Construct: sgRNA BRDN0001148563
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGTGATCACACTCCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TGFBR2 (7048)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77467
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
30672285 |
+ |
TGFBR2 |
NNNGTGATCACACTCCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
2658155 |
- |
TTYH3 |
NNNGTGTGCACACTCCATGT |
NGG |
2 |
0.3208 |
Tier II |
| 3 |
NC_000002.12 |
2 |
174237247 |
- |
OLA1 |
NNNATGATCAGACTCCATGT |
NGG |
2 |
0.225 |
Tier II |
| 4 |
NC_000018.10 |
18 |
58941518 |
- |
ZNF532 |
NNNGTGATCACACTCCAGTT |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000014.9 |
14 |
93186720 |
- |
LYSET |
NNNGTGATCAGACTCCATAT |
NGG |
2 |
0.1786 |
Tier II |
| 6 |
NC_000016.10 |
16 |
12572308 |
+ |
SNX29 |
NNNGTGATCACAATCCAGGT |
NGG |
2 |
0.1282 |
Tier II |
| 7 |
NC_000006.12 |
6 |
56370167 |
- |
COL21A1 |
NNNGAGATCAGACTCCATGT |
NGG |
2 |
0.125 |
Tier II |
| 8 |
NC_000010.11 |
10 |
12448820 |
- |
CAMK1D |
NNNGTGATCACACTCCATTT |
NCG |
2 |
0.0714 |
Tier II |
| 9 |
NC_000004.12 |
4 |
9826763 |
- |
SLC2A9 |
NNNGTGATAACACTTCATGT |
NGG |
2 |
0.0571 |
Tier II |
| 10 |
NC_000005.10 |
5 |
75456637 |
+ |
CERT1 |
NNNGAGATCACACTCCATGC |
NGG |
2 |
0.0455 |
Tier II |
| 11 |
NC_000017.11 |
17 |
55811783 |
+ |
PCTP |
NNNGTGATCACACTCCTTGT |
NAG |
2 |
0.0346 |
Tier II |
| 12 |
NC_000002.12 |
2 |
149150079 |
- |
LYPD6B |
NNNGTGATCACAGTACATGT |
NGG |
2 |
0.0303 |
Tier II |
| 13 |
NC_000005.10 |
5 |
72997865 |
+ |
FCHO2 |
NNNGTGTTCACACTCCATGT |
NTG |
2 |
0.017 |
Tier II |
| 14 |
NC_000011.10 |
11 |
18776204 |
+ |
PTPN5 |
NNNGTGATCACACTTCTTGT |
NGG |
2 |
0.0089 |
Tier II |
| 15 |
NC_000014.9 |
14 |
81738524 |
- |
LINC02311 |
NNNGTGGTCACACTCTATGT |
NGG |
2 |
0.2172 |
Tier III |
| 16 |
NC_000014.9 |
14 |
81738524 |
- |
LOC107984704 |
NNNGTGGTCACACTCTATGT |
NGG |
2 |
0.2172 |
Tier III |
| 17 |
NC_000016.10 |
16 |
12572308 |
+ |
LOC105371085 |
NNNGTGATCACAATCCAGGT |
NGG |
2 |
0.1282 |
Tier III |
| 18 |
NC_000013.11 |
13 |
80162061 |
- |
LOC105370275 |
NNNGTGATCAGAATCCATGT |
NGG |
2 |
0.0962 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
116109657 |
- |
Tgfbr2 |
NNNGTGACCACACTCCTTGT |
NGG |
2 |
0.1333 |
Tier I |
| 2 |
NC_000077.6 |
11 |
43557445 |
+ |
Ccnjl |
NNNGTGAGCACACTCCATAT |
NGG |
2 |
0.5238 |
Tier II |
| 3 |
NC_000070.6 |
4 |
114613268 |
- |
Trabd2b |
NNNGTGACCACACTCCATGT |
NAG |
2 |
0.2593 |
Tier II |
| 4 |
NC_000073.6 |
7 |
29505329 |
+ |
Sipa1l3 |
NNNGTGATCACACTCCAGGA |
NGG |
2 |
0.1875 |
Tier II |
| 5 |
NC_000084.6 |
18 |
31689702 |
+ |
Sap130 |
NNNGTGAACACACTCCATGT |
NGT |
2 |
0.0129 |
Tier II |
| 6 |
NC_000069.6 |
3 |
146002862 |
- |
Syde2 |
NNNGTGATCACACGCCAAGT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000070.6 |
4 |
114613268 |
- |
Gm33655 |
NNNGTGACCACACTCCATGT |
NAG |
2 |
0.2593 |
Tier III |
| 8 |
NC_000074.6 |
8 |
57337707 |
+ |
Hdnr |
NNNTTGATCACACACCATGT |
NGG |
2 |
0.2251 |
Tier III |
| 9 |
NC_000080.6 |
14 |
52837733 |
- |
Tcra |
NNNGTGATCACACTCTATGC |
NGG |
2 |
0.028 |
Tier III |
| 10 |
NC_000080.6 |
14 |
53148794 |
- |
Tcra |
NNNGTGATCACACTCTATGC |
NGG |
2 |
0.028 |
Tier III |
| 11 |
NC_000080.6 |
14 |
53532658 |
- |
Tcra |
NNNGTGATCACACTCTATGC |
NGG |
2 |
0.028 |
Tier III |
| 12 |
NC_000082.6 |
16 |
93408630 |
- |
1700029J03Rik |
NNNTTGATCACACTCCATGT |
NGT |
2 |
0.0059 |
Tier III |
| 13 |
NC_000069.6 |
3 |
146002862 |
- |
Gm40166 |
NNNGTGATCACACGCCAAGT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
