Construct: sgRNA BRDN0001148594

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGAGACATTGGGCTCCCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSK3A (2931)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76370

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 42234439 + GSK3A NNNAGACATTGGGCTCCCCT NGG 0 1.0 Tier I
2 NC_000002.12 2 227280961 - COL4A3 NNNAGTCCTTGGGCTCCCCT NGG 2 0.3061 Tier I
3 NC_000019.10 19 35559108 - ATP4A NNNAGACATTGGGCTCCACT NCG 2 0.0577 Tier I
4 NC_000021.9 21 46561015 - DIP2A NNNAGACACTGAGCTCCCCT NGG 2 0.8615 Tier II
5 NC_000001.11 1 160058560 - KCNJ10 NNNAAGCATTGGGCTCCCCT NGG 2 0.619 Tier II
6 NC_000019.10 19 1253077 + MIDN NNNAGACACTGGGCTCCCCA NGG 2 0.5192 Tier II
7 NC_000016.10 16 85285208 - GSE1 NNNTGACATTGGGTTCCCCT NGG 2 0.4667 Tier II
8 NC_000005.10 5 66681882 + MAST4 NNNGGGCATTGGGCTCCCCT NGG 2 0.4464 Tier II
9 NC_000001.11 1 3594108 - MEGF6 NNNGGACAGTGGGCTCCCCT NGG 2 0.3869 Tier II
10 NC_000016.10 16 78773436 + WWOX NNNAGACATTTGGCTCCCCT NGG 1 0.3846 Tier II
11 NC_000017.11 17 61055749 - BCAS3 NNNAGACATTAGGATCCCCT NGG 2 0.35 Tier II
12 NC_000009.12 9 34554547 + CNTFR NNNTGACATTGGCCTCCCCT NGG 2 0.2679 Tier II
13 NC_000002.12 2 218084045 + RUFY4 NNNAGACATTGGGCTCACCA NGG 2 0.2625 Tier II
14 NC_000020.11 20 61364968 - CDH4 NNNAGGCATTGGGATCCCCT NGG 2 0.25 Tier II
15 NC_000004.12 4 153715132 + RNF175 NNNAGACCTTGGGCTCCACT NGG 2 0.2308 Tier II
16 NC_000010.11 10 102629391 + SUFU NNNAGACATTTGGCTCTCCT NGG 2 0.1795 Tier II
17 NC_000002.12 2 168719185 + CERS6 NNNAGACATAGGGCTGCCCT NGG 2 0.1319 Tier II
18 NC_000014.9 14 90725622 - TTC7B NNNAGACCTTGGGCTCCCCT NAG 2 0.1111 Tier II
19 NC_000023.11 X 72601363 + PHKA1 NNNAGAGATTGGGCTCCCCT NTG 2 0.0183 Tier II
20 NC_000015.10 15 30151690 + DNM1P30 NNNAGGCACTGGGCTCCCCT NGG 2 0.6593 Tier III
21 NC_000016.10 16 85285208 - LINC00311 NNNTGACATTGGGTTCCCCT NGG 2 0.4667 Tier III
22 NC_000012.12 12 121107841 - LOC105370031 NNNAGGCAGTGGGCTCCCCT NGG 2 0.4422 Tier III
23 NC_000017.11 17 61055749 - BCAS3-AS1 NNNAGACATTAGGATCCCCT NGG 2 0.35 Tier III
24 NC_000002.12 2 227280961 - MFF-DT NNNAGTCCTTGGGCTCCCCT NGG 2 0.3061 Tier III
25 NC_000009.12 9 127939850 + LOC124902280 NNNAGACTTTGGGCTCACCT NGG 2 0.2 Tier III
26 NC_000012.12 12 30258396 - LINC02386 NNNAGACATTGGGCTCCCCT NTG 1 0.039 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 38191549 - Gsk3b NNNAAACATTGGGCTCTCCT NGG 2 0.4044 Tier I
2 NC_000085.6 19 47135530 + Calhm2 NNNAGACATAGGCCTCCCCT NGG 2 0.3609 Tier I
3 NC_000084.6 18 54981944 - Zfp608 NNNAAACATTGGGCTACCCT NGG 2 0.8667 Tier II
4 NC_000067.6 1 96861489 - Slco4c1 NNNAGACATTGGGTTACCCT NGG 2 0.7333 Tier II
5 NC_000081.6 15 77840006 - Myh9 NNNAGGCATTGGGCTACCCT NGG 2 0.7143 Tier II
6 NC_000079.6 13 96621820 + Cert1 NNNAGAAAATGGGCTCCCCT NGG 2 0.6964 Tier II
7 NC_000068.7 2 93220554 - Tspan18 NNNAGTCATTGGCCTCCCCT NGG 2 0.3008 Tier II
8 NC_000069.6 3 102778581 + Tshb NNNTGCCATTGGGCTCCCCT NGG 2 0.2893 Tier II
9 NC_000067.6 1 34047546 + Dst NNNGGACATTGGGCTCCCAT NGG 2 0.2679 Tier II
10 NC_000074.6 8 95059578 + Drc7 NNNAGACATTGTGCTCCCAT NGG 2 0.1648 Tier II
11 NC_000078.6 12 116491304 + Ptprn2 NNNAGACATTTGCCTCCCCT NGG 2 0.1619 Tier II
12 NC_000071.6 5 76660530 + Cracd NNNAGACACTGGGCTCCCCT NGA 2 0.0641 Tier II
13 NC_000078.6 12 84591058 - Vsx2 NNNAGACACTGGGGTCCCCT NGG 2 0.0 Tier II
14 NC_000078.6 12 79602000 + Rad51b NNNAGACATTGAGGTCCCCT NGG 2 0.0 Tier II
15 NC_000086.7 X 83530989 + Dmd NNNAGACTTTGGGGTCCCCT NGG 2 0.0 Tier II
16 NC_000068.7 2 93220554 - Gm32824 NNNAGTCATTGGCCTCCCCT NGG 2 0.3008 Tier III
17 NC_000083.6 17 16781660 + Gm5223 NNNAGACCTTTGGCTCCCCT NGG 2 0.1648 Tier III
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Other clones with same target sequence:

(none)