GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001148588

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: GGACAAACTACATTCATGGG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: COL4A3BP (10087)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 77848

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000005.10	5	75506099	-	CERT1	NNNCAAACTACATTCATGGG	NGG	0	1.0	Tier I
2	NC_000019.10	19	45419161	-	ERCC1	NNNCAGACTACATCCATGGG	NGG	2	0.2041	Tier I
3	NC_000009.12	9	105466451	+	FSD1L	NNNAAAACAACATTCATGGG	NGG	2	0.782	Tier II
4	NC_000002.12	2	27784788	-	RBKS	NNNCAGACAACATTCATGGG	NGG	2	0.6633	Tier II
5	NC_000010.11	10	35306552	-	CCNY	NNNCAAACTTCATTCATGTG	NGG	2	0.5882	Tier II
6	NC_000005.10	5	158980690	-	EBF1	NNNCAAACTACATTCAAAGG	NGG	2	0.3692	Tier II
7	NC_000018.10	18	21060559	+	ROCK1	NNNCAAACTAAATTCATGGC	NGG	2	0.3214	Tier II
8	NC_000002.12	2	211875760	+	ERBB4	NNNCAAACTCCATTCATGGA	NGG	2	0.3125	Tier II
9	NC_000009.12	9	32427630	-	ACO1	NNNCAAACTCCATTCATGGT	NGG	2	0.2333	Tier II
10	NC_000020.11	20	25010583	-	ACSS1	NNNTAAACTAGATTCATGGG	NGG	2	0.2	Tier II
11	NC_000003.12	3	62149743	+	PTPRG	NNNCACACTACATTCATGGC	NGG	2	0.1948	Tier II
12	NC_000002.12	2	32089439	-	SPAST	NNNCAAACTACTTTCATGGG	NAG	2	0.0864	Tier II
13	NC_000008.11	8	56013619	+	LYN	NNNCAAACTACATCCAGGGG	NGG	2	0.0336	Tier II
14	NC_000005.10	5	180257948	+	MAPK9	NNNCAAACTACATGCATGGG	NGA	2	0.0	Tier II
15	NC_000006.12	6	72299103	-	RIMS1	NNNCAAACTACATTCATGGG	NTC	2	0.0	Tier II
16	NC_000003.12	3	53284340	+	DCP1A	NNNCAAACTACATTCTTTGG	NGG	2	0.0	Tier II
17	NC_000015.10	15	87613247	+	LOC102724465	NNNCAAGCCACATTCATGGG	NGG	2	0.6516	Tier III
18	NC_000010.11	10	35306552	-	LOC107984220	NNNCAAACTTCATTCATGTG	NGG	2	0.5882	Tier III
19	NC_000007.14	7	12015778	-	LOC124901589	NNNCAAACTACATTCATGAT	NGG	2	0.5	Tier III
20	NC_000004.12	4	117827804	-	LOC102723914	NNNCAAGCTACATTCATGGT	NGG	2	0.4941	Tier III
21	NC_000006.12	6	140620513	+	LOC105378026	NNNCATACTGCATTCATGGG	NGG	2	0.3968	Tier III
22	NC_000006.12	6	140620513	+	LOC105378027	NNNCATACTGCATTCATGGG	NGG	2	0.3968	Tier III
23	NC_000005.10	5	180257948	+	LOC100419721	NNNCAAACTACATGCATGGG	NGA	2	0.0	Tier III
24	NC_000009.12	9	80423917	-	LOC105376103	NNNCAAACTACATTCCTGGG	NGT	2	0.0	Tier III
25	NC_000003.12	3	53284340	+	LOC107986087	NNNCAAACTACATTCTTTGG	NGG	2	0.0	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000079.6	13	96549215	+	Cert1	NNNCAAACTATATTCATGGA	NGG	2	0.2885	Tier I
2	NC_000085.6	19	59903982	+	Rab11fip2	NNNAATACTACATTCATGGG	NGG	2	0.6015	Tier II
3	NC_000071.6	5	110697863	+	Ep400	NNNCCAACTACATTCATGGA	NGG	2	0.4688	Tier II
4	NC_000072.6	6	128562264	+	A2ml1	NNNGAAACTACATTCATGGA	NGG	2	0.4688	Tier II
5	NC_000073.6	7	19203278	+	Eml2	NNNGAAACTAAATTCATGGG	NGG	2	0.375	Tier II
6	NC_000069.6	3	108955543	-	Henmt1	NNNCAAACACCATTCATGGG	NGG	2	0.3095	Tier II
7	NC_000078.6	12	52294848	+	Nubpl	NNNCAAACTACATTCATGGT	NAG	2	0.1815	Tier II
8	NC_000072.6	6	32170796	+	Plxna4	NNNCAAACTACATTTATTGG	NGG	2	0.0444	Tier II
9	NC_000068.7	2	119542776	+	Exd1	NNNCAAACTACATTCAGGGG	NAG	2	0.0305	Tier II
10	NC_000068.7	2	155161031	-	Itch	NNNCAAAATACATTCATGGG	NTG	2	0.0253	Tier II
11	NC_000076.6	10	86422061	+	Syn3	NNNCAAACTACATTGATGGG	NTG	2	0.0019	Tier II
12	NC_000076.6	10	23250206	-	Eya4	NNNAAAACTACATTCCTGGG	NGG	2	0.0	Tier II
13	NC_000067.6	1	22390611	+	Rims1	NNNCAAACTACATTCATGGG	NTC	2	0.0	Tier II
14	NC_000071.6	5	26951804	-	Dpp6	NNNCAAACTACATTCCTGGG	NGG	1	0.0	Tier II
15	NC_000069.6	3	95885792	+	BC028528	NNNCAAACTACATTCTTGGG	NGC	2	0.0	Tier II
16	NC_000085.6	19	29637839	+	Ermp1	NNNCATACTACATGCATGGG	NGG	2	0.0	Tier II
17	NC_000069.6	3	17767824	-	Gm30340	NNNCAATCCACATTCATGGG	NGG	2	0.4038	Tier III
18	NC_000075.6	9	30986933	-	Gm29724	NNNCAAACTACACTCATGGG	NAG	2	0.2047	Tier III
19	NC_000069.6	3	95885792	+	C920021L13Rik	NNNCAAACTACATTCTTGGG	NGC	2	0.0	Tier III

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Other clones with same target sequence:

(none)