3 |
NNNATTAAACAAAGAAGAGC |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000081.6 |
15 |
68953716 |
+ |
Khdrbs3 |
NNNATTAAACAAAGAAGAGC |
NGG |
1 |
1.0 |
Tier II |
| 3 |
NC_000081.6 |
15 |
76506696 |
+ |
Dgat1 |
NNNAGTAAGCAAAGACGAGC |
NGG |
2 |
0.4267 |
Tier II |
| 4 |
NC_000071.6 |
5 |
92265811 |
+ |
Naaa |
NNNAATAAACAAAAACGAGC |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000080.6 |
14 |
124009410 |
+ |
Fgf14 |
NNNATTAACCAAAGAGGAGC |
NGG |
2 |
0.0879 |
Tier II |
| 6 |
NC_000080.6 |
14 |
124253799 |
+ |
Fgf14 |
NNNATTAACCAAAGAGGAGC |
NGG |
2 |
0.0879 |
Tier II |
| 7 |
NC_000086.7 |
X |
16781763 |
- |
Maob |
NNNATTAAACAAAGAAGAGC |
NTG |
2 |
0.039 |
Tier II |
| 8 |
NC_000074.6 |
8 |
103627207 |
- |
4933400L20Rik |
NNNATTCAACAAAGACGAAC |
NGG |
2 |
0.3125 |
Tier III |
| 9 |
NC_000080.6 |
14 |
124009410 |
+ |
1700024B18Rik |
NNNATTAACCAAAGAGGAGC |
NGG |
2 |
0.0879 |
Tier III |
Other clones with same target sequence:
(none)
