Construct: sgRNA BRDN0001148584
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAGGCGAGTAATACCTGTC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK14 (1432)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77922
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
36075961 |
- |
MAPK14 |
NNNGGCGAGTAATACCTGTC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
9449072 |
+ |
LOC408186 |
NNNGGCGAGTAATACCCTTC |
NGG |
2 |
0.4706 |
Tier III |
| 3 |
NC_000024.10 |
Y |
11132878 |
+ |
MTCO3P37 |
NNNGGGGAGAAATACCTGTC |
NGG |
2 |
0.4286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
131354036 |
+ |
Srgap2 |
NNNGGGGTGTAATACCTGTC |
NGG |
2 |
0.2143 |
Tier II |
| 2 |
NC_000069.6 |
3 |
57960299 |
+ |
Gm32753 |
NNNGGCCAGTAAAACCTGTC |
NGG |
2 |
0.476 |
Tier III |
| 3 |
NC_000069.6 |
3 |
57960299 |
+ |
Gm40055 |
NNNGGCCAGTAAAACCTGTC |
NGG |
2 |
0.476 |
Tier III |
| 4 |
NC_000067.6 |
1 |
131354036 |
+ |
Gm51644 |
NNNGGGGTGTAATACCTGTC |
NGG |
2 |
0.2143 |
Tier III |
| 5 |
NC_000068.7 |
2 |
57128985 |
+ |
BB557941 |
NNNGGCGTGTAATACCTGTC |
NAG |
2 |
0.1111 |
Tier III |
Other clones with same target sequence:
(none)
