Construct: sgRNA BRDN0001148589
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTATTTGCATTGCAGCTGT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- DGUOK (1716)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000002.12 | 2 | 73938911 | + | DGUOK | NNNATTTGCATTGCAGCTGT | NGG | 0 | 1.0 | Tier I |
2 | NC_000018.10 | 18 | 33746456 | - | ASXL3 | NNNATCTGCATTGCTGCTGT | NGG | 2 | 0.1818 | Tier I |
3 | NC_000004.12 | 4 | 190185157 | - | DUX4 | NNNATTTGCTTTGCAACTGT | NGG | 2 | 0.8824 | Tier II |
4 | NC_000001.11 | 1 | 14021837 | - | KAZN | NNNATTTGTAATGCAGCTGT | NGG | 2 | 0.6563 | Tier II |
5 | NC_000001.11 | 1 | 147566251 | + | BCL9 | NNNATATGCATTGTAGCTGT | NGG | 2 | 0.6356 | Tier II |
6 | NC_000005.10 | 5 | 91093336 | - | ADGRV1 | NNNATTTGCTTTGCAGCAGT | NGG | 2 | 0.5882 | Tier II |
7 | NC_000012.12 | 12 | 2673144 | - | CACNA1C | NNNATTTGCTTTGCAGCAGT | NGG | 2 | 0.5882 | Tier II |
8 | NC_000005.10 | 5 | 156710030 | + | SGCD | NNNTTTAGCATTGCAGCTGT | NGG | 2 | 0.5568 | Tier II |
9 | NC_000005.10 | 5 | 159921515 | + | ADRA1B | NNNATGTGCATTGCAGCAGT | NGG | 2 | 0.381 | Tier II |
10 | NC_000001.11 | 1 | 8342561 | + | SLC45A1 | NNNATCTGCAGTGCAGCTGT | NGG | 2 | 0.3636 | Tier II |
11 | NC_000009.12 | 9 | 34093254 | + | DCAF12 | NNNATATGCAGTGCAGCTGT | NGG | 2 | 0.3467 | Tier II |
12 | NC_000003.12 | 3 | 2420711 | + | CNTN4 | NNNAAGTGCATTGCAGCTGT | NGG | 2 | 0.2857 | Tier II |
13 | NC_000008.11 | 8 | 64634990 | + | CYP7B1 | NNNAGTTGCAGTGCAGCTGT | NGG | 2 | 0.256 | Tier II |
14 | NC_000008.11 | 8 | 22931975 | + | PEBP4 | NNNCTTCGCATTGCAGCTGT | NGG | 2 | 0.2426 | Tier II |
15 | NC_000001.11 | 1 | 226267657 | - | LIN9 | NNNATTTTCATTGCAGCTGT | NAG | 2 | 0.162 | Tier II |
16 | NC_000006.12 | 6 | 136658876 | - | MAP3K5 | NNNATTTCCATTGCAGCTGT | NAG | 2 | 0.1595 | Tier II |
17 | NC_000007.14 | 7 | 103647643 | - | RELN | NNNATTTGCATTGAAGCTGT | NGA | 2 | 0.0243 | Tier II |
18 | NC_000011.10 | 11 | 34057056 | + | CAPRIN1 | NNNATTTTCATTGCAGCTGT | NGT | 2 | 0.0101 | Tier II |
19 | NC_000014.9 | 14 | 37279353 | + | MIPOL1 | NNNATTTGCATTAGAGCTGT | NGG | 2 | 0.0 | Tier II |
20 | NC_000010.11 | 10 | 126552453 | - | C10orf90 | NNNATTTGCATTGCAGCTGT | NTT | 2 | 0.0 | Tier II |
21 | NC_000002.12 | 2 | 113622855 | + | RPL23AP7 | NNNATTTGCTTTGCAACTGT | NGG | 2 | 0.8824 | Tier III |
22 | NC_000008.11 | 8 | 65007 | + | LOC101927506 | NNNATTTGCTTTGCAACTGT | NGG | 2 | 0.8824 | Tier III |
23 | NC_000016.10 | 16 | 90221555 | - | LINC02193 | NNNATTTGCTTTGCAACTGT | NGG | 2 | 0.8824 | Tier III |
24 | NC_000019.10 | 19 | 58588373 | - | LOC107987272 | NNNATTTGCTTTGCAACTGT | NGG | 2 | 0.8824 | Tier III |
25 | NC_000022.11 | 22 | 50788057 | - | RPL23AP82 | NNNATTTGCTTTGCAACTGT | NGG | 2 | 0.8824 | Tier III |
26 | NC_000015.10 | 15 | 32180312 | - | LOC102724078 | NNNATCTGAATTGCAGCTGT | NGG | 2 | 0.7792 | Tier III |
27 | NC_000005.10 | 5 | 156710030 | + | LOC105377673 | NNNTTTAGCATTGCAGCTGT | NGG | 2 | 0.5568 | Tier III |
28 | NC_000021.9 | 21 | 9111422 | - | TEKT4P2 | NNNATTTTCACTGCAGCTGT | NGG | 2 | 0.4167 | Tier III |
29 | NC_000003.12 | 3 | 13694768 | + | LINC00620 | NNNATTTGCACTGCAGCTGA | NGG | 2 | 0.375 | Tier III |
30 | NC_000003.12 | 3 | 150130424 | - | LOC105374313 | NNNATTTGGATTGCAGTTGT | NGG | 2 | 0.2889 | Tier III |
31 | NC_000003.12 | 3 | 106457166 | - | LOC101929485 | NNNATTTCCATTGCAGCTGT | NAG | 2 | 0.1595 | Tier III |
32 | NC_000013.11 | 13 | 112368870 | - | LOC105370373 | NNNATTTGCATTGTAGCTGT | NTG | 2 | 0.0286 | Tier III |
33 | NC_000023.11 | X | 39327314 | - | LINC01281 | NNNATTTGGATTGCAGCTGT | NGC | 2 | 0.0138 | Tier III |
34 | NC_000008.11 | 8 | 123753932 | - | LOC105375739 | NNNATTTGCATTGAATCTGT | NGG | 2 | 0.0 | Tier III |
35 | NC_000002.12 | 2 | 184336637 | - | LOC102724340 | NNNATTTGCATTGCACTTGT | NGG | 2 | 0.0 | Tier III |
36 | NC_000002.12 | 2 | 184336637 | - | LOC105373776 | NNNATTTGCATTGCACTTGT | NGG | 2 | 0.0 | Tier III |
37 | NC_000001.11 | 1 | 157311256 | - | LOC101928202 | NNNATTTGCATTGGAGATGT | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000072.6 | 6 | 83496798 | - | Dguok | NNNATTTGCATTGCAGCTGT | NGG | 0 | 1.0 | Tier I |
2 | NC_000071.6 | 5 | 88745058 | + | Mob1b | NNNATTTGCTTTGCAGCTGT | NGA | 2 | 0.0613 | Tier I |
3 | NC_000069.6 | 3 | 8818496 | - | Mrps28 | NNNATTTGCATTGTGGCTGT | NGG | 2 | 0.4767 | Tier II |
4 | NC_000068.7 | 2 | 168192172 | - | Adnp | NNNATTTTCATTGCAGCTAT | NGG | 2 | 0.4464 | Tier II |
5 | NC_000082.6 | 16 | 24397040 | + | Lpp | NNNATTTGTCTTGCAGCTGT | NGG | 2 | 0.2917 | Tier II |
6 | NC_000067.6 | 1 | 161782870 | - | Fasl | NNNATTTCCATTGCAGCTCT | NGG | 2 | 0.2759 | Tier II |
7 | NC_000072.6 | 6 | 110678450 | + | Grm7 | NNNATGTGCATTGCAGTTGT | NGG | 2 | 0.2667 | Tier II |
8 | NC_000083.6 | 17 | 24959146 | - | Jpt2 | NNNACTTGCATTGCTGCTGT | NGG | 2 | 0.2 | Tier II |
9 | NC_000067.6 | 1 | 46342881 | + | Dnah7b | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
10 | NC_000069.6 | 3 | 64205362 | + | Vmn2r1 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
11 | NC_000069.6 | 3 | 86841632 | + | Dclk2 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
12 | NC_000069.6 | 3 | 154176077 | - | Slc44a5 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
13 | NC_000070.6 | 4 | 42226737 | + | Ccl21b | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
14 | NC_000070.6 | 4 | 42231864 | + | Ccl21b | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
15 | NC_000072.6 | 6 | 47294415 | + | Cntnap2 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
16 | NC_000072.6 | 6 | 137544529 | - | Eps8 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
17 | NC_000075.6 | 9 | 44772218 | + | Ift46 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
18 | NC_000081.6 | 15 | 4560945 | - | Plcxd3 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
19 | NC_000083.6 | 17 | 6576086 | + | Gm29721 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
20 | NC_000085.6 | 19 | 22722763 | + | Trpm3 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
21 | NC_000085.6 | 19 | 22728110 | + | Trpm3 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier II |
22 | NC_000070.6 | 4 | 150284511 | + | Rere | NNNATTTGCATTCCAGGTGT | NGG | 2 | 0.0248 | Tier II |
23 | NC_000086.7 | X | 41420111 | + | Gria3 | NNNATTTGCATTCCAGGTGT | NGG | 2 | 0.0248 | Tier II |
24 | NC_000074.6 | 8 | 94854381 | + | Coq9 | NNNTTTTGCATTGCAGCTGT | NTG | 2 | 0.0248 | Tier II |
25 | NC_000082.6 | 16 | 74591790 | - | Robo2 | NNNATTTGCATTCCAGCTGT | NGC | 2 | 0.0094 | Tier II |
26 | NC_000078.6 | 12 | 57317290 | + | Mipol1 | NNNATTTGCATTAGAGCTGT | NGG | 2 | 0.0 | Tier II |
27 | NC_000068.7 | 2 | 75306219 | + | 2600014E21Rik | NNNATTTTCAATGCAGCTGT | NGG | 2 | 0.4688 | Tier III |
28 | NC_000068.7 | 2 | 75306219 | + | Gm39857 | NNNATTTTCAATGCAGCTGT | NGG | 2 | 0.4688 | Tier III |
29 | NC_000068.7 | 2 | 168192172 | - | Gm20716 | NNNATTTTCATTGCAGCTAT | NGG | 2 | 0.4464 | Tier III |
30 | NC_000079.6 | 13 | 80984671 | - | 9330111N05Rik | NNNTTTTGCATTGCTGCTGT | NGG | 2 | 0.1273 | Tier III |
31 | NC_000069.6 | 3 | 64205362 | + | Gm18294 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier III |
32 | NC_000073.6 | 7 | 71986792 | - | 1700011C11Rik | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier III |
33 | NC_000081.6 | 15 | 25010536 | + | Gm2824 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier III |
34 | NC_000082.6 | 16 | 91196661 | + | Gm41492 | NNNATTTGCATTGCAGCTTC | NGG | 2 | 0.0606 | Tier III |
35 | NC_000080.6 | 14 | 86304439 | + | Gm32860 | NNNATTTGCATTCCAGCTGT | NGA | 2 | 0.0292 | Tier III |
36 | NC_000071.6 | 5 | 45099745 | - | D5Ertd615e | NNNATTTGCACTGCAGCTGT | NGC | 2 | 0.0148 | Tier III |