Construct: sgRNA BRDN0001148589

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTATTTGCATTGCAGCTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGUOK (1716)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76604

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 73938911 + DGUOK NNNATTTGCATTGCAGCTGT NGG 0 1.0 Tier I
2 NC_000018.10 18 33746456 - ASXL3 NNNATCTGCATTGCTGCTGT NGG 2 0.1818 Tier I
3 NC_000004.12 4 190185157 - DUX4 NNNATTTGCTTTGCAACTGT NGG 2 0.8824 Tier II
4 NC_000001.11 1 14021837 - KAZN NNNATTTGTAATGCAGCTGT NGG 2 0.6563 Tier II
5 NC_000001.11 1 147566251 + BCL9 NNNATATGCATTGTAGCTGT NGG 2 0.6356 Tier II
6 NC_000005.10 5 91093336 - ADGRV1 NNNATTTGCTTTGCAGCAGT NGG 2 0.5882 Tier II
7 NC_000012.12 12 2673144 - CACNA1C NNNATTTGCTTTGCAGCAGT NGG 2 0.5882 Tier II
8 NC_000005.10 5 156710030 + SGCD NNNTTTAGCATTGCAGCTGT NGG 2 0.5568 Tier II
9 NC_000005.10 5 159921515 + ADRA1B NNNATGTGCATTGCAGCAGT NGG 2 0.381 Tier II
10 NC_000001.11 1 8342561 + SLC45A1 NNNATCTGCAGTGCAGCTGT NGG 2 0.3636 Tier II
11 NC_000009.12 9 34093254 + DCAF12 NNNATATGCAGTGCAGCTGT NGG 2 0.3467 Tier II
12 NC_000003.12 3 2420711 + CNTN4 NNNAAGTGCATTGCAGCTGT NGG 2 0.2857 Tier II
13 NC_000008.11 8 64634990 + CYP7B1 NNNAGTTGCAGTGCAGCTGT NGG 2 0.256 Tier II
14 NC_000008.11 8 22931975 + PEBP4 NNNCTTCGCATTGCAGCTGT NGG 2 0.2426 Tier II
15 NC_000001.11 1 226267657 - LIN9 NNNATTTTCATTGCAGCTGT NAG 2 0.162 Tier II
16 NC_000006.12 6 136658876 - MAP3K5 NNNATTTCCATTGCAGCTGT NAG 2 0.1595 Tier II
17 NC_000007.14 7 103647643 - RELN NNNATTTGCATTGAAGCTGT NGA 2 0.0243 Tier II
18 NC_000011.10 11 34057056 + CAPRIN1 NNNATTTTCATTGCAGCTGT NGT 2 0.0101 Tier II
19 NC_000014.9 14 37279353 + MIPOL1 NNNATTTGCATTAGAGCTGT NGG 2 0.0 Tier II
20 NC_000010.11 10 126552453 - C10orf90 NNNATTTGCATTGCAGCTGT NTT 2 0.0 Tier II
21 NC_000002.12 2 113622855 + RPL23AP7 NNNATTTGCTTTGCAACTGT NGG 2 0.8824 Tier III
22 NC_000008.11 8 65007 + LOC101927506 NNNATTTGCTTTGCAACTGT NGG 2 0.8824 Tier III
23 NC_000016.10 16 90221555 - LINC02193 NNNATTTGCTTTGCAACTGT NGG 2 0.8824 Tier III
24 NC_000019.10 19 58588373 - LOC107987272 NNNATTTGCTTTGCAACTGT NGG 2 0.8824 Tier III
25 NC_000022.11 22 50788057 - RPL23AP82 NNNATTTGCTTTGCAACTGT NGG 2 0.8824 Tier III
26 NC_000015.10 15 32180312 - LOC102724078 NNNATCTGAATTGCAGCTGT NGG 2 0.7792 Tier III
27 NC_000005.10 5 156710030 + LOC105377673 NNNTTTAGCATTGCAGCTGT NGG 2 0.5568 Tier III
28 NC_000021.9 21 9111422 - TEKT4P2 NNNATTTTCACTGCAGCTGT NGG 2 0.4167 Tier III
29 NC_000003.12 3 13694768 + LINC00620 NNNATTTGCACTGCAGCTGA NGG 2 0.375 Tier III
30 NC_000003.12 3 150130424 - LOC105374313 NNNATTTGGATTGCAGTTGT NGG 2 0.2889 Tier III
31 NC_000003.12 3 106457166 - LOC101929485 NNNATTTCCATTGCAGCTGT NAG 2 0.1595 Tier III
32 NC_000013.11 13 112368870 - LOC105370373 NNNATTTGCATTGTAGCTGT NTG 2 0.0286 Tier III
33 NC_000023.11 X 39327314 - LINC01281 NNNATTTGGATTGCAGCTGT NGC 2 0.0138 Tier III
34 NC_000008.11 8 123753932 - LOC105375739 NNNATTTGCATTGAATCTGT NGG 2 0.0 Tier III
35 NC_000002.12 2 184336637 - LOC102724340 NNNATTTGCATTGCACTTGT NGG 2 0.0 Tier III
36 NC_000002.12 2 184336637 - LOC105373776 NNNATTTGCATTGCACTTGT NGG 2 0.0 Tier III
37 NC_000001.11 1 157311256 - LOC101928202 NNNATTTGCATTGGAGATGT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 83496798 - Dguok NNNATTTGCATTGCAGCTGT NGG 0 1.0 Tier I
2 NC_000071.6 5 88745058 + Mob1b NNNATTTGCTTTGCAGCTGT NGA 2 0.0613 Tier I
3 NC_000069.6 3 8818496 - Mrps28 NNNATTTGCATTGTGGCTGT NGG 2 0.4767 Tier II
4 NC_000068.7 2 168192172 - Adnp NNNATTTTCATTGCAGCTAT NGG 2 0.4464 Tier II
5 NC_000082.6 16 24397040 + Lpp NNNATTTGTCTTGCAGCTGT NGG 2 0.2917 Tier II
6 NC_000067.6 1 161782870 - Fasl NNNATTTCCATTGCAGCTCT NGG 2 0.2759 Tier II
7 NC_000072.6 6 110678450 + Grm7 NNNATGTGCATTGCAGTTGT NGG 2 0.2667 Tier II
8 NC_000083.6 17 24959146 - Jpt2 NNNACTTGCATTGCTGCTGT NGG 2 0.2 Tier II
9 NC_000067.6 1 46342881 + Dnah7b NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
10 NC_000069.6 3 64205362 + Vmn2r1 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
11 NC_000069.6 3 86841632 + Dclk2 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
12 NC_000069.6 3 154176077 - Slc44a5 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
13 NC_000070.6 4 42226737 + Ccl21b NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
14 NC_000070.6 4 42231864 + Ccl21b NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
15 NC_000072.6 6 47294415 + Cntnap2 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
16 NC_000072.6 6 137544529 - Eps8 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
17 NC_000075.6 9 44772218 + Ift46 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
18 NC_000081.6 15 4560945 - Plcxd3 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
19 NC_000083.6 17 6576086 + Gm29721 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
20 NC_000085.6 19 22722763 + Trpm3 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
21 NC_000085.6 19 22728110 + Trpm3 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier II
22 NC_000070.6 4 150284511 + Rere NNNATTTGCATTCCAGGTGT NGG 2 0.0248 Tier II
23 NC_000086.7 X 41420111 + Gria3 NNNATTTGCATTCCAGGTGT NGG 2 0.0248 Tier II
24 NC_000074.6 8 94854381 + Coq9 NNNTTTTGCATTGCAGCTGT NTG 2 0.0248 Tier II
25 NC_000082.6 16 74591790 - Robo2 NNNATTTGCATTCCAGCTGT NGC 2 0.0094 Tier II
26 NC_000078.6 12 57317290 + Mipol1 NNNATTTGCATTAGAGCTGT NGG 2 0.0 Tier II
27 NC_000068.7 2 75306219 + 2600014E21Rik NNNATTTTCAATGCAGCTGT NGG 2 0.4688 Tier III
28 NC_000068.7 2 75306219 + Gm39857 NNNATTTTCAATGCAGCTGT NGG 2 0.4688 Tier III
29 NC_000068.7 2 168192172 - Gm20716 NNNATTTTCATTGCAGCTAT NGG 2 0.4464 Tier III
30 NC_000079.6 13 80984671 - 9330111N05Rik NNNTTTTGCATTGCTGCTGT NGG 2 0.1273 Tier III
31 NC_000069.6 3 64205362 + Gm18294 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier III
32 NC_000073.6 7 71986792 - 1700011C11Rik NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier III
33 NC_000081.6 15 25010536 + Gm2824 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier III
34 NC_000082.6 16 91196661 + Gm41492 NNNATTTGCATTGCAGCTTC NGG 2 0.0606 Tier III
35 NC_000080.6 14 86304439 + Gm32860 NNNATTTGCATTCCAGCTGT NGA 2 0.0292 Tier III
36 NC_000071.6 5 45099745 - D5Ertd615e NNNATTTGCACTGCAGCTGT NGC 2 0.0148 Tier III
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Other clones with same target sequence:

(none)