Construct: sgRNA BRDN0001148579
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGGAGCCGAACATATCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K19 (80122)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76694
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
134986812 |
+ |
MAP3K19 |
NNNGAGCCGAACATATCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
24804526 |
- |
TNRC6A |
NNNGAGCCAAACATATTTCA |
NGG |
2 |
0.3 |
Tier II |
| 3 |
NC_000003.12 |
3 |
139627405 |
- |
NMNAT3 |
NNNGAGCTGAACACATCTCA |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000020.11 |
20 |
32655045 |
+ |
C20orf203 |
NNNGAGCCGAACATATTTAA |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000008.11 |
8 |
48428844 |
- |
LOC105375821 |
NNNGTGCCGAACATCTCTCA |
NGG |
2 |
0.0992 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
127822962 |
+ |
Map3k19 |
NNNGAGCCGTACGTATCTCA |
NGG |
2 |
0.5754 |
Tier I |
| 2 |
NC_000083.6 |
17 |
11325198 |
+ |
Prkn |
NNNGAACCCAACATATCTCA |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000068.7 |
2 |
3655741 |
+ |
LOC108168726 |
NNNGAGCCAAGCATATCTCA |
NGG |
2 |
0.4179 |
Tier II |
Other clones with same target sequence:
(none)
