Construct: sgRNA BRDN0001148596

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTCACAAAGCTCCATCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK33 (65975)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76987

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 8457466 + STK33 NNNCACAAAGCTCCATCACA NGG 0 1.0 Tier I
2 NC_000002.12 2 15396444 - NBAS NNNCACAAAGCTTCATGACA NGG 2 0.0412 Tier I
3 NC_000001.11 1 166089639 - FAM78B NNNTACAAAACTCCATCACA NGG 2 0.7467 Tier II
4 NC_000001.11 1 13957100 - KAZN NNNCACAAGGCTCCAACACA NGG 2 0.6061 Tier II
5 NC_000009.12 9 130229977 + NCS1 NNNCATAATGCTCCATCACA NGG 2 0.5571 Tier II
6 NC_000015.10 15 51758456 + TMOD2 NNNCAGAAAGCTCCATCACA NGG 1 0.5 Tier II
7 NC_000023.11 X 104883769 + IL1RAPL2 NNNCCCAAAGCCCCATCACA NGG 2 0.4737 Tier II
8 NC_000013.11 13 112575080 + TUBGCP3 NNNCCCAAAACTCCATCACA NGG 2 0.4667 Tier II
9 NC_000005.10 5 111971819 + NREP NNNCAAAAAGCTCCTTCACA NGG 2 0.1857 Tier II
10 NC_000010.11 10 112522709 - VTI1A NNNCACACAGCTACATCACA NGG 2 0.1648 Tier II
11 NC_000002.12 2 166256748 + SCN9A NNNCACACAGTTCCATCACA NGG 2 0.1319 Tier II
12 NC_000019.10 19 36416191 + ZFP82 NNNCAAAAAGCTGCATCACA NGG 2 0.1266 Tier II
13 NC_000015.10 15 43800168 - SERINC4 NNNGACAAAGCTCCTTCACA NGG 2 0.1 Tier II
14 NC_000004.12 4 95187836 + UNC5C NNNCACAAAGCTGCAGCACA NGG 2 0.0472 Tier II
15 NC_000012.12 12 110639649 - TCTN1 NNNCACAAAACTCCATCACA NTG 2 0.0364 Tier II
16 NC_000015.10 15 92984944 + CHD2 NNNCACAAAGCTCCATCACA NAC 2 0.0 Tier II
17 NC_000016.10 16 80150843 - LOC105371357 NNNCAAAAAGCTCCAACACA NGG 2 0.8442 Tier III
18 NC_000001.11 1 166089639 - FAM78B-AS1 NNNTACAAAACTCCATCACA NGG 2 0.7467 Tier III
19 NC_000009.12 9 85493143 - STK33P1 NNNCACAGAGCCCCATCACA NGG 2 0.6947 Tier III
20 NC_000009.12 9 82345558 - LOC105376107 NNNCACAAATCTCTATCACA NGG 2 0.5958 Tier III
21 NC_000020.11 20 23812913 + LOC105372575 NNNCCCAAAGCCCCATCACA NGG 2 0.4737 Tier III
22 NC_000007.14 7 88337260 - LOC107986815 NNNCACAAATCTCCATCTCA NGG 2 0.4063 Tier III
23 NC_000003.12 3 55301365 + LOC124906243 NNNCACAAAGATCCAGCACA NGG 2 0.2596 Tier III
24 NC_000005.10 5 111971819 + NREP-AS1 NNNCAAAAAGCTCCTTCACA NGG 2 0.1857 Tier III
25 NC_000006.12 6 41107472 + ADCY10P1 NNNTACAAAGCTCCTTCACA NGG 2 0.16 Tier III
26 NC_000002.12 2 166256748 + SCN1A-AS1 NNNCACACAGTTCCATCACA NGG 2 0.1319 Tier III
27 NC_000015.10 15 43800168 - SERF2-C15ORF63 NNNGACAAAGCTCCTTCACA NGG 2 0.1 Tier III
28 NC_000006.12 6 127469782 - SOGA3-KIAA0408 NNNCACACAGCTCCATCACA NTG 2 0.0167 Tier III
29 NC_000002.12 2 114009832 - LINC01191 NNNCACAAAGCTGCATCACA NTG 2 0.0053 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 100154909 - Naip2 NNNCCCAAACCTCCATCACA NGG 2 0.2 Tier I
2 NC_000073.6 7 109331594 + Stk33 NNNCACAAAGCTCCATCACG NGA 2 0.0531 Tier I
3 NC_000072.6 6 52906551 + Jazf1 NNNCACAAAGCTCCATCACT NGG 1 0.6 Tier II
4 NC_000070.6 4 88351804 + Focad NNNCACAAATCTCCATCTCA NGG 2 0.4063 Tier II
5 NC_000073.6 7 49341071 - Nav2 NNNCACAAGGCTCCATCTCA NGG 2 0.3333 Tier II
6 NC_000075.6 9 42467108 + Tbcel NNNCACAAAGCTCAAACACA NGG 2 0.3182 Tier II
7 NC_000082.6 16 3940659 + Cluap1 NNNCACAAACCTCCACCACA NGG 2 0.2667 Tier II
8 NC_000068.7 2 152696642 + H13 NNNCCCAAAGCTCCATCTCA NGG 2 0.25 Tier II
9 NC_000077.6 11 104804389 - Gm11639 NNNCACAAAGCTCCAACACA NAG 2 0.2357 Tier II
10 NC_000076.6 10 111193290 - Osbpl8 NNNCACAAAGCCCCATCACC NGG 2 0.2153 Tier II
11 NC_000070.6 4 103924719 + Dab1 NNNCACAAATCTCCATCACA NAG 2 0.2106 Tier II
12 NC_000069.6 3 152208307 + Dnajb4 NNNCACAAAGCACCATCACC NGG 2 0.1818 Tier II
13 NC_000083.6 17 90721251 + Nrxn1 NNNGTCAAAGCTCCATCACA NGG 2 0.1818 Tier II
14 NC_000071.6 5 107800988 + Evi5 NNNCACTAAGCTACATCACA NGG 2 0.1683 Tier II
15 NC_000083.6 17 47325509 - Trerf1 NNNTACAAAGCTCCATCCCA NGG 2 0.1524 Tier II
16 NC_000070.6 4 118615518 - Cfap57 NNNCACAACGCTCCATCACA NAG 2 0.1481 Tier II
17 NC_000074.6 8 17014488 + Csmd1 NNNCTCAAAGCTCCATCGCA NGG 2 0.1455 Tier II
18 NC_000068.7 2 179625012 + Cdh4 NNNCACATAGCTCCATCACC NGG 2 0.0974 Tier II
19 NC_000070.6 4 46848208 + Gabbr2 NNNGACAAAGCTCCATCCCA NGG 2 0.0952 Tier II
20 NC_000068.7 2 130591507 + Ubox5 NNNCACAAAGCTCCATTAGA NGG 2 0.0583 Tier II
21 NC_000080.6 14 117551979 - Gpc6 NNNTACAAAGCTCCATCACA NGA 2 0.0556 Tier II
22 NC_000081.6 15 79300599 + Pla2g6 NNNGACAAAGCTCCATGACA NGG 2 0.0294 Tier II
23 NC_000069.6 3 27375350 + Ghsr NNNCACAAAGCTCCATCACA NGC 1 0.0222 Tier II
24 NC_000083.6 17 24901837 + Mapk8ip3 NNNCACAAAGCTCCATCTCA NTG 2 0.0195 Tier II
25 NC_000082.6 16 25967661 + P3h2 NNNCACAAAGCTCCTTCACA NGT 2 0.0032 Tier II
26 NC_000069.6 3 115641046 - Gm40124 NNNCACAAAACTCCATCTCA NGG 2 0.4667 Tier III
27 NC_000073.6 7 49341071 - Gm51448 NNNCACAAGGCTCCATCTCA NGG 2 0.3333 Tier III
28 NC_000075.6 9 42467108 + Gm16322 NNNCACAAAGCTCAAACACA NGG 2 0.3182 Tier III
29 NC_000072.6 6 30170956 - Mirc35hg NNNCACAAAGCTCCATTTCA NGG 2 0.2333 Tier III
30 NC_000076.6 10 73002584 - A330049N07Rik NNNCACAAAGCTCCAGCACT NGG 2 0.2077 Tier III
31 NC_000069.6 3 152208307 + Gm40180 NNNCACAAAGCACCATCACC NGG 2 0.1818 Tier III
32 NC_000082.6 16 15010477 + Gm46561 NNNCACAAAGCTCTATCACA NTG 2 0.0286 Tier III
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Other clones with same target sequence:

(none)