Construct: sgRNA BRDN0001148567

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTACCATTTGGCTTGCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PACSIN1 (29993)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77156

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 34529434 + PACSIN1 NNNCCATTTGGCTTGCAAAG NGG 0 1.0 Tier I
2 NC_000013.11 13 45151785 + GTF2F2 NNNCCATTTGTCTTGCAAAG NGT 2 0.0062 Tier I
3 NC_000003.12 3 64240415 - PRICKLE2 NNNCCATTTAGCCTGCAAAG NGG 2 0.7368 Tier II
4 NC_000022.11 22 30706744 - OSBP2 NNNACATGTGGCTTGCAAAG NGG 2 0.6175 Tier II
5 NC_000011.10 11 131722352 + NTM NNNCTATTTGGCTTGCAAAA NGG 2 0.5966 Tier II
6 NC_000011.10 11 83785863 - DLG2 NNNCCATATGGCTAGCAAAG NGG 2 0.4952 Tier II
7 NC_000008.11 8 89977867 + NBN NNNCTATGTGGCTTGCAAAG NGG 2 0.4667 Tier II
8 NC_000001.11 1 14740079 + KAZN NNNCCATCTGCCTTGCAAAG NGG 2 0.4286 Tier II
9 NC_000003.12 3 42769407 - CCDC13 NNNCCAATTGTCTTGCAAAG NGG 2 0.3365 Tier II
10 NC_000001.11 1 41637010 + HIVEP3 NNNCCATTTGGTTAGCAAAG NGG 2 0.3333 Tier II
11 NC_000005.10 5 131999358 - ACSL6 NNNCCCTTTGGCTTGCAAAG NGA 2 0.0316 Tier II
12 NC_000003.12 3 1142639 - CNTN6 NNNCCATATGGCTTGCAAAG NTG 2 0.0312 Tier II
13 NC_000022.11 22 23226738 + BCR NNNCCATGTGGCTTGCAAAG NTG 2 0.0286 Tier II
14 NC_000019.10 19 4214973 - ANKRD24 NNNCCATTTGGCATGCAAAG NGC 2 0.0154 Tier II
15 NC_000007.14 7 129012799 - TNPO3 NNNCCATATGGCTTGCAAAG NGT 2 0.0129 Tier II
16 NC_000022.11 22 46685662 + CERK NNNCCATTTGGCTTGCACAG NGC 2 0.0042 Tier II
17 NC_000005.10 5 125213140 - LOC101927421 NNNCAATTTGGCTTGCAAAT NGG 2 0.4 Tier III
18 NC_000012.12 12 54097411 - FLJ12825 NNNTCATTTGGCTTGTAAAG NGG 2 0.2462 Tier III
19 NC_000012.12 12 95655656 - PGAM1P5 NNNTCATTTGGCTTCCAAAG NGG 2 0.2182 Tier III
20 NC_000002.12 2 18907455 + LOC105373456 NNNCCATTTGGGTTGCAGAG NGG 2 0.1778 Tier III
21 NC_000007.14 7 92907706 + CDK6-AS1 NNNCCATTTGTCTTCCAAAG NGG 2 0.1049 Tier III
22 NC_000014.9 14 53783425 - LINC02331 NNNCCATATGGCTTGCAAAG NGA 2 0.0556 Tier III
23 NC_000001.11 1 170219454 + LINC01681 NNNCCATATGGCTTGCAAAG NTG 2 0.0312 Tier III
24 NC_000006.12 6 104472428 + LOC105377918 NNNCCATTTGGCCTGCAAAG NTG 2 0.0308 Tier III
25 NC_000007.14 7 149370954 + LOC124901770 NNNCCATTTGGCTTGCAAGG NTG 2 0.0146 Tier III
26 NC_000017.11 17 68852247 - LOC105371874 NNNCCATTTGGCTGCCAAAG NGG 2 0.0 Tier III
27 NC_000010.11 10 64077059 - LOC124902439 NNNCCATTTGGCTTGCAAAG NCA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 75995468 - Gtf2f2 NNNCCATTTGTCTTGCAAAG NGT 2 0.0062 Tier I
2 NC_000074.6 8 121085000 + Foxf1 NNNCCATTTGGCTGGCAACG NGG 2 0.0 Tier I
3 NC_000072.6 6 92500357 - Prickle2 NNNTCATTTGGCTTGCAAAA NGG 2 0.75 Tier II
4 NC_000077.6 11 49832553 + Gfpt2 NNNCCATGTGACTTGCAAAG NGG 2 0.7333 Tier II
5 NC_000071.6 5 62712957 - Arap2 NNNCCTCTTGGCTTGCAAAG NGG 2 0.4911 Tier II
6 NC_000067.6 1 20510979 - Pkhd1 NNNCCAGTTGGCTTGCAAAT NGG 2 0.4118 Tier II
7 NC_000072.6 6 32836761 + Chchd3 NNNCCATTTGGCATGCAATG NGG 2 0.3728 Tier II
8 NC_000074.6 8 58070041 - Galntl6 NNNCCATCTGGCTTGCAAAG NAG 2 0.2593 Tier II
9 NC_000068.7 2 179642127 + Cdh4 NNNCCCTTTGGGTTGCAAAG NGG 2 0.202 Tier II
10 NC_000075.6 9 47830068 - Cadm1 NNNCTATTTGGCTTGCCAAG NGG 2 0.1123 Tier II
11 NC_000083.6 17 62610079 - Efna5 NNNCCATTTGGCTTCTAAAG NGG 2 0.0839 Tier II
12 NC_000070.6 4 18807622 + Cnbd1 NNNCCATTTTGCTTGCAAAG NTG 2 0.0317 Tier II
13 NC_000078.6 12 74059845 + Syt16 NNNCCATATGGCTTGCAAAG NTG 2 0.0312 Tier II
14 NC_000086.7 X 167475888 - Frmpd4 NNNTCATTTGGCTTGCAAAG NGT 2 0.0129 Tier II
15 NC_000074.6 8 70800918 + Mast3 NNNCCACTTGGCTGGCAAAG NGG 2 0.0 Tier II
16 NC_000086.7 X 167719807 - Frmpd4 NNNCCATTTGGCTGGCAAAG NTG 2 0.0 Tier II
17 NC_000071.6 5 96258459 + Mrpl1 NNNCCATTTGGCTTGCAAAG NCA 2 0.0 Tier II
18 NC_000077.6 11 49832553 + Gm36507 NNNCCATGTGACTTGCAAAG NGG 2 0.7333 Tier III
19 NC_000069.6 3 151872862 + Gm31596 NNNGCATTTGGCTTGCAAGG NGG 2 0.1875 Tier III
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Other clones with same target sequence:

(none)