Construct: sgRNA BRDN0001148578
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTACTACTGTAGCCAACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R1 (5295)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77092
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
68292299 |
+ |
PIK3R1 |
NNNACTACTGTAGCCAACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
61787957 |
+ |
PATJ |
NNNCCTACTGTAGCCAGCAA |
NGG |
2 |
0.0623 |
Tier I |
| 3 |
NC_000012.12 |
12 |
112321427 |
+ |
HECTD4 |
NNNACTACTGTAATCAACAA |
NGG |
2 |
0.6769 |
Tier II |
| 4 |
NC_000002.12 |
2 |
68385085 |
- |
PLEK |
NNNACAAATGTAGCCAACAA |
NGG |
2 |
0.5633 |
Tier II |
| 5 |
NC_000003.12 |
3 |
133366843 |
- |
TMEM108 |
NNNACTACTGAAGTCAACAA |
NGG |
2 |
0.55 |
Tier II |
| 6 |
NC_000008.11 |
8 |
101199782 |
- |
ZNF706 |
NNNACTACTGTAGCCAAAAA |
NGG |
1 |
0.5385 |
Tier II |
| 7 |
NC_000003.12 |
3 |
48998208 |
- |
P4HTM |
NNNACTGCTGTGGCCAACAA |
NGG |
2 |
0.5098 |
Tier II |
| 8 |
NC_000009.12 |
9 |
9174638 |
+ |
PTPRD |
NNNACAACTGTTGCCAACAA |
NGG |
2 |
0.2889 |
Tier II |
| 9 |
NC_000007.14 |
7 |
18293127 |
- |
HDAC9 |
NNNAGTACTGTAGCCAACGA |
NGG |
2 |
0.225 |
Tier II |
| 10 |
NC_000002.12 |
2 |
31393113 |
- |
XDH |
NNNACTACTTTAGCCAACAA |
NAG |
2 |
0.2106 |
Tier II |
| 11 |
NC_000003.12 |
3 |
113268147 |
+ |
BOC |
NNNACTCCTGTAGACAACAA |
NGG |
2 |
0.1531 |
Tier II |
| 12 |
NC_000002.12 |
2 |
77287947 |
+ |
LRRTM4 |
NNNACTACTGTATACAACAA |
NGG |
2 |
0.105 |
Tier II |
| 13 |
NC_000014.9 |
14 |
71387535 |
+ |
SIPA1L1 |
NNNATTACTGTAGCCAAGAA |
NGG |
2 |
0.0848 |
Tier II |
| 14 |
NC_000009.12 |
9 |
23690344 |
- |
ELAVL2 |
NNNACTACTGTAGCAAACCA |
NGG |
2 |
0.046 |
Tier II |
| 15 |
NC_000009.12 |
9 |
109175750 |
+ |
EPB41L4B |
NNNTCTACTGTAGCCAACAA |
NGC |
2 |
0.0141 |
Tier II |
| 16 |
NC_000007.14 |
7 |
70303616 |
- |
AUTS2 |
NNNACTACTGTAACCCACAA |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000003.12 |
3 |
133366843 |
- |
LOC101927432 |
NNNACTACTGAAGTCAACAA |
NGG |
2 |
0.55 |
Tier III |
| 18 |
NC_000003.12 |
3 |
48998208 |
- |
LOC124906235 |
NNNACTGCTGTGGCCAACAA |
NGG |
2 |
0.5098 |
Tier III |
| 19 |
NC_000007.14 |
7 |
108825813 |
- |
LOC107986836 |
NNNACTACTGAAGACAACAA |
NGG |
2 |
0.2625 |
Tier III |
| 20 |
NC_000007.14 |
7 |
119671003 |
- |
LINC02476 |
NNNACAACTGTAGCCAACAA |
NAG |
2 |
0.2247 |
Tier III |
| 21 |
NC_000003.12 |
3 |
113268147 |
+ |
LOC124909409 |
NNNACTCCTGTAGACAACAA |
NGG |
2 |
0.1531 |
Tier III |
| 22 |
NC_000005.10 |
5 |
159840311 |
+ |
LINC01847 |
NNNATTACTGTAGCCAACAA |
NGT |
2 |
0.0103 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
101690544 |
- |
Pik3r1 |
NNNACTACTGTAGCCAACAA |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000079.6 |
13 |
57762616 |
+ |
Spock1 |
NNNACAATTGTAGCCAACAA |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000070.6 |
4 |
63130100 |
+ |
Zfp618 |
NNNAGTACTGCAGCCAACAA |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000070.6 |
4 |
102331489 |
+ |
Pde4b |
NNNAATACTGTAGCCAATAA |
NGG |
2 |
0.3673 |
Tier II |
| 5 |
NC_000079.6 |
13 |
68902222 |
+ |
Adcy2 |
NNNACTTCTGAAGCCAACAA |
NGG |
2 |
0.3281 |
Tier II |
| 6 |
NC_000074.6 |
8 |
14205156 |
- |
Dlgap2 |
NNNGCTCCTGTAGCCAACAA |
NGG |
2 |
0.2734 |
Tier II |
| 7 |
NC_000079.6 |
13 |
109459822 |
- |
Pde4d |
NNNACTACTCTAGCCAAAAA |
NGG |
2 |
0.2154 |
Tier II |
| 8 |
NC_000070.6 |
4 |
88403512 |
+ |
Focad |
NNNACTGCTGTAGCCAACAC |
NGG |
2 |
0.1604 |
Tier II |
| 9 |
NC_000068.7 |
2 |
166951927 |
+ |
Stau1 |
NNNACTGCTGTAGCCAGCAA |
NGG |
2 |
0.1246 |
Tier II |
| 10 |
NC_000068.7 |
2 |
84948888 |
- |
Slc43a3 |
NNNTCTACTGTAGCTAACAA |
NGG |
2 |
0.0424 |
Tier II |
| 11 |
NC_000073.6 |
7 |
125663834 |
- |
Gtf3c1 |
NNNACTACTGTAGCCAAGAA |
NAG |
2 |
0.0346 |
Tier II |
| 12 |
NC_000075.6 |
9 |
110106721 |
+ |
Dhx30 |
NNNACTACTGTAGCCAAGAC |
NGG |
2 |
0.0303 |
Tier II |
| 13 |
NC_000079.6 |
13 |
74376999 |
- |
Zfp72 |
NNNACTACAGTAGCCAACAA |
NGC |
2 |
0.0206 |
Tier II |
| 14 |
NC_000080.6 |
14 |
61182051 |
- |
Sacs |
NNNACTCCTGTAGCCAACAA |
NTG |
2 |
0.017 |
Tier II |
| 15 |
NC_000072.6 |
6 |
91224051 |
- |
Fbln2 |
NNNACAACTGTAGGCAACAA |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000083.6 |
17 |
65753112 |
- |
Rab31 |
NNNACTATTGTAGCCCACAA |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000070.6 |
4 |
102331489 |
+ |
Gm52669 |
NNNAATACTGTAGCCAATAA |
NGG |
2 |
0.3673 |
Tier III |
| 18 |
NC_000071.6 |
5 |
66172059 |
+ |
1700126H18Rik |
NNNACTACTGCAGCCAGCAA |
NGG |
2 |
0.1176 |
Tier III |
| 19 |
NC_000071.6 |
5 |
66172059 |
+ |
Gm26725 |
NNNACTACTGCAGCCAGCAA |
NGG |
2 |
0.1176 |
Tier III |
| 20 |
NC_000078.6 |
12 |
80037854 |
+ |
Gm36660 |
NNNACTACTGTAGGCAAGAA |
NGG |
2 |
0.0 |
Tier III |
| 21 |
NC_000078.6 |
12 |
80037854 |
+ |
Gm36834 |
NNNACTACTGTAGGCAAGAA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
