Construct: sgRNA BRDN0001148561

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAAAGAAATACAGTACCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPK1 (27010)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77430

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 144835560 + TPK1 NNNAAGAAATACAGTACCAG NGG 0 1.0 Tier I
2 NC_000005.10 5 139026801 + SIL1 NNNAAGAAATACAGTACCTG NTG 2 0.021 Tier I
3 NC_000002.12 2 63839896 + WDPCP NNNAAGAAACACAGTACCAA NGG 2 0.5 Tier II
4 NC_000016.10 16 10041894 - GRIN2A NNNAACAAATAAAGTACCAG NGG 2 0.487 Tier II
5 NC_000012.12 12 70254314 - CNOT2 NNNAAGAAGTACAGTACAAG NGG 2 0.359 Tier II
6 NC_000012.12 12 95918461 - CCDC38 NNNAGGAAATACAGTAACAG NGG 2 0.336 Tier II
7 NC_000001.11 1 46655537 - ATPAF1 NNNAAGAAATAAAGTAACAG NGG 2 0.3333 Tier II
8 NC_000001.11 1 62502633 + DOCK7 NNNAAGAAATAGAGTACTAG NGG 2 0.2857 Tier II
9 NC_000007.14 7 48532628 - ABCA13 NNNAACAAATCCAGTACCAG NGG 2 0.2727 Tier II
10 NC_000012.12 12 104132307 + NFYB NNNAAGAAATACAGTACTGG NGG 2 0.2411 Tier II
11 NC_000023.11 X 9577450 - TBL1X NNNAAGGAATTCAGTACCAG NGG 2 0.2172 Tier II
12 NC_000017.11 17 37120937 - ACACA NNNAACAAATTCAGTACCAG NGG 2 0.2098 Tier II
13 NC_000001.11 1 183782996 - RGL1 NNNTAGAAATTCAGTACCAG NGG 2 0.1958 Tier II
14 NC_000010.11 10 114359119 + AFAP1L2 NNNAAGAAATACATTACAAG NGG 2 0.1436 Tier II
15 NC_000003.12 3 17269059 - TBC1D5 NNNAAGAAATTCAGTATCAG NGG 2 0.1436 Tier II
16 NC_000013.11 13 48656942 - CYSLTR2 NNNAAGAAATACAGTGCTAG NGG 2 0.1236 Tier II
17 NC_000007.14 7 78805479 + MAGI2 NNNAAGAAATACAGTAGAAG NGG 2 0.0317 Tier II
18 NC_000003.12 3 101783554 - NXPE3 NNNAAGAACTACAGGACCAG NGG 2 0.0286 Tier II
19 NC_000002.12 2 157857465 + ACVR1 NNNAAGAAATACTGTACCAG NGA 2 0.0208 Tier II
20 NC_000007.14 7 55442719 + VOPP1 NNNAAGAAATACAGTGGCAG NGG 2 0.0113 Tier II
21 NC_000017.11 17 67682695 + PITPNC1 NNNAAGAAATACCGTACCAG NTG 2 0.0082 Tier II
22 NC_000019.10 19 55656211 + U2AF2 NNNAAGAAATACAGGACCAG NGA 2 0.0035 Tier II
23 NC_000008.11 8 24385583 - ADAMDEC1 NNNAAGAAATACAGTTCCAG NGA 2 0.0 Tier II
24 NC_000023.11 X 110147250 - TMEM164 NNNAAGAAATAGAGTTCCAG NGG 2 0.0 Tier II
25 NC_000014.9 14 30486015 + G2E3-AS1 NNNAAAAAAAACAGTACCAG NGG 2 0.8571 Tier III
26 NC_000020.11 20 60309001 - MIR646HG NNNAAGAAATGCAGTGCCAG NGG 2 0.125 Tier III
27 NC_000008.11 8 25672671 + LOC105379333 NNNAAGAAACACAGTGCCAG NGG 2 0.1026 Tier III
28 NC_000008.11 8 25672671 + LOC107986933 NNNAAGAAACACAGTGCCAG NGG 2 0.1026 Tier III
29 NC_000001.11 1 187350315 + LINC01036 NNNAAGAAATACAGTATCAG NGA 2 0.0324 Tier III
30 NC_000023.11 X 74235196 + FTX NNNAAGAATTACAGTACCAG NGT 2 0.0097 Tier III
31 NC_000017.11 17 67682695 + LOC101928045 NNNAAGAAATACCGTACCAG NTG 2 0.0082 Tier III
32 NC_000008.11 8 24385583 - ADAM7-AS1 NNNAAGAAATACAGTTCCAG NGA 2 0.0 Tier III
33 NC_000004.12 4 129312575 - LOC105377416 NNNAAGAAATACAGTTCTAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 94807258 - Tek NNNAAGAAAAACAATACCAG NGG 2 0.6429 Tier II
2 NC_000073.6 7 46569392 - Sergef NNNAAGGAATACAGTACCAT NGG 2 0.4941 Tier II
3 NC_000070.6 4 88195726 + Focad NNNTAGAAATACAATACCAG NGG 2 0.4773 Tier II
4 NC_000078.6 12 108657790 - Evl NNNAAAAAATACAGTAACAG NGG 2 0.4667 Tier II
5 NC_000069.6 3 151503225 + Adgrl4 NNNAAGAAACACAGTACCAT NGG 2 0.3733 Tier II
6 NC_000068.7 2 126388830 - Atp8b4 NNNAAGAGAGACAGTACCAG NGG 2 0.3667 Tier II
7 NC_000070.6 4 107269143 + Hspb11 NNNAAGAAGTATAGTACCAG NGG 2 0.359 Tier II
8 NC_000067.6 1 174504378 - Fmn2 NNNAAGGAAGACAGTACCAG NGG 2 0.3529 Tier II
9 NC_000077.6 11 52784691 + Fstl4 NNNAGGAAATAGAGTACCAG NGG 2 0.32 Tier II
10 NC_000073.6 7 122552090 + Prkcb NNNAACAAATACACTACCAG NGG 2 0.2922 Tier II
11 NC_000072.6 6 144770394 + Sox5 NNNAAGAAACACAGTACCTG NGG 2 0.2872 Tier II
12 NC_000078.6 12 26309042 + Rnf144a NNNAAGAAAATCAGTACCAG NGG 2 0.2637 Tier II
13 NC_000083.6 17 84721689 + Lrpprc NNNAAGATATACAGTACAAG NGG 2 0.2308 Tier II
14 NC_000070.6 4 115607686 + Cyp4a32 NNNAAGAAATACTGAACCAG NGG 2 0.1737 Tier II
15 NC_000081.6 15 57445010 - Slc22a22 NNNAAGAAAGACAGTACCAG NAG 2 0.1296 Tier II
16 NC_000076.6 10 70565331 + Phyhipl NNNAAGCAATACAGTACCAG NAG 2 0.1134 Tier II
17 NC_000074.6 8 4522808 + Cers4 NNNAAGAAATAAAGTAGCAG NGG 2 0.042 Tier II
18 NC_000079.6 13 92248017 + Msh3 NNNAAGAAATAAAGTAGCAG NGG 2 0.042 Tier II
19 NC_000070.6 4 35023478 + Mob3b NNNAAAAAATACAGTACCAG NTG 2 0.039 Tier II
20 NC_000070.6 4 97735996 - Nfia NNNAACAAATACAGGACCAG NGG 2 0.0341 Tier II
21 NC_000072.6 6 73185036 - Dnah6 NNNAACAAATACAGGACCAG NGG 2 0.0341 Tier II
22 NC_000068.7 2 109168988 + Mettl15 NNNAAGAAAAACAGTACCAG NTG 2 0.0334 Tier II
23 NC_000071.6 5 53637803 + Rbpj NNNAAGAAATACAGGACCAC NGG 2 0.0214 Tier II
24 NC_000070.6 4 102096215 + Pde4b NNNAAGAAATACTGTAGCAG NGG 2 0.0176 Tier II
25 NC_000069.6 3 106716185 - Lrif1 NNNAAGAAATACAGGACCAG NCG 2 0.0054 Tier II
26 NC_000077.6 11 80196009 - Rnf135 NNNAACAAATACAGTTCCAG NGG 2 0.0 Tier II
27 NC_000078.6 12 111828667 + Ppp1r13b NNNAAGAAACACAGTTCCAG NGG 2 0.0 Tier II
28 NC_000083.6 17 80237580 - Gm52289 NNNAAGAAACAGAGTACCAG NGG 2 0.237 Tier III
29 NC_000076.6 10 66163720 + 1700023F02Rik NNNAAGCAATACAGTACCTG NGG 2 0.2356 Tier III
30 NC_000076.6 10 66163720 + 4930407I19Rik NNNAAGCAATACAGTACCTG NGG 2 0.2356 Tier III
31 NC_000083.6 17 29465342 - Gm36199 NNNAAGAAATACATTACCGG NGG 2 0.1 Tier III
32 NC_000083.6 17 29465342 - Gm26885 NNNAAGAAATACATTACCGG NGG 2 0.1 Tier III
33 NC_000068.7 2 21069832 + Gm13377 NNNAAGAAATAAAGGACCAG NGG 2 0.0357 Tier III
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Other clones with same target sequence:

(none)