Construct: sgRNA BRDN0001148564

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCATGAAGAGAGAACTACCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WEE1 (7465)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77369

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 9575941 - WEE1 NNNTGAAGAGAGAACTACCC NGG 0 1.0 Tier I
2 NC_000008.11 8 86222987 + SLC7A13 NNNTGAAGAGAGAATTTCCC NGG 2 0.0089 Tier I
3 NC_000012.12 12 86087206 - MGAT4C NNNTGGAGATAGAACTACCC NGG 2 0.5804 Tier II
4 NC_000012.12 12 68894659 - CPM NNNTGAAGAGAGAAAAACCC NGG 2 0.202 Tier II
5 NC_000007.14 7 93195552 - HEPACAM2 NNNTGAAGAGAGAACTAACT NGG 2 0.1615 Tier II
6 NC_000003.12 3 4035680 + SUMF1 NNNTGAAGACAGACCTACCC NGG 2 0.0857 Tier II
7 NC_000017.11 17 12091064 + MAP2K4 NNNTGAAGAGAGAGCTCCCC NGG 2 0.0824 Tier II
8 NC_000014.9 14 95306231 + CLMN NNNAGAAGAGAGAACTAGCC NGG 2 0.0635 Tier II
9 NC_000001.11 1 85044763 + MCOLN3 NNNTGAAGAGAGAAATTCCC NGG 2 0.0296 Tier II
10 NC_000007.14 7 43353817 + HECW1 NNNTGAAGAGAGAACTATCC NTG 2 0.025 Tier II
11 NC_000007.14 7 148071883 - CNTNAP2 NNNTGAAGAGTGAACTACCC NTG 2 0.012 Tier II
12 NC_000014.9 14 23155519 - SLC7A8 NNNTGAAGAGAGAACTACTC NGC 2 0.0103 Tier II
13 NC_000006.12 6 158989663 - RSPH3 NNNTGAAGAGAGAAATACCC NTG 2 0.0087 Tier II
14 NC_000009.12 9 115096791 + TNC NNNTGAAGAGATAACTACCC NGT 2 0.0062 Tier II
15 NC_000016.10 16 65402951 + LINC00922 NNNTTAAAAGAGAACTACCC NGG 2 0.3 Tier III
16 NC_000009.12 9 26499390 - LOC105375999 NNNTGAAGAGAAAACTACCC NAG 2 0.242 Tier III
17 NC_000003.12 3 4035680 + LOC102723512 NNNTGAAGACAGACCTACCC NGG 2 0.0857 Tier III
18 NC_000009.12 9 115096791 + LOC124902255 NNNTGAAGAGATAACTACCC NGT 2 0.0062 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 110124509 - Wee1 NNNTGAATAGAGAACTACCC NGG 1 0.625 Tier I
2 NC_000082.6 16 5240640 - Alg1 NNNGGAAGAGAGAACTACCC NGG 1 0.625 Tier II
3 NC_000073.6 7 92897827 - Prcp NNNTCAGGAGAGAACTACCC NGG 2 0.5546 Tier II
4 NC_000072.6 6 12435863 - Thsd7a NNNTGAAGAGAGAACAGCCC NGG 2 0.1604 Tier II
5 NC_000069.6 3 28085464 - Pld1 NNNTAAAGAGAGAACTCCCC NGG 2 0.1529 Tier II
6 NC_000071.6 5 92902908 + Shroom3 NNNTAAAGAGAGAACTAGCC NGG 2 0.1156 Tier II
7 NC_000072.6 6 42668912 - Tcaf1 NNNAGAAGAGAGCACTACCC NGG 2 0.1003 Tier II
8 NC_000070.6 4 19824633 - Slc7a13 NNNTGAAGAGAGAACTTTCC NGG 2 0.0857 Tier II
9 NC_000075.6 9 119676236 - Scn10a NNNGGAAGAGAGAACTTCCC NGG 2 0.0833 Tier II
10 NC_000067.6 1 158354603 + Brinp2 NNNTGAAGAGAGAACTCCTC NGG 2 0.0814 Tier II
11 NC_000069.6 3 98636701 - Hsd3b5 NNNTGAAGACAGAACTACCC NGA 2 0.0278 Tier II
12 NC_000067.6 1 125943784 + Nckap5 NNNTGAAGAGAGAAGGACCC NGG 2 0.0173 Tier II
13 NC_000073.6 7 114835406 - Insc NNNTGAAGAGAGAATTACCC NAG 2 0.0173 Tier II
14 NC_000070.6 4 124731680 - Sf3a3 NNNTGAAGAGAGAACTACCT NTG 2 0.0117 Tier II
15 NC_000068.7 2 155064665 - Ahcy NNNTGAAGGGAGAACTACCC NGT 2 0.0108 Tier II
16 NC_000068.7 2 49042515 - Mbd5 NNNTGAAGAGAGAACTAGCC NGA 2 0.0093 Tier II
17 NC_000080.6 14 88726296 + Gm41225 NNNAGAAGAGAGAACTACAC NGG 2 0.2041 Tier III
18 NC_000069.6 3 57973602 - Gm32753 NNNTGAAGAGAGAAACACCC NGG 2 0.1481 Tier III
19 NC_000069.6 3 57973602 - Gm40055 NNNTGAAGAGAGAAACACCC NGG 2 0.1481 Tier III
20 NC_000072.6 6 133068360 + Gm35765 NNNTGAAGAGACAAATACCC NGG 2 0.1176 Tier III
21 NC_000080.6 14 123059155 - Gm33906 NNNTGAAGACAGACCTACCC NGG 2 0.0857 Tier III
22 NC_000067.6 1 156216300 - Gm30472 NNNTGAACAGAGAACTACCC NGA 2 0.0427 Tier III
23 NC_000072.6 6 35991672 - Gm38790 NNNTGAAGAGATAACTACCC NGA 2 0.0267 Tier III
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Other clones with same target sequence:

(none)