Construct: sgRNA BRDN0001148597
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGCGAGTGTAGGTACTGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKACG (5568)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77364
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
69013627 |
+ |
PRKACG |
NNNCGAGTGTAGGTACTGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
17159795 |
- |
TBC1D5 |
NNNCAAGTGTAGGTACTGCA |
NGG |
2 |
0.3885 |
Tier II |
| 3 |
NC_000006.12 |
6 |
31795547 |
+ |
VARS1 |
NNNCGCGTGTACGTACTGGA |
NGG |
2 |
0.2406 |
Tier II |
| 4 |
NC_000006.12 |
6 |
12011758 |
- |
HIVEP1 |
NNNCGGGTGTAGGGACTGGA |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000009.12 |
9 |
70232525 |
- |
SMC5-DT |
NNNAGGGTGTAGGTACTGGA |
NGG |
2 |
0.6015 |
Tier III |
| 6 |
NC_000004.12 |
4 |
37105701 |
- |
LOC101928721 |
NNNCGAGTGGAGTTACTGGA |
NGG |
2 |
0.15 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
131058042 |
+ |
Mapkapk2 |
NNNCGAGTGCAGGTACTGGA |
NGG |
1 |
0.5333 |
Tier I |
| 2 |
NC_000075.6 |
9 |
35511482 |
- |
Gm1113 |
NNNCGAGTGTCAGTACTGGA |
NGG |
2 |
0.3733 |
Tier I |
| 3 |
NC_000073.6 |
7 |
109715885 |
- |
BC051019 |
NNNCGAGTGGAGGTACTGGA |
NGA |
2 |
0.0347 |
Tier I |
| 4 |
NC_000077.6 |
11 |
49847839 |
- |
Mapk9 |
NNNCCAGAGTAGGTACTGGA |
NGG |
2 |
0.6286 |
Tier II |
| 5 |
NC_000076.6 |
10 |
77855763 |
+ |
Tspear |
NNNAGAGTGTGGGTACTGGA |
NGG |
2 |
0.5474 |
Tier II |
| 6 |
NC_000082.6 |
16 |
97829985 |
- |
Prdm15 |
NNNCAAGTGTAGGTCCTGGA |
NGG |
2 |
0.2364 |
Tier II |
| 7 |
NC_000067.6 |
1 |
93590354 |
- |
Farp2 |
NNNAGAGTGTAGGTCCTGGA |
NGG |
2 |
0.2297 |
Tier II |
| 8 |
NC_000077.6 |
11 |
82920190 |
- |
Unc45b |
NNNCGAGTGTAGGGACTGCA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000077.6 |
11 |
63100437 |
+ |
Gm36926 |
NNNTGAGTGTAGGTACTGAA |
NGG |
2 |
0.5714 |
Tier III |
| 10 |
NC_000083.6 |
17 |
25958339 |
+ |
D630044L22Rik |
NNNTGTGTGTAGGTACTGGA |
NGG |
2 |
0.5714 |
Tier III |
Other clones with same target sequence:
(none)
