Construct: sgRNA BRDN0001148592
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGGAGCAGTGGTTTAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TSSK1B (83942)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76668
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000005.10 |
5 |
113434641 |
+ |
TSSK1B |
NNNGGAGCAGTGGTTTAACA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000005.10 |
5 |
113434641 |
+ |
MCC |
NNNGGAGCAGTGGTTTAACA |
NGG |
0 |
1.0 |
Tier II |
3 |
NC_000007.14 |
7 |
24864344 |
- |
OSBPL3 |
NNNCGAGCTGTGGTTTAACA |
NGG |
2 |
0.3176 |
Tier II |
4 |
NC_000006.12 |
6 |
109430143 |
+ |
PPIL6 |
NNNTGAGCAGTGGTTCAACA |
NGG |
2 |
0.2424 |
Tier II |
5 |
NC_000007.14 |
7 |
100376161 |
- |
PILRA |
NNNGGTGCAGTGGTTTACCA |
NGG |
2 |
0.1361 |
Tier II |
6 |
NC_000003.12 |
3 |
17160874 |
+ |
TBC1D5 |
NNNGAAGCAGTGGTTTAAGA |
NGG |
2 |
0.1083 |
Tier II |
7 |
NC_000005.10 |
5 |
172902318 |
+ |
ERGIC1 |
NNNGGAGCAGTGGCTTAACC |
NGG |
2 |
0.0649 |
Tier II |
8 |
NC_000015.10 |
15 |
67413331 |
- |
IQCH |
NNNGCAGCAGTGGTTTAACA |
NGC |
2 |
0.0175 |
Tier II |
9 |
NC_000009.12 |
9 |
129845443 |
+ |
USP20 |
NNNGGAGCAGAGGTTTAACA |
NGC |
2 |
0.0167 |
Tier II |
10 |
NC_000023.11 |
X |
68256003 |
+ |
OPHN1 |
NNNGGAGCAGAGGTTTAACA |
NGC |
2 |
0.0167 |
Tier II |
11 |
NC_000021.9 |
21 |
29328116 |
+ |
BACH1 |
NNNTGAGCAGTGGTTTAACA |
NTG |
2 |
0.0142 |
Tier II |
12 |
NC_000005.10 |
5 |
133810537 |
+ |
FSTL4 |
NNNGGAGCAGGGGTTTAACA |
NGC |
2 |
0.0089 |
Tier II |
13 |
NC_000005.10 |
5 |
113434641 |
+ |
LOC107986366 |
NNNGGAGCAGTGGTTTAACA |
NGG |
0 |
1.0 |
Tier III |
14 |
NC_000021.9 |
21 |
23363895 |
- |
D21S2088E |
NNNGGAGTAGTGGATTAACA |
NGG |
2 |
0.5417 |
Tier III |
15 |
NC_000002.12 |
2 |
211057099 |
- |
LOC107985978 |
NNNGGAGTAGTGGTTTACCA |
NGG |
2 |
0.1667 |
Tier III |
16 |
NC_000015.10 |
15 |
67413331 |
- |
IQCH-AS1 |
NNNGCAGCAGTGGTTTAACA |
NGC |
2 |
0.0175 |
Tier III |
17 |
NC_000001.11 |
1 |
192756365 |
+ |
LOC105371665 |
NNNGGAGCAGAGGTTTAACA |
NGC |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000069.6 |
3 |
103914861 |
- |
Rsbn1 |
NNNGGAGCAGAGGTTCAACA |
NGG |
2 |
0.5 |
Tier I |
2 |
NC_000082.6 |
16 |
5989863 |
- |
Rbfox1 |
NNNAGAGCAGAGGTTTAACA |
NGG |
2 |
0.675 |
Tier II |
3 |
NC_000072.6 |
6 |
137623428 |
- |
Eps8 |
NNNGGAGCAATGGATTAACA |
NGG |
2 |
0.5778 |
Tier II |
4 |
NC_000083.6 |
17 |
13728586 |
+ |
Tcte2 |
NNNCGAGCTGTGGTTTAACA |
NGG |
2 |
0.3176 |
Tier II |
5 |
NC_000080.6 |
14 |
17680273 |
+ |
Thrb |
NNNAGAGCAGTGTTTTAACA |
NGG |
2 |
0.27 |
Tier II |
6 |
NC_000083.6 |
17 |
67813761 |
+ |
Lama1 |
NNNGGATCAGTGGTTTAAAA |
NGG |
2 |
0.2449 |
Tier II |
7 |
NC_000078.6 |
12 |
72100562 |
+ |
Jkamp |
NNNGGAGCAGTGGTTTAGCT |
NGG |
2 |
0.24 |
Tier II |
8 |
NC_000073.6 |
7 |
16113292 |
- |
Napa |
NNNGGAGCAGTGCTTTAACA |
NAG |
2 |
0.1092 |
Tier II |
9 |
NC_000085.6 |
19 |
38540486 |
- |
Plce1 |
NNNGGAGCAGTGGTTTCTCA |
NGG |
2 |
0.0882 |
Tier II |
10 |
NC_000068.7 |
2 |
140716328 |
- |
Macrod2 |
NNNGGAGCAGTGGTTTGAGA |
NGG |
2 |
0.0221 |
Tier II |
11 |
NC_000086.7 |
X |
10589356 |
- |
Tspan7 |
NNNGGAGCAGTGCTGTAACA |
NGG |
2 |
0.0211 |
Tier II |
12 |
NC_000081.6 |
15 |
12229910 |
- |
Mtmr12 |
NNNGGAGCTGTGGTTTAACA |
NGC |
2 |
0.0133 |
Tier II |
13 |
NC_000082.6 |
16 |
91029563 |
+ |
Paxbp1 |
NNNGGAGCAGTTGTTTAACA |
NGC |
2 |
0.0085 |
Tier II |
14 |
NC_000082.6 |
16 |
5989863 |
- |
1700123O21Rik |
NNNAGAGCAGAGGTTTAACA |
NGG |
2 |
0.675 |
Tier III |
15 |
NC_000081.6 |
15 |
63471087 |
+ |
Gm20740 |
NNNGGATCAGTGGTTTAACT |
NGG |
2 |
0.3429 |
Tier III |
Other clones with same target sequence:
(none)