Construct: sgRNA BRDN0001148596
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTCACAAAGCTCCATCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK33 (65975)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76987
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000079.6 |
13 |
100154909 |
- |
Naip2 |
NNNCCCAAACCTCCATCACA |
NGG |
2 |
0.2 |
Tier I |
2 |
NC_000073.6 |
7 |
109331594 |
+ |
Stk33 |
NNNCACAAAGCTCCATCACG |
NGA |
2 |
0.0531 |
Tier I |
3 |
NC_000072.6 |
6 |
52906551 |
+ |
Jazf1 |
NNNCACAAAGCTCCATCACT |
NGG |
1 |
0.6 |
Tier II |
4 |
NC_000070.6 |
4 |
88351804 |
+ |
Focad |
NNNCACAAATCTCCATCTCA |
NGG |
2 |
0.4063 |
Tier II |
5 |
NC_000073.6 |
7 |
49341071 |
- |
Nav2 |
NNNCACAAGGCTCCATCTCA |
NGG |
2 |
0.3333 |
Tier II |
6 |
NC_000075.6 |
9 |
42467108 |
+ |
Tbcel |
NNNCACAAAGCTCAAACACA |
NGG |
2 |
0.3182 |
Tier II |
7 |
NC_000082.6 |
16 |
3940659 |
+ |
Cluap1 |
NNNCACAAACCTCCACCACA |
NGG |
2 |
0.2667 |
Tier II |
8 |
NC_000068.7 |
2 |
152696642 |
+ |
H13 |
NNNCCCAAAGCTCCATCTCA |
NGG |
2 |
0.25 |
Tier II |
9 |
NC_000077.6 |
11 |
104804389 |
- |
Gm11639 |
NNNCACAAAGCTCCAACACA |
NAG |
2 |
0.2357 |
Tier II |
10 |
NC_000076.6 |
10 |
111193290 |
- |
Osbpl8 |
NNNCACAAAGCCCCATCACC |
NGG |
2 |
0.2153 |
Tier II |
11 |
NC_000070.6 |
4 |
103924719 |
+ |
Dab1 |
NNNCACAAATCTCCATCACA |
NAG |
2 |
0.2106 |
Tier II |
12 |
NC_000069.6 |
3 |
152208307 |
+ |
Dnajb4 |
NNNCACAAAGCACCATCACC |
NGG |
2 |
0.1818 |
Tier II |
13 |
NC_000083.6 |
17 |
90721251 |
+ |
Nrxn1 |
NNNGTCAAAGCTCCATCACA |
NGG |
2 |
0.1818 |
Tier II |
14 |
NC_000071.6 |
5 |
107800988 |
+ |
Evi5 |
NNNCACTAAGCTACATCACA |
NGG |
2 |
0.1683 |
Tier II |
15 |
NC_000083.6 |
17 |
47325509 |
- |
Trerf1 |
NNNTACAAAGCTCCATCCCA |
NGG |
2 |
0.1524 |
Tier II |
16 |
NC_000070.6 |
4 |
118615518 |
- |
Cfap57 |
NNNCACAACGCTCCATCACA |
NAG |
2 |
0.1481 |
Tier II |
17 |
NC_000074.6 |
8 |
17014488 |
+ |
Csmd1 |
NNNCTCAAAGCTCCATCGCA |
NGG |
2 |
0.1455 |
Tier II |
18 |
NC_000068.7 |
2 |
179625012 |
+ |
Cdh4 |
NNNCACATAGCTCCATCACC |
NGG |
2 |
0.0974 |
Tier II |
19 |
NC_000070.6 |
4 |
46848208 |
+ |
Gabbr2 |
NNNGACAAAGCTCCATCCCA |
NGG |
2 |
0.0952 |
Tier II |
20 |
NC_000068.7 |
2 |
130591507 |
+ |
Ubox5 |
NNNCACAAAGCTCCATTAGA |
NGG |
2 |
0.0583 |
Tier II |
21 |
NC_000080.6 |
14 |
117551979 |
- |
Gpc6 |
NNNTACAAAGCTCCATCACA |
NGA |
2 |
0.0556 |
Tier II |
22 |
NC_000081.6 |
15 |
79300599 |
+ |
Pla2g6 |
NNNGACAAAGCTCCATGACA |
NGG |
2 |
0.0294 |
Tier II |
23 |
NC_000069.6 |
3 |
27375350 |
+ |
Ghsr |
NNNCACAAAGCTCCATCACA |
NGC |
1 |
0.0222 |
Tier II |
24 |
NC_000083.6 |
17 |
24901837 |
+ |
Mapk8ip3 |
NNNCACAAAGCTCCATCTCA |
NTG |
2 |
0.0195 |
Tier II |
25 |
NC_000082.6 |
16 |
25967661 |
+ |
P3h2 |
NNNCACAAAGCTCCTTCACA |
NGT |
2 |
0.0032 |
Tier II |
26 |
NC_000069.6 |
3 |
115641046 |
- |
Gm40124 |
NNNCACAAAACTCCATCTCA |
NGG |
2 |
0.4667 |
Tier III |
27 |
NC_000073.6 |
7 |
49341071 |
- |
Gm51448 |
NNNCACAAGGCTCCATCTCA |
NGG |
2 |
0.3333 |
Tier III |
28 |
NC_000075.6 |
9 |
42467108 |
+ |
Gm16322 |
NNNCACAAAGCTCAAACACA |
NGG |
2 |
0.3182 |
Tier III |
29 |
NC_000072.6 |
6 |
30170956 |
- |
Mirc35hg |
NNNCACAAAGCTCCATTTCA |
NGG |
2 |
0.2333 |
Tier III |
30 |
NC_000076.6 |
10 |
73002584 |
- |
A330049N07Rik |
NNNCACAAAGCTCCAGCACT |
NGG |
2 |
0.2077 |
Tier III |
31 |
NC_000069.6 |
3 |
152208307 |
+ |
Gm40180 |
NNNCACAAAGCACCATCACC |
NGG |
2 |
0.1818 |
Tier III |
32 |
NC_000082.6 |
16 |
15010477 |
+ |
Gm46561 |
NNNCACAAAGCTCTATCACA |
NTG |
2 |
0.0286 |
Tier III |
Other clones with same target sequence:
(none)