Construct: sgRNA BRDN0001148568
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAAATTGGCGTGTTAGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK3 (50808)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76585
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
4722611 |
- |
AK3 |
NNNAATTGGCGTGTTAGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
23129346 |
+ |
KLHL7 |
NNNAATTGGTATGTTAGCCA |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000012.12 |
12 |
128944781 |
- |
GLT1D1 |
NNNAATTGACGTGTTAGCCA |
NGG |
1 |
0.6429 |
Tier II |
| 4 |
NC_000002.12 |
2 |
96502412 |
- |
NEURL3 |
NNNAATGGGGGTGTTAGCCA |
NGG |
2 |
0.2288 |
Tier II |
| 5 |
NC_000007.14 |
7 |
23129346 |
+ |
AK3P3 |
NNNAATTGGTATGTTAGCCA |
NGG |
2 |
0.9412 |
Tier III |
| 6 |
NC_000012.12 |
12 |
128944781 |
- |
AK3P6 |
NNNAATTGACGTGTTAGCCA |
NGG |
1 |
0.6429 |
Tier III |
| 7 |
NC_000002.12 |
2 |
23375265 |
+ |
LINC02923 |
NNNAATTGCCATGTTAGCCA |
NGG |
2 |
0.5385 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
16026880 |
- |
Inhba |
NNNAATTGGCGTGATAGCCA |
NAG |
2 |
0.1605 |
Tier I |
| 2 |
NC_000070.6 |
4 |
116782692 |
- |
Tesk2 |
NNNAATAGGCGTGTTAGCCA |
NGG |
1 |
0.875 |
Tier II |
| 3 |
NC_000075.6 |
9 |
55804871 |
- |
Scaper |
NNNAATTGGTGTATTAGCCA |
NGG |
2 |
0.8688 |
Tier II |
| 4 |
NC_000075.6 |
9 |
106818789 |
+ |
Dcaf1 |
NNNAGTTGGTGTGTTAGCCA |
NGG |
2 |
0.6776 |
Tier II |
| 5 |
NC_000084.6 |
18 |
12769560 |
- |
Osbpl1a |
NNNAATTGGTGTCTTAGCCA |
NGG |
2 |
0.3963 |
Tier II |
| 6 |
NC_000076.6 |
10 |
125343750 |
- |
Slc16a7 |
NNNAATGGGCGTGTTAGCCT |
NGG |
2 |
0.3529 |
Tier II |
| 7 |
NC_000076.6 |
10 |
92338911 |
+ |
Gm20757 |
NNNAATTGGCATGTTAGCCT |
NGG |
2 |
0.6 |
Tier III |
Other clones with same target sequence:
(none)
